Determination of DEHP in blood products stored in plastic bags by HPLC

Gerlai, I.; Pintér, J.; Pick, John

doi:10.1007/bf02688515

Cited by 8 publications

(4 citation statements)

References 5 publications

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“…On day 4 of storage the lowest pH value in PL 146 bags was 6.12, therefore longer storage does not seem to be advisable. DEHP concentration found in PCs stored in different bags are in good agreement with data obtained by other investigators [12,[17][18][19][20]. When mean values…”

supporting

confidence: 82%

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Effect of DEHP Plasticizer on Stored Platelets

Rácz¹,

Baróti²

1995

Vox Sanguinis

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supporting

confidence: 82%

“…The DEHP content of the supernatant was determined by high-performance liquid chromatography [12]. Platelet aggregation was studied at 37°C with 50 \iM adenosine-diphosphate (ADP); hypotonic shock response was determined as described earlier [13].…”

mentioning

confidence: 99%

Effect of DEHP Plasticizer on Stored Platelets

Rácz¹,

Baróti²

1995

Vox Sanguinis

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“…The rate of accumulation of DEHP was rapid for the first week, after which time there was an approximate equilibrium established. In the literature, several results were different because the storage conditions were different, for example at 4 "C and without shaking (Gerlai et al, 1987;Shintani, 1985). However, Rock et by steam (121"C), ethylene oxide (60°C) or yirradiation (room temperature).…”

Section: Dehp In Ppp Stored Under Particular Conditionsmentioning

confidence: 95%

Rapid determination by high performance liquid chromatography of di‐2‐ethylhexyl phthalate in plasma stored in plastic bags

Dine¹,

Luyckx²,

Cazin³

et al. 1991

Biomedical Chromatography

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A rapid sensitive technique was developed for the analysis of di-2-ethylhexyl phthalate (DEHP) in plasma stored in plastic bags by using high performance liquid chromatography (HPLC) with UV detection and a Hypersil ODS column. The compound was easily and efficiently extracted with a mixture of sodium hydroxide and acetonitrile, which allowed the deproteinization of plasma samples. The recovery was greater than 95% and the intra- and inter-assay coefficients of variation were better than 6.5%. The results obtained showed that the amount of DEHP accumulated in plasma varied according to different parameters and depended on the storage conditions (time, temperature and shaking) and also on the lipid content of the stored plasma and the sterilization process of the PVC bags.

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“…mannitol) due to precipitation of osmogens [10][11] . Other methods using hexane and ethyl acetate as mobile phase components to monitor DEHP and its metabolites in plasma are cumbersome as these methods require lengthy and time consuming solvent-solvent extraction of aqueous samples before analysis 12 . A variety of sophisticated and expensive analytical methods are available that utilize techniques like LCMS, GC, GC-MS, electron capture GC [13][14][15] or involve micro-organic ion association phase extraction 16 to determine DEHP and metabolites in plasma or samples from variety of matrices.…”

Section: Introductionmentioning

confidence: 99%

Validated Reverse Phase HPLC Method for the Determination of DEHP Content in Reconstituting Diluents and in Reconstituted Solutions of Imipenem and Cilastatin for Injection

Chaudhary¹,

Waske²,

Yadav³

et al. 2009

Journal of Chemistry

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A simple, rapid, accurate and economic reverse phase HPLC method was developed and validated for determination of di-(2-ethylhexyl)phthalate (DEHP) in reconstituting diluents and reconstituted solutions of imipenem and cilastatin for injection The method was applied to detect any leaching enhancement in presence of imipenem and cilastatin in reconstituted injections. Imipenem and cilastatin for injection is a widely used broad spectrum beta-lactam antibiotic. As per requirements of various regulatory authorities of different countries, DEHP content needs to be monitored in the reconstituting diluents used to reconstitute injections. The proposed method is a unique method wherein DEHP can be determined directly without any sample pre-treatment before analysis. The method can be used as a good quality control tool to control the leaching of DEHP in the reconstituting diluents and reconstituted injections. The method utilizes C18column (250 × 4.6 mm, 5μ) and a mixture of methanol, propan-2-ol and water as mobile phase. DEHP was detected at 225 nm. The method has low limit of quantification (0.058 μg mL-1) which is much below the acceptance limit calculated as per USFDA tolerance criteria (3.5 μg mL-1for adults and 0.3 μg mL-1for neonates and infants).

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Determination of DEHP in blood products stored in plastic bags by HPLC

Cited by 8 publications

References 5 publications

Effect of DEHP Plasticizer on Stored Platelets

Effect of DEHP Plasticizer on Stored Platelets

Rapid determination by high performance liquid chromatography of di‐2‐ethylhexyl phthalate in plasma stored in plastic bags

Validated Reverse Phase HPLC Method for the Determination of DEHP Content in Reconstituting Diluents and in Reconstituted Solutions of Imipenem and Cilastatin for Injection

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