Determination of diclazuril, toltrazuril and its two metabolites in poultry tissues and eggs by gel permeation chromatography–liquid chromatography–tandem mass spectrometry

Ai, Lianfeng; Sun, Hanwen; Wang, Fengchi; Chen, Ruichun; Guo, Cheng

doi:10.1016/j.jchromb.2011.04.021

Cited by 27 publications

(13 citation statements)

References 11 publications

(24 reference statements)

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“…The detection limits of diclazuril were 0.005 mg/kg and mean recoveries (n = 5) of diclazuril in spiked chicken tissues were 92.0-102.0% (CV 5.9- 12.2%) (Shi, Zhu, Yun-Zhan, Ke-Zong, & Peng, 2009). LOD of diclazuril in real samples was 1.2 μg/kg in HPLC-MS/MS coupled to an electrospray ionization tandem mass spectrometer operating in the negative ion mode (Ai, Sun, Wang, Chen, & Guo, 2011). HPLC-MS/MS methods are rather time-consuming and require complicated sample preparation and expensive instruments.…”

Section: Assay Performance In Chicken Musclementioning

confidence: 99%

High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin–streptavidin system

Wang

et al. 2020

Food and Agricultural Immunology

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A simple, sensitive and specific chemiluminescence enzyme immunoassay based on biotin-streptavidin system (BASA-CLEIA) for diclazuril was established, which was about 2 and 4 times more sensitive than the traditional chemiluminescence enzyme immunoassay (CLEIA) and enzyme-linked immunosorbent assay. In the optimized generic assay, the half maximal inhibitory concentration (IC 50) of the BASA-CLEIA for diclazuril was 0.48 μg/kg. The limit of detection for diclazuril was 0.02 μg/kg. This method showed no cross reactivity (<0.01%) with toltrazuril, robenidine hydrochloride, nicarbazin, halofuginone, amprolium, monensin, and maduramycin. In chicken muscles, the average recoveries ranged from 90.3% to 120.4%. A further comparison between the developed BSAS-CLEIA and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for real samples showed good correlation (p < 0.01). The results indicated that the simple, sensitive and specific BASA-CLEIA method could be used for the analysis of diclazuril in real samples.

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Section: Assay Performance In Chicken Musclementioning

confidence: 99%

High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin–streptavidin system

Wang

et al. 2020

Food and Agricultural Immunology

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“…Toltrazuril may have clinical application in the treatment of Neospora caninum and other protozoal infections in cattle (Dirikolu et al, 2009;Martínez-Villalb et al, 2010). Toltrazuril is effective in vivo against Eimeria species in avians, in vitro against Toxoplasma gondii, and in vivo against intestinal and hepatic coccidiosis in rabbits (Peters and Geeroms,1986;Chapman,1987;Ricketts and Pfefferkorn,1993;Reynaud et al, 1999;Ai et al, 2011). However, due to toltrazuril's relatively poor water-soluble and low dissolution in gastric fluids, it is not well absorbed from the preparations.…”

Section: Toltrazurilmentioning

confidence: 99%

Preparation and characterization of toltrazuril polyethyleneglycol 6000 solid dispersions with improved solubility

Lu¹

2013

Afr. J. Pharm. Pharmacol.

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The current study investigated toltrazuril /polyethyleneglycol 6000 solid dispersions (toltrazuril/PEG6000 SDs) with improved solubility. The SDs were prepared by solvent-melting method with PEG 6000 as carrier. It was validated by differential thermal analysis (DTA) and cumulative dissolution rate. The solubility of toltrazuril, physical mixture and SDs were measured. The ultra violet (UV) spectrophotometer method was developed for the determination of toltrazuril. It was found that the spectra of cumulative dissolution rate, DTA of the SDs were different from the toltrazuril and physical mixture. Solubility of toltrazuril was enhanced for the formation of SDs. The calibration curve was linear with a correlation coefficient r = 0.9998 in range of 4.0～20.0 µg/mL. The method was simple and practical in preparation and determination the toltrazuril SDs.

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“…At the moment, there are few papers about toltrazuril and its metabolites in eggs or in feedstuffs [30][31][32] and, to our knowledge, there are no scientific works about these five synthetic coccidiostats and the toltrazuril metabolites all together.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatography Tandem Mass Spectrometry Analysis of Synthetic Coccidiostats in Eggs

et al. 2017

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Abstract:Coccidiostats are synthetic drugs administered to animals, especially to poultry, to cure coccidiosis. In this paper, we present a selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to analyze residues of five synthetic coccidiostats in eggs: clazuril, diclazuril, robenidine, nicarbazin, toltrazuril and its two metabolites. The extraction efficiency was evaluated by testing several solvents, pH, different volumes and time of extraction. The clean-up procedures were optimized using different solid phase extraction cartridges and different eluants. The chromatographic separation was achieved in reversed phase using a gradient of 0.1% formic acid in water and acetonitrile, whereas the MS detection was performed in negative electrospray ionization (ESI) for all the analytes, except for the robenidine. The developed method has been validated according to Commission Decision 2002/657/CE. The validation parameters, as linearity, precision, recovery, specificity, decision limit (CCα), detection capability (CCβ), and robustness have been determined. The proposed method resulted simple, fast, and suitable for screening and confirmation purposes.

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Determination of diclazuril, toltrazuril and its two metabolites in poultry tissues and eggs by gel permeation chromatography–liquid chromatography–tandem mass spectrometry

Cited by 27 publications

References 11 publications

High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin–streptavidin system

High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin–streptavidin system

Preparation and characterization of toltrazuril polyethyleneglycol 6000 solid dispersions with improved solubility

Liquid Chromatography Tandem Mass Spectrometry Analysis of Synthetic Coccidiostats in Eggs

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