Determination of cimetidine in human plasma by free capillary zone electrophoresis

Lukša, J.; Josić, Dj.

doi:10.1016/0378-4347(95)00032-e

Cited by 29 publications

(8 citation statements)

References 8 publications

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“…High-performance li-quid chromatography (HPLC) is among those frequently used for the purpose [1,3,4]. The concentration detectability of CE is, however, much poorer than that of HPLC and preconcentration of the analyte by solidphase extraction (SPE) [6] or electrochromatographic SPE [5] was required before CE analysis. Compared with HPLC, CE has advantages such as high separation efficiency, fast analysis speed, less sample consumption, and low operating costs.…”

Section: Introductionmentioning

confidence: 99%

“…Standard solutions of cimetidine (1.0 pg mL -1) in 25 naN buffers of different pH (acetate for pH 3-5, phosphate for pH[5][6][7][8] were applied to the microcolumn for 60s at 1.7mLmin -1. Standard solutions of cimetidine (1.0 pg mL -1) in 25 naN buffers of different pH (acetate for pH 3-5, phosphate for pH[5][6][7][8] were applied to the microcolumn for 60s at 1.7mLmin -1.…”

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confidence: 99%

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Determination of cimetidine in human plasma by use of coupled-flow injection, solid-phase extraction, and capillary zone electrophoresis

Luo

Chen

2001

Chromatographia

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An on-line flow iniection -solid-phase extraction-capillary zone electrophoresis (FI-SPE-CZE) method has been developed for determination of cimetictine in human plasma. Sodium docte-@sulfate (SDS) was used as dynamic chemical modifier for elimination of capillary contamination by biological macromolecules. FI on-line preconcentration and cleaning of the analyte by means of a Cm microcolumn was performed automatically and CZE separation was performed consecutively without interruption of the applied voltage and belween-run-washing of the capillary. A detection limit of 8 l.tg L -1 (3 x ~) was achieved at a sample throughput of 12 h -1. The approach was successfully used for a pharmacokinetic study of cimetictine.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Determination of cimetidine in human plasma by use of coupled-flow injection, solid-phase extraction, and capillary zone electrophoresis

Luo

Chen

2001

Chromatographia

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“…Two studies on the separation of CIM and related compounds (e.g. CSO) by CE have been published [26,27]. Both studies applied a low-pH phosphate buffer; however, the separation of CSO enantiomers was not reported.…”

Section: Chiral Ce Condition (Off-line Method)mentioning

confidence: 99%

Enantioselective in‐line and off‐line CE methods for the kinetic study on cimetidine and its chiral metabolites with reference to flavin‐containing monooxygenase genetic isoforms

et al. 2009

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An in-line screening and an off-line chiral CE method were developed to determine the stereoselectivity of flavin-containing monooxygenase (FMO) isoforms using cimetidine (CIM) as a substrate. The S-oxygenation of CIM was investigated using achiral chemical oxidants and (human supersomes) enzymatic metabolism procedures. In the off-line setup, the chiral selector sulfobutylether-beta-CD was chosen to separate the CIM S-oxide (CSO) metabolites. The electrophoretic migration order of CSO was confirmed to be (+) before (-) through the use of single enantiomers obtained by preparative chromatography. For the electrophoretically mediated microanalysis method, the in-line enzymatic reaction was performed in 100 mM phosphate reaction buffer (pH 8.3), whereas 50 mM phosphate buffer with 30 mM chiral selector (pH 2.5) was used as a BGE. During the screening of FMO isoenzymes by the electrophoretically mediated microanalysis method, formation of the new chiral center on the CIM sulfur was found to be stereoselective. FMO1 produces more (-)-CSO-enantiomer, while FMO3 generates mainly (+)-CSO-enantiomer. On the other hand, FMO5 shows no activity. The kinetic constants of FMO1 and FMO3 were measured by the off-line method. A K(m)=4.31 mM for the formation of the (+)-CSO-enantiomer and a K(m)=4.56 mM for the (-)-CSO-enantiomer are reported for the first time for FMO1.

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“…Several HPLC methods are described for the biological analysis of CIM [8][9][10][11][12][13][14], RAN [10,[15][16][17], FAM [18][19]. Few CE methods for the analysis of CIM [20][21][22][23] and FAM [24] are published. Not to mention on-line concentration, even no available CE method can be used for the simultaneous determination of H 2 -antagonists.…”

Section: Introductionmentioning

confidence: 99%

Head-column field-amplified sample stacking in capillary electrophoresis for the determination of cimetidine, famotidine, nizatidine, and ranitidine-HCl in plasma

et al. 2001

Electrophoresis

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In this study, low concentrations of histamine2-receptor (H2-)antagonists were effected across a water plug, with separation taking place in a binary buffer comprising ethylene glycol and NaH2PO4 (pH 5.0), and detection at 214 nm. Liquid-liquid extraction with ethyl acetate- isopropanol is shown to provide extracts that are sufficiently clean. The calibration curves were linear over a concentration range of 0.1-2.00 microg/mL cimetidine, 0.2-5.0 microg/mL ranitidine-HCl, 0.3-5.0 microg/mL nizatidine, and 0.1-3.0 microg/mL famotidine. Mean recoveries were > 82%, while the intra- and interday relative standard deviations (RSDs) and relative errors (REs) were all < 13%. The method is sensitive with a detection limit of 3 ng/mL cimetidine, 30 ng/mL ranitidine HCl, 50 ng/mL nizatidine and 10 ng/mL famotidine (S/N = 3, electric-driven injection 90 s). This newly developed capillary electrophoresis (CE) method was applied for the determination of analytes extracted from plasma taken from a volunteer dosing a cimetidine, ranitidine, and nizatidine tablet simultaneously. These three H2-antagonists can be detected in real samples by this method, excluding the low dosing of famotidine tablet.

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Determination of cimetidine in human plasma by free capillary zone electrophoresis

Cited by 29 publications

References 8 publications

Determination of cimetidine in human plasma by use of coupled-flow injection, solid-phase extraction, and capillary zone electrophoresis

Determination of cimetidine in human plasma by use of coupled-flow injection, solid-phase extraction, and capillary zone electrophoresis

Enantioselective in‐line and off‐line CE methods for the kinetic study on cimetidine and its chiral metabolites with reference to flavin‐containing monooxygenase genetic isoforms

Head-column field-amplified sample stacking in capillary electrophoresis for the determination of cimetidine, famotidine, nizatidine, and ranitidine-HCl in plasma

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