Determination of Effects of Sample Processing on Hibiscus sabdariffa L. Using Tri-step Infrared Spectroscopy

Choong, Yew Keong; Yousof, Nor Syaidatul Akmal Mohd; Wasiman, Mohd Isa; Jamal, Jamia Azdina; Ismail, Zhari

doi:10.4172/2155-9872.1000335

Cited by 10 publications

(6 citation statements)

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“…24 In addition, the usage of TFA in the extraction can stabilise the extracted component. 25 Daily administration of ARTE at 300 mg/kg daily for 3 weeks showed a significant reduction in BMI similar to previous study. 18 Although Roselle is potential as an antiobesity, the exact mechanisms that cause reduction in BMI are not fully understood.…”

Section: Discussionsupporting

confidence: 89%

Therapeutic effects of anthocyanin-rich Hibiscus sabdariffa L. extract on body mass index, lipid profile and fatty liver in obese-hypercholesterolaemic rat model

Noordin¹,

Noor²,

Safuan³

et al. 2019

Int J Basic Clin Pharmacol

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Background: Hibiscus sabdariffa L. or Roselle, is one of the most common flower plants cultivated worldwide. Traditionally, it is claimed to reduce weight and cholesterol level. The aim of this study was to evaluate the effects of anthocyanin-rich Roselle aqueous extract on the physical, biochemical and histological changes in obese-hypercholesterolaemic rat model.Methods: Thirty Sprague-Dawley male rats were divided into five groups (n=6 per group). Group 1 is normal control (NC) rat that was fed with normal diet. The remaining rats (Group 2-5) were fed with commercial high-fat diet (HFD) for 6 weeks to induce obese-hypercholesterolaemic (OH) rat. After induction, the OH rats were divided into 4 groups: OH-Untreated group, OH-ARTE; OH treated with 300 mg/kg Roselle aqueous (anthocyanin-rich) and 1% trifluoroacetic acid extract (ARTE), OH-Orlistat; OH treated with 37 mg/kg orlistat and OH-Atorvastatin; OH treated with 10 mg/kg atorvastatin. After the 3 weeks intervention period, all rats were sacrificed. Body mass index, lipid profile and liver enzymes were evaluated. The liver section was subjected to hematoxylin and eosin staining for histopathological evaluation.Results: This study showed that 3 weeks administration of ARTE significantly reduced BMI, improved levels of lipid profile and liver enzymes, and histopathological changes of liver when compared to OH-untreated rats. The results were also comparable to the standard drugs.Conclusions: This study suggested that Roselle extract constitutes an effective and safe alternate treatment for obesity and hyperlipidaemia.

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Section: Discussionsupporting

confidence: 89%

Therapeutic effects of anthocyanin-rich Hibiscus sabdariffa L. extract on body mass index, lipid profile and fatty liver in obese-hypercholesterolaemic rat model

Noordin¹,

Noor²,

Safuan³

et al. 2019

Int J Basic Clin Pharmacol

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“…In the infrared fingerprint region, several absorption peaks features can be extracted such as the peak at 1607 cm −1 was assigned to the C=C bonds stretching vibration in aromatic rings compounds, and the peaks at 1221 -1030 cm −1 were attributed to C-O stretching vibration, which displayed the fingerprint features of glycosides. In addition, the strong peak at 1800 and 1700 cm −1 in both fingerprint area are corresponded to the stretching vibrations of C=O group, normally refers to ester components or acid anhydride group [25]. These data indicate that the major content in UKMR-2 calyces have their own infrared characteristic peaks.…”

Section: Conventional Infrared (1d-ir) Spectrum Analysis Of Ukmr-2 Camentioning

confidence: 66%

“…The present study of 1D-IR spectrum UKMR-2 calyx spectrum was also compared with H. sabdariffa L. extract from Choong et al [25] and anthocyanin standard. Both roselle varieties have a similar pattern of peaks from the range of 1800 -1600 cm -1 .…”

Section: Conventional Infrared (1d-ir) Spectrum Analysis Of Ukmr-2 Camentioning

confidence: 99%

RAPID INVESTIGATION OF THE METABOLITE CONTENT IN HIBISCUS SABDARIFFA var. UKMR-2 CULTIVATED UNDER THE INFLUENCE OF ELEVATED CO2 USING TRI-STEP FT-IR SPECTROSCOPY

