Determination of creep compliance, recovery and Poisson's ratio of elastomers by means of digital image correlation (DIC)

Farfán-Cabrera, Leonardo Israel; Pascual-Francisco, Juan Benito; Barragán-Pérez, Omar; Gallardo-Hernández, Ezequiel Alberto; Susarrey-Huerta, Orlando

doi:10.1016/j.polymertesting.2017.02.010

Cited by 36 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the creep response was measured, the force was removed, and the subsequent relaxation of the cells was recorded for another 2 s. In order to retrieve cell mechanical properties from the recorded displacement curves, we calculated the compliance J(t). The creep compliance is defined as the ratio of strain ε over stress σ and is a commonly used property to describe the mechanical properties of viscoelastic materials 33 , 45 , 46 . The sheer stress experienced by the cell is equal to the ratio of the applied force ΔF and the contact area A between cell and bead.…”

Section: Methodsmentioning

confidence: 99%

Cell mechanical properties of human breast carcinoma cells depend on temperature

Aermes

Hayn

Fischer

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The knowledge of cell mechanics is required to understand cellular processes and functions, such as the movement of cells, and the development of tissue engineering in cancer therapy. Cell mechanical properties depend on a variety of factors, such as cellular environments, and may also rely on external factors, such as the ambient temperature. The impact of temperature on cell mechanics is not clearly understood. To explore the effect of temperature on cell mechanics, we employed magnetic tweezers to apply a force of 1 nN to 4.5 µm superparamagnetic beads. The beads were coated with fibronectin and coupled to human epithelial breast cancer cells, in particular MCF-7 and MDA-MB-231 cells. Cells were measured in a temperature range between 25 and 45 °C. The creep response of both cell types followed a weak power law. At all temperatures, the MDA-MB-231 cells were pronouncedly softer compared to the MCF-7 cells, whereas their fluidity was increased. However, with increasing temperature, the cells became significantly softer and more fluid. Since mechanical properties are manifested in the cell’s cytoskeletal structure and the paramagnetic beads are coupled through cell surface receptors linked to cytoskeletal structures, such as actin and myosin filaments as well as microtubules, the cells were probed with pharmacological drugs impacting the actin filament polymerization, such as Latrunculin A, the myosin filaments, such as Blebbistatin, and the microtubules, such as Demecolcine, during the magnetic tweezer measurements in the specific temperature range. Irrespective of pharmacological interventions, the creep response of cells followed a weak power law at all temperatures. Inhibition of the actin polymerization resulted in increased softness in both cell types and decreased fluidity exclusively in MDA-MB-231 cells. Blebbistatin had an effect on the compliance of MDA-MB-231 cells at lower temperatures, which was minor on the compliance MCF-7 cells. Microtubule inhibition affected the fluidity of MCF-7 cells but did not have a significant effect on the compliance of MCF-7 and MDA-MB-231 cells. In summary, with increasing temperature, the cells became significant softer with specific differences between the investigated drugs and cell lines.

show abstract

Section: Methodsmentioning

confidence: 99%

Cell mechanical properties of human breast carcinoma cells depend on temperature

Aermes

Hayn

Fischer

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The possibilities of using this method are very wide due to the fact that it is able to measure small displacements in micro and nano scale and also measure great displacements of diverse dimensions. The DIC technique has been used in both studies of hyperelastic polymers [56]- [58] and in the analysis of the mechanical behavior of various materials used in industries [59], [60].…”

Section: Digital Image Correlationmentioning

confidence: 99%

Study of PDMS characterization and its applications in biomedicine: A review

Victor¹,

Ribeiro²,

Araújo³

2019

JMEB

114

View full text Add to dashboard Cite

______________________________________________________________________ AbstractPolydimethylsiloxane, also known as PDMS, has been widely used in the biomedical industry because of its biocompatible material and its biomechanical behavior is similar to biological tissues, with applications in the study of aneurysmal behavior and devices such as: Micro pumps, optical systems, microfluidic circuits. Many advances in research have been reached, but further tests are still necessary to understand the mechanical behavior and applicability of the material. For the study of PDMS behavior, two different techniques are employed: numerical and experimental. In experimental studies, it is extremely popular to use a field technique, in which the most appropriate technique to measure the field displacement of PDMS, without decorrelation, is the Digital Image Correlation (DIC) method. In this paper, we revised the most important experimental works with PDMS material Review Article

show abstract

“…In terms of compression dilatometry testing, deformations can be induced by hydrostatic or piston compression [1]. In uniaxial loading conditions, the determination of the Poisson's ratio is often approached by creep and cyclic loading tests [2][3][4]. Changes in the volume of elastomers can be observed as a function of time, temperature, and cyclic loading [5], either compressive or tensile.…”

Section: Introductionmentioning

confidence: 99%

Volume changes measurement of elastomers using 3D DIC

Kotlánová,

Rusnáková,

Ogunleye

et al. 2023

APP

View full text Add to dashboard Cite

The presented research aims at measuring the volume changes of elastomers using digital image correlation (DIC) in the 3D configuration (stereo DIC). The deformation measurement method in stereo configuration was applied during the mechanical testing of two types of test specimens (dumbbells, cylinders) in uniaxial tension. In this configuration, two cameras were used. The test specimens were measured up to a specified strain value, and the measured data were used to obtain the strain dependence of Poisson’s ratio and the bulk modulus, which are crucial for hyperelastic models. The measurement results reveal that the stereo DIC method provides relatively less scattered data in the low-strain regions for dumbbell-shaped test specimens and is suitable for measuring various test specimen shapes.

show abstract

Determination of creep compliance, recovery and Poisson's ratio of elastomers by means of digital image correlation (DIC)

Cited by 36 publications

References 15 publications

Cell mechanical properties of human breast carcinoma cells depend on temperature

Cell mechanical properties of human breast carcinoma cells depend on temperature

Study of PDMS characterization and its applications in biomedicine: A review

Volume changes measurement of elastomers using 3D DIC

Contact Info

Product

Resources

About