Determination of deoxynivalenol (DON) and its derivatives: Current status of analytical methods

Rapid prediction of deoxynivalenol contamination in wheat bran by MOS‐based electronic nose and characterization of the relevant pattern of volatile compounds

“…For these reasons, several immunoassays, including enzyme‐linked immunosorbent assay (ELISA), flow‐through immunoassays (FIA), lateral flow devices (LFD), and fluorescence polarization immunoassays (FPIA), have been extensively developed for the rapid screening of DON in cereals due to their rapidity, simplicity, and cost effectiveness . In particular, an FPIA for the determination of DON in wheat bran and whole‐wheat flour showed performances, in terms of sensitivity, accuracy, and precision, comparable to those of chromatographic techniques .…”

Occurrence of Fusarium mycotoxins and their dietary intake through beer consumption by the European population

“…GC offers some benefits over LC methods such as lower instrument cost as well as lower maintenance, even though analysis of mycotoxins by GC requires derivatization. Ion sources used in LC-MS are also related to ion suppression or enhancement with strong consequences on the accuracy, precision and sensitivity of the methods (Ran et al, 2013).…”

Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation

“…3-ADON, 15-ADON and T-2 showed 100% of deacetylation after 24 h of exposure. The Michaelis-Menten (K M ) constant, which shows the affinity of an enzyme for its substrate, revealed that carboxylesterase had a major affinity to 15 The data presented from our work represents a preliminary study, which demonstrated that optimization can be used to provide even more promising degradation values. The use of enzymes with high purification grade and low cost, presented in this work, represents a significant advance to the works mentioned so far, in terms of velocity, time and selectivity in enzymatic action specificity.…”

“…For trichothecenes, derivatization is performed by silanization, acylation and alkylation reactions. 11,15 The development of a method suitable for multiple mycotoxins, with a common sample preparation procedure, requiring only a final determination is highly desirable. Most analytical techniques used for the determination of trichothecenes proposed their extraction from the food matrix into a solvent.…”