Determination of DNA damage after exposure to inhalation anesthetics in human peripheral lymphocytes and sperm cells in vitro by comet assay

Kaymak, Çetin; Kadıoğlu, Ela; Coşkun, Erdem; Başar, Hülya; Başar, M. Murad

doi:10.1177/0960327112446818

Cited by 24 publications

(24 citation statements)

References 35 publications

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“…Abnormalities and malfunctions associated with these hormones manifest themselves by impairments in sperm characteristics. Many factors, such as testicular torsion, infections, cryptorchidism, gonadal dysgenesis, varicocele, medication, and EMR can unfavorably affect spermatogenesis and lead to infertility (8). Chronic exposure to radiofrequency electromagnetic radiation of cell phone leads to defective testicular function that is associated with increased oxidative stress (9) and decreased gonadotropic hormones (FSH, LH, T) profi le (10).…”

Section: Introductionmentioning

confidence: 99%

Inhibition by Egb761 of the effect of cellphone radiation on the male reproductive system

Gevrek¹,

Aydın²,

Ozsoy³

et al. 2017

BLL

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OBJECTIVES: To determine the effects of Egb761 on testicular tissues and semen parameters in rats exposed to cellphone waves. BACKGROUND: EGb761 has antioxidant properties as a free-radical scavenger. Cellphone electromagnetic radiation (EMR) induces oxidative stress in cells. METHODS: Twenty-one Wistar albino male adult rats were divided into three groups (control, experimental, treatment), including seven rats in each. The experimental and treatment groups were exposed to cellphone EMR (0.96 W/kg) for six weeks (4 hrs/day). Egb761 (100 mg/kg/day) was also added to the treatment. Testes, epididymal semen and blood plasma were used for analysis. RESULTS: Exposure to cellular phone radiation resulted in a signifi cant impairment in testicular morphometry and histological structure, reduction of total and motile sperm numbers and plasma testosterone level. Egb761 administration improved testicular damage and led to a marked increase in plasma testosterone levels and total and motile sperm numbers. CONCLUSION: Male reproductive system is susceptible to cellphone radiation. Cellphone waves induce toxic effects in testicular tissues, impair spermatogenesis and cause an imbalance in testosterone hormone levels. Egb761 ameliorated these toxic effects by reversing testicular tissue damage, restoring normal spermatogenesis and hormone levels. This suggests that Egb761 is a potential therapeutic agent against EMR-induced male reproductive toxicity (Tab. 3, Fig. 6, Ref. 45). Text in PDF www.elis.sk.

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Section: Introductionmentioning

confidence: 99%

Inhibition by Egb761 of the effect of cellphone radiation on the male reproductive system

Gevrek¹,

Aydın²,

Ozsoy³

et al. 2017

BLL

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“…epithelial cells in the nose and bronchoalveolar cells [ 57 , 58 ] and systemically, e.g. peripheral blood lymphocytes (PBLs) [ 56 , 59–64 ] although those risks seem to be agent and dose/duration dependent [ 56 , 65 ]. GA waste gases are even seen to induce DNA damage in operating room personnel, with chronic exposure inducing cumulative genotoxic effects [ 66 , 67 ].…”

Section: Cellular Genetic and Epigenetic Toxicities Of Svagsmentioning

confidence: 99%

“…We could locate just a single study of DNA damage in germ cells caused by these chemicals, and it was performed on human sperm in vitro [ 65 ]. In this study, genotoxic activities of different concentrations of halothane, isoflurane, sevoflurane and desflurane were investigated in both human PBLs and sperm cells by alkaline comet assay.…”

Section: Cellular Genetic and Epigenetic Toxicities Of Svagsmentioning

confidence: 99%

General anesthesia, germ cells and the missing heritability of autism: an urgent need for research

Escher¹,

Ford²

2020

Environmental Epigenetics

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Agents of general anesthesia (GA) are commonly employed in surgical, dental and diagnostic procedures to effectuate global suppression of the nervous system, but in addition to somatic targets, the subject’s germ cells—from the embryonic primordial stage to the mature gametes—may likewise be exposed. Although GA is generally considered safe for most patients, evidence has accumulated that various compounds, in particular the synthetic volatile anesthetic gases (SVAGs) such as sevoflurane, can exert neurotoxic, genotoxic and epigenotoxic effects, with adverse consequences for cellular and genomic function in both somatic and germline cells. The purpose of this paper is to review the evidence demonstrating that GA, and in particular, SVAGs, may in some circumstances adversely impact the molecular program of germ cells, resulting in brain and behavioral pathology in the progeny born of the exposed cells. Further, we exhort the medical and scientific communities to undertake comprehensive experimental and epidemiological research programs to address this critical gap in risk assessment.

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“…Typically, several different drugs and techniques are combined during anesthesia [1]. Various methods have described DNA structure damage due to anesthesia [2][3][4][5], and thus there is evidence that anesthetics change human genetic information. Recent studies have been published concerning the genotoxicity of inhalational anesthesia in patients who have undergone surgery and in personnel who are occupationally exposed to anesthetics.…”

Section: Introductionmentioning

confidence: 99%

Does Neuraxial Anesthesia as General Anesthesia Damage DNA? A Pilot Study in Patients Undergoing Orthopedic Traumatological Surgery

Kuchařová

Astapenko

Zubáňová

et al. 2019

IJMS

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The human organism is exposed daily to many endogenous and exogenous substances that are the source of oxidative damage. Oxidative damage is one of the most frequent types of cell component damage, leading to oxidation of lipids, proteins, and the DNA molecule. The predominance of these damaging processes may later be responsible for human diseases such as cancer, neurodegenerative disease, or heart failure. Anesthetics undoubtedly belong to the group of substances harming DNA integrity. The goal of this pilot study is to evaluate the range of DNA damage by general and neuraxial spinal anesthesia in two groups of patients undergoing orthopedic traumatological surgery. Each group contained 20 patients, and blood samples were collected before and after anesthesia; the degree of DNA damage was evaluated by the comet assay method. Our results suggest that general anesthesia can cause statistically significant damage to the DNA of patients, whereas neuraxial anesthesia has no negative influence.

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Determination of DNA damage after exposure to inhalation anesthetics in human peripheral lymphocytes and sperm cells in vitro by comet assay

Cited by 24 publications

References 35 publications

Inhibition by Egb761 of the effect of cellphone radiation on the male reproductive system

Inhibition by Egb761 of the effect of cellphone radiation on the male reproductive system

General anesthesia, germ cells and the missing heritability of autism: an urgent need for research

Does Neuraxial Anesthesia as General Anesthesia Damage DNA? A Pilot Study in Patients Undergoing Orthopedic Traumatological Surgery

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