Determination of Cobalt in Urine by Flameless Atomic Absorption Spectroscopy. Comparison of Direct Analysis Using Zeeman Background Correction and Indirect Analysis Using Extraction in Organic Solution

This study aimed at investigating the status of non-enzymatic antioxidant agents during experimental caprine coccidiosis. A total of 20 newborn kids were selected and allocated into 2 (diseased and healthy) groups. Ten of the kids were infected with sporulated oocysts of the most pathogenic species of Eimeria and ten served as controls. Blood samples were taken at 0 (before inoculation), 3, 7, 14, 21, 28 and 35 days post infection (dpi) and the concentrations of antioxidant trace elements, antioxidant vitamins and ceruloplasmin were measured. Our data showed remarkable reductions in serum concentrations of some antioxidant trace elements (zinc, manganese and selenium) and vitamins (vitamin C) in the diseased kids, however, significant increases were observed in the serum level of ceruloplasmin in infected animals. These alterations became more prominent at 14 to 21 dpi. These observations suggest that Eimeria parasites can significantly interfere with the levels of some antioxidant trace elements and vitamins during caprine coccidiosis. These changes indicate the overproduction of oxidative radicals during the pathogenesis of Eimeria species that may account for extensive oxidative damage in infected animals.

show abstract

“…One thousand micrograms per milliliter standard solutions of each mineral were used in the measurements. 14,15…”

Section: Trace Elements Measurementmentioning

confidence: 99%

Experimental caprine coccidiosis: the pattern of changes in antioxidant micronutrients and vitamins

2014

View full text Add to dashboard Cite

show abstract

“…Argon was used as the purging gas, and the background absorption was automatically corrected by the Zeeman effect. Standard solutions (1,000 μg/ml) of zinc, copper, cobalt, manganese, and selenium were used in the measurements (Aihara et al 1984;Bouman et al 1986). …”

Section: Trace Element Measurementsmentioning

confidence: 99%

Evaluation of erythrocyte antioxidant mechanisms: antioxidant enzymes, lipid peroxidation, and serum trace elements associated with progressive anemia in ovine malignant theileriosis

et al. 2011

View full text Add to dashboard Cite

Ovine malignant theileriosis is a fatal disease that is characterized by severe progressive anemia. In order to elucidate the underlying mechanisms involved in anemia, this study was designed to assess the antioxidant status and erythrocyte oxidative injuries in Iranian fat-tailed sheep that suffered from malignant theileriosis. The infected animals (infected group), composed of 50 Iranian sheep about 1-2 years old, naturally infected with Theileria sp., were divided into three subgroups according to parasitemia rates (<1%, 1-3%, 3-5%), and ten non-infected animals were also selected as the control group. Blood samples were taken and hematological parameters, the activities of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase, erythrocyte osmotic fragility, and serum concentrations of some trace elements (copper, iron, zinc, manganese, and selenium), were measured. As an index of lipid peroxidation, the level of malondialdehyde (MDA) was also determined. According to the results, a significant decrease in red blood cell (RBC) count, packed cell volume, the activities of SOD, GPX, and catalase (P <0.001), and also serum concentrations of Cu, Zn, Mn, and Se (P < 0.05) were evident in the infected sheep. In contrast, significantly increased levels of MDA and erythrocyte osmotic fragility (P < 0.001) as well as serum concentration of iron (P < 0.05) were recorded in the infected animals. The significant decrease in antioxidant enzyme activities and substantial elevated levels of lipid peroxidation and erythrocyte osmotic fragility associated with the increase in parasitemia indicate increased exposure of RBCs to oxidative damage. Also, it appears that disturbed antioxidant defense mechanisms can promote the development of anemia in ovine theileriosis.

show abstract

“…To ascertain the levels of trace minerals in the serum, serum samples were subjected to digestion using a mixture of perchloric and nitric acid (in a 3:7 ratio). Copper, selenium, and zinc concentrations were quantified utilizing an atomic absorption spectrophotometer (Shimadzu Asc-6100, Japan) (Bouman et al 1986). The results for serum trace mineral levels were reported in µmol/L of serum.…”

Section: Biochemical Parametersmentioning

confidence: 99%

Dynamics of Hematobiochemical and Clinical Patterns in Ewes in Different Reproductive Stages Under Controlled Nvironmental Conditions

2024

Agrobiol. Rec.

View full text Add to dashboard Cite

The study aimed to investigate the clinical patterns and blood profile dynamics in ewes across different reproductive stages. The research involved analyzing clinical patterns, hematological parameters, and various serum parameters from nonpregnancy to postpartum ewes, as well as mid-pregnancy ewes categorized by age (young vs. old) and litter size (single vs. twin). Analysis of serum minerals demonstrated notable changes in the levels of calcium, phosphorus, magnesium, copper, zinc, and selenium across the reproductive cycle. Calcium decreased significantly during late pregnancy (7.11±0.39mg/dL) and postpartum (9.69±0.06mg/dL), phosphorus remained consistent except for a decrease in late pregnancy (4.15±0.36mg/dL), and magnesium declined gradually from nonpregnancy to postpartum. Similarly, the examination of serum leptin, vitamin D, glucose, and triglycerides provided valuable insights into metabolic status and energy homeostasis. Leptin showed a significant increase during pregnancy (5.40±0.31, 5.80±0.06, 6.33±0.09ng/dL). The evaluation of hematological parameters revealed dynamic variations in RBC, Hb, HCT, WBC, lymphocyte, neutrophil, and MID levels throughout the reproductive stages. Additionally, the analysis of clinical patterns including body temperature, pulse rate, respiratory rate, and ruminal movement showed the adaptive changes occurring in response to pregnancy. Furthermore, comparisons between young vs. old age groups and single vs. twin lamb pregnancies indicated limited effects of age and litter size on the measured parameters. In conclusion, this research provides comprehensive insights that contribute to a better understanding of ewe health, reproductive performance, and metabolic adaptations, offering valuable information for effective management strategies, mineral supplementation, and overall care during different reproductive stages.

show abstract

Determination of Cobalt in Urine by Flameless Atomic Absorption Spectroscopy. Comparison of Direct Analysis Using Zeeman Background Correction and Indirect Analysis Using Extraction in Organic Solution

Cited by 14 publications

References 6 publications

Experimental caprine coccidiosis: the pattern of changes in antioxidant micronutrients and vitamins

Experimental caprine coccidiosis: the pattern of changes in antioxidant micronutrients and vitamins

Evaluation of erythrocyte antioxidant mechanisms: antioxidant enzymes, lipid peroxidation, and serum trace elements associated with progressive anemia in ovine malignant theileriosis

Dynamics of Hematobiochemical and Clinical Patterns in Ewes in Different Reproductive Stages Under Controlled Nvironmental Conditions

Contact Info

Product

Resources

About