Determination of Eight Sudan Dyes in Chili Powder by UPLC-MS/MS

Abstract

Sudan dyes are synthetic azo dyes which are widely used in industry. Although they are not allowed in foodstuffs, they have been found contaminating in different food products and their presence is regularly reported. It is assumed that these appearances are due to cross-contamination or adulteration. In this paper, we present a newly developed fast and sensitive method for the quantification of eight Sudan dyes, using liquid-liquid extraction and UPLC-MS/MS analysis. The calibration curves were linear over the range of 0.1 -25 mg/kg. Mean recovery for the eight Sudan dyes ranged from 80.7% to 104.4%, and with the inter-day and intra-day precisions ranged from 2.24% to 12.2%. The method was successfully applied in the determination of Sudan dyes in chili powder of 10 samples.

Fast, easy, cheap, robust and safe method of analysis of Sudan dyes in chilli pepper powder

“…(2017) Ultra-high-performance Supercritical fluid chromatography (UHPSFC)-PDA 0.10–0.30 <8.0 82.6–108.3 Yang et al. (2019) Centrifugation-UPLC-MS/MS 0.003–0.03 2.24–12.2 80.7–104.4 Wang et al. (2013) …”

“…Our profound gratitude goes to the University of Cape Coast, especially to the School of Physical Sciences, for making their laboratory and instruments available for the study. Centrifugation-UPLC-MS/MS 0.003-0.03 2.24-12.2 80.7-104.4 Wang et al (2013)…”

Analysis of 13 Banned Colorants in Cosmetics via Liquid Chromatographic and Mass Spectrometric Techniques

“…Compared to natural colorants, synthetic ones exhibit the advantages of long-lasting effect, lower price, and higher stability to light, oxygen, and pH, and are therefore typically the preferred choice [10][11][12][13][14][15][16]. However, most synthetic colorants contain azo functional groups or aromatic rings and can therefore cause allergies, asthma, DNA damage, and even cancer or mutagenesis [11,12,[14][15][16][17][18][19][20][21][22]. Therefore, the types and standards of colorants allowed in cosmetics are closely regulated.…”

“…Previous studies on pigment analysis employed high-performance liquid chromatography (HPLC), ultra-high-performance liquid chromatography (UHPLC), ultra-high-performance supercritical fluid chromatography, and liquid chromatography-electrospray ionization tandem mass spectrometry [12,13,15,[21][22][23][24][25][26][27][28][29][30], but have mostly focused on food or textiles and not cosmetics. Guerra et al used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to simultaneously analyze nine water-soluble pigments found in cosmetics/personal care products and detected seven of these targets at levels of 0.054-580 µg/g [24].…”

Development and validation of an LC‐MS/MS method for the determination of bullatine A in rat plasma: application to a pharmacokinetic study

“…Moreover, LC-MS/MS is considered a gold standard technique for drug analysis in biological samples. However, the reported method encounters several disadvantages, such as employing an external standard (no internal standard, IS, was used), time-consuming elution procedures, large sample volumes, inappropriate QC samples, incomplete validation procedures and inadequate LLOQs (Wang et al, 2013). In addition, this method could presumably be appropriate for therapeutic drug monitoring and does not provide any pharmacokinetic data.…”