Determination of Conspecificity ofCandida UtilisandHansenula JadiniiThrough DNA Reassociation

A history of research on yeasts 8: taxonomy

“…In 1970, Wickerham had drawn attention to the phenotypic similarities between C. utilis and Hansenula jadinii, an ascospore-producing species ( [265] p. 285). Nine years later, Cletus Kurtzman and his colleagues measured DNA reassociation 50 between three strains of C. utilis and one of H. jadinii and, finding that they had 85% base sequences in common, Kurtzman concluded that C. utilis is the asexual state of H. jadinii [141]. This conclusion was based on the view that strains with >80% DNA base sequences in common should be treated as the same species [204].…”

Bacillus thermodenitrificans sp. nov., nom. rev.

“…DNA was isolated and purified as described previously (Manachini et al, 1985). DNA-DNA homology was determined by the optical (Kurtzman et al, 1979) Plasmid detection. Detection of plasmid DNA followed the alkaline extraction procedure described by Sambrook et al (1989).…”

Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov.

“…DNA was isolated and purified as described previously (Manachini et al, 1985). DNA-DNA homology was determined by the optical renaturation rates method (Kurtzman et al, 1979) with a model Response spectrophotometer equipped with Advance Kinetics Graphic Version 1n3 thermoprogrammer (Gilford System ; Ciba Corning Diagnostic Corp.). For all samples tested the melting temperature (T m ) was calculated in 5i SSC, the same salt concentration used for the determination of DNA-DNA similarities.…”