Determination of Codeine Phosphate in the Culture Fluid of Rhodococcus by High-Performance Liquid Chromatography

Plotnikov, A.N.; Karpenko, Yu. N.; Vikhareva, E. V.; Tyumina, Elena A.; Richkova, M. I.; Selyaninov, A. A.

doi:10.3103/s0027131418010054

Cited by 11 publications

(4 citation statements)

References 14 publications

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“…Among the microorganisms, which carry out the processes of natural attenuation of anthropogenic xenobiotics are actinobacteria, typical inhabitants of aquatic and soil ecosystems, with the greatest variety of degradable pollutants and a wide range of adaptive capabilities [9][10][11]. Effective actinobacterial degradation of selected antibiotics, hormones, antiepileptics, analgesics, and nonsteroidal anti-inflammatory drugs (NSAIDs) has already been documented [12][13][14][15][16][17]. Earlier, we confirmed the ability of rhodococci to completely biodegrade pharmaceuticals from analgesics and antispasmodics, including paracetamol [18], drotaverine hydrochloride [19,20], and diclofenac sodium [21,22].…”

Section: Introductionmentioning

confidence: 99%

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

et al. 2021

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The article expands our knowledge on the variety of biodegraders of ibuprofen, one of the most frequently detected non-steroidal anti-inflammatory drugs in the environment. We studied the dynamics of ibuprofen decomposition and its relationship with the physiological status of bacteria and with additional carbon and energy sources. The involvement of cytoplasmic enzymes in ibuprofen biodegradation was confirmed. Within the tested actinobacteria, Rhodococcus cerastii IEGM 1278 was capable of complete oxidation of 100 μg/L and 100 mg/L of ibuprofen in 30 h and 144 h, respectively, in the presence of an alternative carbon source (n-hexadecane). Besides, the presence of ibuprofen induced a transition of rhodococci from single- to multicellular lifeforms, a shift to more negative zeta potential values, and a decrease in the membrane permeability. The initial steps of ibuprofen biotransformation by R. cerastii IEGM 1278 involved the formation of hydroxylated and decarboxylated derivatives with higher phytotoxicity than the parent compound (ibuprofen). The data obtained indicate potential threats of this pharmaceutical pollutant and its metabolites to biota and natural ecosystems.

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Section: Introductionmentioning

confidence: 99%

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

et al. 2021

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“…HPLC has been reported to simultaneously detect several opioid drugs with similar structures such as heroin, morphine, and codeine in the sample due to its ability to separate the drugs [ 73 ]. Various detectors have been coupled with HPLC including optical detectors (e.g., photomultiplier tube (PMT) and photodiode array (PDA)) [ 76 , 77 ] and mass spectrometry (MS) [ 78 , 79 , 80 , 81 , 82 ].…”

Section: Chromatographic Analysismentioning

confidence: 99%

A Review of Analytical Methods for Codeine Determination

et al. 2021

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Codeine is derived from morphine, an opioid analgesic, and has weaker analgesic and sedative effects than the parent molecule. This weak opioid is commonly used in combination with other drugs for over-the-counter cough relief medication. Due to the psychoactive properties of opioid drugs, the easily obtained codeine often becomes subject to misuse. Codeine misuse has emerged as a concerning public health issue due to its associated adverse effects such as headache, nausea, vomiting, and hemorrhage. Thus, it is very important to develop reliable analytical techniques to detect codeine for both quality control of pharmaceutical formulations and identifying drug misuse in the community. This review aims to provide critical outlooks on analytical methods applicable to the determination of codeine.

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“…Динамика убыли количества вещества в процессе биодеструкции регистрировалась с помощью метода высокоэффективной жидкостной хроматографии (ВЭЖХ). При этом проводился подбор условий хроматографического определения фармвещества в специфической биожидкости -среде культивирования бактерий [Karpenko et al, 2014;Plotnikov et al, 2017;Khrenkov et al, 2020;Vikhareva et al, 2023].…”

Section: Introductionunclassified

Enhancing the biodegradation of ibuprofen through factor analysis and kinetic modeling

Bazhutin,

Tyumina,

Selyaninov

et al. 2023

Bulletin of Perm University. Biology

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Ibuprofen ranks as one of the most frequently detected pharmaceutical pollutants in water across many countries. Previous studies identified conditions for actinobacterial degradation of ibuprofen and developed a chromatographic analysis technique, enabling the measurement of residual ibuprofen levels during bio-degradation. However, the impact of a complex interplay of factors on the dynamics of ibuprofen biodegradation remains insufficiently explored. The objective of this study was to enhance the biodegradation process of ibu-profen through a multifactorial experiment that controled key parameters and to predict changes in substance content and the time needed for process optimization using kinetic modeling. As a result of this study, varying the concentrations of glucose, n-hexadecane, inoculum, and pH values has enabled a remarkable reduction in the biodegradation process duration for ibuprofen (0.1 g/L), from 10 days to just 2 days when employing cosub-strates in quantities of 1.0 g/L, 10.0 ml/l, and adhering to the turbidity standard NTU-5, along with pH stabiliza-tion at 6.5. By applying the kinetic equation of the first-order derivative, dx/dt = –k x-1, we determined the pa-rameter ‘k’ for the ibuprofen biodegradation rate to be 138.88÷146.84%2/h. The half-life (t1/2) was calculated as 25.54÷27.00 hours, and the process’s endpoint, t1/100, was refined to 34.05÷36.00 hours under the optimized conditions.

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Determination of Codeine Phosphate in the Culture Fluid of Rhodococcus by High-Performance Liquid Chromatography

Cited by 11 publications

References 14 publications

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

A Review of Analytical Methods for Codeine Determination

Enhancing the biodegradation of ibuprofen through factor analysis and kinetic modeling

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