Ali

Latip

2020

Jurnal Teknologi

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Rapid methods based on untargeted analysis technique such as Fourier Transform Infrared (FT-IR) spectroscopy can provide much faster and easier solution for food authentication. However, studies on the metabolite content in UKMR-2 calyces using FT-IR spectroscopy has not been reported yet in any previous studies. Thus, the present study was performed to analyze the differences in metabolite content in UKMR-2 calyces under the influences of different [CO2] treatment by applying tri-step infrared based fingerprinting. The UKMR-2 plant cultivation was exposed to ambient [CO2] (400 µmol/mol) and elevated [CO2] (800 µmol/mol) treatment. The UKMR-2 calyx extracts were analysed by conventional infrared (1D-IR), second derivative infrared (SD-IR) and two-dimensional correlation infrared (2D-IR) spectroscopy. The 1D-IR spectrum results revealed a similar absorption spectrum in the range of 1900 - 650 cm-1, which suggest similar major metabolites content present in both extracts. For SD-IR spectrum, both treatments clearly showed have more peaks with different shape, position and intensity in the range of 1650 - 1450 cm-1 and 1200 - 950 cm-1, which is likely to have different flavonoid and carbohydrate content in UKMR-2 calyces. The 2D-IR synchronous correlation spectrum in the range of 1000 – 650 cm-1 clearly distinguished the metabolite content in the UKMR-2 calyx extract from different [CO2] treatment. Therefore, this tri-step infrared based fingerprinting has the potential as one of the effective methods to discriminate extract samples with similar infrared fingerprint features and indicate that the metabolite content in UKMR-2 calyces were influenced by different [CO2] treatments.

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“…The main peaks observed in the two samples were: ~ 1265 cm -1 , attributed to the stretching of O-C acid groups (Johson et al, 2017). Peaks between 1100 and 1071 cm -1 , indicate the presence of anthocyanins (cyanidin-3-O-sambubioside and delphinidin-3-O-sambubioside) showing the presence of these compounds in the samples (Choong et al, 2016). The region between 3100 and 3500 cm -1 is attributed to O-H stretch vibration (Lee et al, 2013).…”

Section: Extract Charaterization (Ftir-atr)mentioning

confidence: 98%

Hibiscus sabdariffa L. Extract: Characterization (FTIR-ATR), Storage Stability and Food Application

Paraíso¹,

Santos

Ogawa

et al. 2020

Emir J Food Agric

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The objective was to evaluate the extract stability of hibiscus in different conditions and the food application. Anthocyanins (TA), phenolic compounds (TP) and color, at: 4 and 25 °C, presence or absence of light, pH stability and FTIR were evaluated. Gelatin developed with hibiscus extract was submitted to sensory analysis. Light and temperature factors affected the stability of TP and TA throughout the storage. Higher stability was observed at 4 ºC and absence of light (in relation to TA and TP respectively, 3% and 26% losses were observed in this condition against 43% and 39% at 25 °C with light). Regarding pH, it was observed that pH change affected the absorbance and color in the hibiscus extract. Finally, the hibiscus extract, when added at the highest concentration into gelatin, was the most accepted by tastes, and scored 7 (like moderately) for color and global acceptance.

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Determination of Effects of Sample Processing on Hibiscus sabdariffa L. Using Tri-step Infrared Spectroscopy

Cited by 10 publications

References 0 publications

Therapeutic effects of anthocyanin-rich Hibiscus sabdariffa L. extract on body mass index, lipid profile and fatty liver in obese-hypercholesterolaemic rat model

Therapeutic effects of anthocyanin-rich Hibiscus sabdariffa L. extract on body mass index, lipid profile and fatty liver in obese-hypercholesterolaemic rat model

RAPID INVESTIGATION OF THE METABOLITE CONTENT IN HIBISCUS SABDARIFFA var. UKMR-2 CULTIVATED UNDER THE INFLUENCE OF ELEVATED CO2 USING TRI-STEP FT-IR SPECTROSCOPY

Hibiscus sabdariffa L. Extract: Characterization (FTIR-ATR), Storage Stability and Food Application

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