Determination of Cystatin C in human serum by isotope dilution mass spectrometry using mass overlapping peptides

González-Antuña, Ana; Rodríguez-González, Pablo; Ohlendorf, Rüdiger; Henrion, André; Delatour, Vincent; Alonso, J. Ignacio García

doi:10.1016/j.jprot.2014.09.005

Cited by 30 publications

(24 citation statements)

References 32 publications

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“…The situation is less evident for Jaffe assays, even when manufacturers claim that traceability has been obtained [30;31]. Due to its molecular structure, the measurement of cysC by mass spectrometry is much more complex even if such a method has been recently made available [16]. However, to the best of our knowledge, this method has not yet been used to determine the "true value" of the cysC content of the reference material ERM-DA471/IFCC proposed by the IFFC working group that manufacturers should use to calibrate their assays [17; 18].…”

Section: Discussionmentioning

confidence: 99%

“…At present the majority of creatinine enzymatic assays are standardized to mass spectrometry and can be considered IDMS (isotope dilution mass spectrometry)-traceable [15]. To achieve the same level of standardization as for creatinine, an international standard and a reference method for accurate quantification of cysC in the standard material [16] are mandatory. An important step in the standardization of cysC assays was achieved in 2010 when the International Federation for Clinical Chemistry and Laboratory Medicine (IFCC) working group made the international certified reference material ERM-DA471/IFCC available to manufactures [17; 18].…”

Section: Introductionmentioning

confidence: 99%

“…An important step in the standardization of cysC assays was achieved in 2010 when the International Federation for Clinical Chemistry and Laboratory Medicine (IFCC) working group made the international certified reference material ERM-DA471/IFCC available to manufactures [17; 18]. Also, an ID/LSMS method has been recently published for cysC [16] which would allow the determination of the exact concentration of cysC in the content of the IFCC standard. However, in recent papers it has been shown that the use of such material has been inconsistent, leading to doubt about the accuracy of several cysC assays on the market: Grubb et al have shown that the variability of different cysC assays could be reduced [19], but these optimistic results have not yet been confirmed by others [13; 19-22].…”

Section: Introductionmentioning

confidence: 99%

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Cystatin C standardization decreases assay variation and improves assessment of glomerular filtration rate

Ebert

Delanaye

Shlipak

et al. 2016

Clinica Chimica Acta

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Background: Cystatin C is increasingly used in glomerular filtration rate (GFR) estimation equations. The dependence of cystatin C results upon the analytical method has been a major source of controversy.Methods: Cystatin C was measured with non-standardized turbidimetric Roche Generation 1 and standardized nephelometric Siemens assays in 3666 and additionally with standardized Roche Generation 2 and Siemens in 567 blood samples of the Berlin Initiative Study. Cystatin C-based GFR was assessed with CKD-EPIcys (Chronic Kidney Disease Epidemiology) and CAPA (Caucasian, Asian, Pediatric, Adult) equations and the impact of the assays on GFR estimation was determined. Equation performance compared to measured GFR was evaluated.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cystatin C standardization decreases assay variation and improves assessment of glomerular filtration rate

Ebert

Delanaye

Shlipak

et al. 2016

Clinica Chimica Acta

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“…However, these 2 markers have their own variability. For cystatin C, large efforts both from experts and manufacturers have led to a better standardization of the measurement (with the development of an international certified reference material ERM-DA471/ IFCC provided by the International Federation for Clinical Chemistry and Laboratory Medicine to manufacturers [61] or the development of mass spectrometry method to measure cystatin C [62,63] ) [63,64] . Such standardization between assays does not exist for beta-trace protein.…”

Section: How Could We Still Decrease the Variability In Egfr?mentioning

confidence: 99%

Serum Creatinine: Not So Simple!

Delanaye

Pottel²

2017

Nephron

244

171

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Measuring serum creatinine is cheap and commonly done in daily practice. However, interpretation of serum creatinine results is not always easy. In this review, we will briefly remind the physiological limitations of serum creatinine due notably to its tubular secretion and the influence of muscular mass or protein intake on its concentration. We mainly focus on the analytical limitations of serum creatinine, insisting on important concept such as reference intervals, standardization (and IDMS traceability), analytical interferences, analytical coefficient of variation (CV), biological CV and critical difference. Because the relationship between serum creatinine and glomerular filtration rate is hyperbolic, all these CVs will impact not only the precision of serum creatinine but still more the precision of different creatinine-based equations, especially in low or normal-low creatinine levels (or high or normal-high glomerular filtration rate range).

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“…In this way, we can accurately measure the real isotopic distribution of incell molecular fragments with a small number of SRM transitions, as described in a previous publication. 30 The protonated molecular ion [M+H + ] was selected as precursor ion for both compounds. The acquired SRM transitions for creatine were 132 → 90, 132 → 91, 132 → 92, 132 → 93; for creatinine, the SRM transitions were 114 → 86, 114 → 87, 114 → 88, 114 → 89.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Simultaneous Determination of Creatinine and Creatine in Human Serum by Double-Spike Isotope Dilution Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS) and Gas Chromatography–Mass Spectrometry (GC-MS)

et al. 2015

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This work describes the first multiple spiking isotope dilution procedure for organic compounds using (13)C labeling. A double-spiking isotope dilution method capable of correcting and quantifying the creatine-creatinine interconversion occurring during the analytical determination of both compounds in human serum is presented. The determination of serum creatinine may be affected by the interconversion between creatine and creatinine during sample preparation or by inefficient chemical separation of those compounds by solid phase extraction (SPE). The methodology is based on the use differently labeled (13)C analogues ((13)C1-creatinine and (13)C2-creatine), the measurement of the isotopic distribution of creatine and creatinine by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the application of multiple linear regression. Five different lyophilized serum-based controls and two certified human serum reference materials (ERM-DA252a and ERM-DA253a) were analyzed to evaluate the accuracy and precision of the proposed double-spike LC-MS/MS method. The methodology was applied to study the creatine-creatinine interconversion during LC-MS/MS and gas chromatography-mass spectrometry (GC-MS) analyses and the separation efficiency of the SPE step required in the traditional gas chromatography-isotope dilution mass spectrometry (GC-IDMS) reference methods employed for the determination of serum creatinine. The analysis of real serum samples by GC-MS showed that creatine-creatinine separation by SPE can be a nonquantitative step that may induce creatinine overestimations up to 28% in samples containing high amounts of creatine. Also, a detectable conversion of creatine into creatinine was observed during sample preparation for LC-MS/MS. The developed double-spike LC-MS/MS improves the current state of the art for the determination of creatinine in human serum by isotope dilution mass spectrometry (IDMS), because corrections are made for all the possible errors derived from the sample preparation step.

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Determination of Cystatin C in human serum by isotope dilution mass spectrometry using mass overlapping peptides

Cited by 30 publications

References 32 publications

Cystatin C standardization decreases assay variation and improves assessment of glomerular filtration rate

Cystatin C standardization decreases assay variation and improves assessment of glomerular filtration rate

Serum Creatinine: Not So Simple!

Simultaneous Determination of Creatinine and Creatine in Human Serum by Double-Spike Isotope Dilution Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS) and Gas Chromatography–Mass Spectrometry (GC-MS)

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