Determination of cytotoxicity following oxidative treatment of pharmaceutical residues in wastewater

Graumans, Martien H.F.; Hove, Hedwig van; Schirris, Tom J.J.; Hoeben, Wflm Wilfred; Dael, Maurice van; Anzion, Rob; Rüssel, Frans G. M.; Scheepers, Paul T.J.

doi:10.1016/j.chemosphere.2022.135022

Cited by 7 publications

(8 citation statements)

References 54 publications

(111 reference statements)

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“…Molecular structures needed for the in silico predictions were retrieved from our optimization experiment Graumans et al (2020), degradation study Graumans et al (2021) and cytotoxicity analysis Graumans et al (2022). For this in silico study, a versatile set of 10 pharmaceuticals formerly detected in hospital sewage water were used as model compounds.…”

Section: Methodsmentioning

confidence: 99%

“…An example of a bacterium endotoxin contributing to the wastewater toxicity might be lipopolysaccharide (LPS). In our previous study it was found that LPS affects the viability of HeLa cells (>50% cell death) when present at high concentrations (10-1000 μg/L) (Graumans et al, 2022). Immediate cell death was found when HeLa cells were exposed to untreated HSW, which be reversed by thermal plasma and UV-C/H 2 O 2 oxidation treatment for prolonged treatment times (Graumans et al, 2022).…”

Section: Thermal Plasma Treatmentmentioning

confidence: 99%

“…In vitro toxicity testing is considered suitable to evaluate efficacy by comparing the cytotoxicity of wastewater before and after treatment with plasma oxidation (Graumans et al, 2022). In our previous studies, we used thermal plasma activation for the oxidative degradation of pharmaceutical residues (Graumans et al, 2020(Graumans et al, , 2021.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, a cytotoxicity assay was applied to determine the harmfulness of hospital sewage water before and after oxidative treatment in a human cell culture. This resulted in minimal toxicity after direct thermal plasma treatment (Graumans et al, 2022). Due to the complexity of wastewater it can be difficult to attribute an observed effect to original products or its corresponding transformation product formed during human metabolism, microbial biotransformation or oxidative conversion (Drzymała and Kalka, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In Silico Ecotoxicity Assessment of Pharmaceutical Residues in Wastewater Following Oxidative Treatment

Graumans,

Hoeben,

Ragas

et al. 2023

Preprint

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Section: Methodsmentioning

confidence: 99%

Section: Thermal Plasma Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In Silico Ecotoxicity Assessment of Pharmaceutical Residues in Wastewater Following Oxidative Treatment

Graumans,

Hoeben,

Ragas

et al. 2023

Preprint

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“…Moreover, the degradation of pharmaceutical waste using conventional methods such as biological and chemical treatment is very difficult (2). Therefore, techniques like advanced oxidation processes (AOPs) have been gaining substantial attention in pharmaceutical wastewater degradation in the modern world (4,(7)(8)(9)(10)(11)(12). Furthermore, plasma technology is an emerging technology in the field of pharmaceutical wastewater degradation.…”

Section: Introductionmentioning

confidence: 99%

Penicillin Antibiotic (Ampicillin and Cloxacillin) Degradation Using Non-Thermal Pencil Plasma Jet

Rathore

2023

Preprint

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In the present work an antibiotic drug degradation efficacy of a non-thermal pencil plasma jet in artificial wastewater. Air is used as a plasma-forming gas. The air plasma is produced in a cylindrical co-axial assembly of electrodes powered by a 50 Hz High-voltage power supply. The electrical characterization of plasma is performed and the generated plasma species/radicals are identified using optical emission spectroscopy. The degradation of antibiotics in solution after plasma treatment is analyzed using high performance liquid chromatography with photodiode array detector (HPLC-PDA). In addition, total organic carbon (TOC), and chemical oxygen demand (COD) is also determined. The HPLC-PDA results reveal that plasma degradation of antibiotics solution followed first-order degradation kinetics with a rate constant of 0.158 min-1 (ampicillin) and 0.366 min-1 (Cloxacillin). Furthermore, the reduction in TOC and COD supported the degradation of antibiotics using plasma jet. It has been observed the plasma 9-minute gives 76.10% and 99.99% degradation in ampicillin and cloxacillin solution respectively. The change in physicochemical properties and various reactive species formed due to which degradation of antibiotics solution take place are measured. Finally, the toxicity of plasma degraded antibiotic solutions are studied on freshwater chlorella algae species. The results reveal that plasma degraded antibiotics solutions are non-toxic toward freshwater algae. The non-thermal plasma jet could be a potential eco-friendly alternative for the degradation of various antibiotic drugs present in wastewater.

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Methotrexate degradation in artificial wastewater using non-thermal pencil plasma jet

Rathore

Patel

Pandey

et al. 2023

Environ Sci Pollut Res

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The rising global cancer rate is driving up the consumption of anticancer drugs. This causing a noticeable increase in the levels of these drugs in wastewater. The drugs are not metabolized effectively by the human body, leading to their presence in human waste, as well as in the 2 effluent from hospitals and drug manufacturing industries. Methotrexate is a commonly used drug for treating various types of cancer. Its complex organic structure makes it difficult to degrade using conventional methods. The present work proposed a non-thermal pencil plasma jet treatment for methotrexate degradation. The air plasma produced in this jet setup is electrical characterized and plasma species/radicals are identified using emission spectroscopy. The degradation of drug is monitored by studying the change in solution physiochemical properties, HPLC-UV analysis, and removal of total organic carbon, etc.Results show that a 9-minute plasma treatment completely degraded the drug solution that followed first order degradation kinetics with rate constant 0.38 min -1 and 84.54% mineralization was observed. Additionally, an increase in electrical conductivity and dissolved solids compared to virgin water-plasma interaction indicated the formation of new, smaller compounds (2,4-Diaminopteridine-6-carboxylic acid, N-(4-Aminobenzoyl)-L-glutamic acid, etc.) after drug degradation. The plasma-treated methotrexate solution also showed lower toxicity towards freshwater chlorella algae compared to the untreated solution. Finally, it can be said that non-thermal plasma jets are economically and environmentally friendly devices that have the potential to be used for the treatment of complex and resistive anticancer drugpolluted wastewaters.

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Determination of cytotoxicity following oxidative treatment of pharmaceutical residues in wastewater

Cited by 7 publications

References 54 publications

In Silico Ecotoxicity Assessment of Pharmaceutical Residues in Wastewater Following Oxidative Treatment

In Silico Ecotoxicity Assessment of Pharmaceutical Residues in Wastewater Following Oxidative Treatment

Penicillin Antibiotic (Ampicillin and Cloxacillin) Degradation Using Non-Thermal Pencil Plasma Jet

Methotrexate degradation in artificial wastewater using non-thermal pencil plasma jet

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