Determination of cyprodinil and fludioxonil in the fermentative process of must by high‐performance liquid chromatography–diode array detection

Abstract

BACKGROUND: A quantitative, selective and sensitive high-performance liquid chromatographic method is described for the analysis of new fungicides cyprodinil, fludioxonil and their commercial formulation Switch in model solutions of must and wine, as well as samples during alcoholic fermentation. A study of the dissipation of residues was carried out.

Moiety and linker site heterologies for highly sensitive immunoanalysis of cyprodinil in fermented alcoholic drinks

“…Several methods have been described for the detection of cyprodinil traces in wines, based on gas or liquid chromatography with mass spectrometry detection. A previous extraction step is usually employed to avoid matrix effects, such as solid-phase microextraction with divinylbenzeneecarboxenepolydimethylsiloxane fibres (Rial-Otero, Yagüe-Ruiz, Cancho-Grande, & Simal-G andara, 2002), liquideliquid extraction (Vaquero-Fern andez et al, 2008), solid-phase extraction with Oasis HLB cartridges (Fontana, Rodríguez, Ramil, Altamirano, & Cela, 2011), dispersive liquideliquid microextraction (Rodríguez-Cabo, Rodríguez, Ramil, & Cela, 2011), or the QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure (Moeder, Bauer, Popp, van Pinxteren, & Reemtsma, 2012;Walorczyk, Drozdzynski, & Gnusowski, 2011). Also, immunoassays have been developed and validated in wines for the analysis of pesticides like benalaxyl (Rosso, Giraudi, Gamberini, Baggiani, & Vanni, 2000), tebufenozide (Irwin, Tolhurst, Jackson, & Gale, 2003), fenhexamid (Mercader & Abad-Fuentes, 2009), or bromopropylate (Ramon-Azc on, S anchez-Baeza, Sanvicens, & Marco, 2009), showing excellent performance.…”

Voltammetric and corrosion studies of the fungicide fludioxonil

“…The mode of action of Flu is the inhibition of the transport-associated phosphorylation of glucose, as well as the prevention of glycerol synthesis [2,3]. Flu can be applied directly to control seeds, and also as a post-harvest treatment of fruit (stone fruit, pome fruit, pomegranate, kiwifruit, and citrus) [4].…”

“…Because Flu can have harmful effects on living organisms, it is important to elaborate analytical methods for its determination within the lowest possible concentration range. Therefore, different chromatographic techniques have been used, and the most common among them are high performance liquid chromatography coupled with a diode-array detector (HPLC-DAD) [2], gas chromatography coupled with nitrogen-phosphorus (GC-NPD) [9] and mass spectrometry (GC-MS) detectors [3,9], and liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) [10]. Reversed migration micellar electrokinetic chromatography (RM-MEKC) [11] is recognized as another powerful analytical technique for the determination of Flu.…”

Development of a liquid–liquid extraction method for the determination of pyrimethanil, metalaxyl, dichlofluanid and penconazol during the fermentative process of must by GC‐NPD

“…However, the most commonly used treatment method for liquid samples is liquid–liquid extraction (LLE) with organic solvents. Acetonitrile, hexane, dichloromethane or ethyl acetate are the most used 15–20…”