Determination of chlorogenic acid in rat blood by microdialysis coupled with microbore liquid chromatography and its application to pharmacokinetic studies

Tsai, Tung Hu; Chen, Y.F; Shum, Andrew Yau‐Chik; Chen, C.F

doi:10.1016/s0021-9673(99)01153-x

Cited by 29 publications

(21 citation statements)

References 19 publications

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“…The value of bile recovery test depended on the sampling efficiency of microdialysis system especially dialysis membrane. As reported in some studies, 14,15,[18][19][20] the variability of R dial was stable (S.D 0.9%) and then the sampling technique was suitable for the application of pharmacokinetics. The stability study showed that QC samples were allowed to stand at room temperature for at least 8 h and Ϫ20°C for at least 2 weeks, and stable after at least three freeze-thaw cycles with the overall bias (%) between the observed concentration and nominal value lower than 7.6%.…”

Section: Fig 1 Chemical Structures Of Sweroside (A) and Internal Stmentioning

confidence: 60%

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Determination of Sweroside in Rat Plasma and Bile for Oral Bioavailability and Hepatobiliary Excretion

Luo

Cao

Wen

et al. 2009

CHEMICAL & PHARMACEUTICAL BULLETIN

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79 Notes Sweroside (C 16 H 22 O 9 , molecular weight 358.344), an iridoid glycoside, belongs to a large group of natural monoterpenoids with a glucose moiety attached to C-1 in the pyrane ring. It widely exists in plants of many families [1][2][3] and shows a wide variety of biological activities such as hepatoprotective effect, antitumor, antibacterial, antifungal. 4-7)Sweroside is also the main ingredient of purified herbal extracts from the flower buds of Lonicera japonica THUNB. (IGEs-1) which were demonstrated to possess perfect hepatoprotective effect in our preliminary experiments. Since sweroside and its correlative plant extracts have exhibited these pharmacological effects, it would be hopeful that sweroside and IGEs-1 to be developed to some promising preparations of herbal medicinal products (HMPs).Recently, HMPs are getting more common in the main stream in previously skeptical markets such as the United States and United Kingdom. 8) Establishing the pharmacological basis for efficacy of HMPs is a constant challenge. Of particular interest is the question of bioavailability to assess to what degree and how fast compounds are absorbed after administration of HMPs. Of further interest are the elucidation of metabolic pathways, and the assessment of elimination routes and their kinetics. These data become an important issue to link data from pharmacological assays and clinical effects. A better understanding of the pharmacokinetics and bioavailability of HMPs can also help in designing rational dosage regimens. Despite a wide range of research in either sweroside or L. japonica, 9,10) to our best knowledge, there is no information available on the pharmacokinetics of sweroside orally and intravenously administered to investigate its oral bioavailability and hepatobiliary excretion. Moreover, no report has been published to elucidate the metabolites and the metabolic pathways of sweroside.Furthermore, the development of sampling technique has been a focus in vivo experiment.11) Microdialysis system is a sampling technique that can reduce endogenous interference and can be easy to couple with detector for on-line analysis. A series of papers reported the successful application of microdialysis system to pharmacokinetic study.12-15) However, there is no paper to present the application of microdialysis for the preparation of bile samples of sweroside in rat.In the present study, a simple, stable and accurate HPLC-UV method was developed to determine sweroside in rat plasma, urine, feces and bile simultaneously. The method was applied to basic pharmacokinetic and oral bioavailability studies after intravenous administration of sweroside and oral administration of sweroside and IGEs-1. A sampling method through a validated bile microdialysis system coupled with this HPLC-UV determination was developed to evaluate the hepatobiliary excretion of sweroside in rat. ExperimentalPreparation of IGEs-1 and Sweroside The flower buds of Lonicera japonica THUNB. were collected at the middle of May from Mi county, Hena...

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Section: Fig 1 Chemical Structures Of Sweroside (A) and Internal Stmentioning

confidence: 60%

“…In vivo recovery from bile (R dial ) was calculated by the following equation, R dial ϭ1Ϫ(C out /C in ). 14,18) The stability of the sample was assessed by determining the QC samples under ambient, frozen and freeze-thaw storage.…”

Section: Administration and Preparation Of Samplesmentioning

confidence: 99%

Determination of Sweroside in Rat Plasma and Bile for Oral Bioavailability and Hepatobiliary Excretion

Luo

Cao

Wen

et al. 2009

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…The probe incorporates a short length of dialysis tubing and substances with molecular weight below the cut-off of the dialysis tubing will diffuse through the probe because of the concentration gradient. The microdialysis probe membrane to exclude macromolecules for sampling of unbound drugs and/or their metabolites was developed in our previous work (Tsai et al, 1998(Tsai et al, , 2000Huang et al, 1999). An automatic real-time sampling system was found to be beneficial in minimizing the degradation of cephaloridine.…”

Section: Introductionmentioning

confidence: 99%

Measurement and pharmacokinetic analysis of unbound cephaloridine in rat blood by on‐line microdialysis and microbore liquid chromatography

Tsai¹,

Kao

Chen

2001

Biomedical Chromatography

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A technique involving rapid sampling of cephaloridine in rat blood was achieved using a combination of microdialysis and sensitive microbore liquid chromatography. A microdialysis probe was inserted into the jugular vein/right atrium of a Sprague-Dawley rat. Then after a real-time collection of the analyte by microdialysis, the dialysate was automatically injected into a liquid chromatographic system via an on-line injector. Following a 2 h stabilization period after the surgical procedure, cephaloridine (20 mg/kg, i.v.) was then administered via the femoral vein. Isocratic elution of cephaloridine was carried out with a mobile phase containing methanol-20 mM monosodium phosphate (25:75, v/v, pH 5.5), and the flow rate of the mobile phase was 0.05 mL/min within 10 min. Intra- and inter-assay accuracy and precision of the assay were each less than 10%. The in vivo recovery of the cephaloridine from the microdialysate was 49.7 +/- 8.0% and 42.4 +/- 8.4% for 0.5 and 1 microg/mL standards (n = 6), respectively. Based on the pharmacokinetic analysis, the elimination half-life was 32.2 +/- 8.6 min by cephaloridine administration (20 mg/kg, i.v., n = 6).

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“…The thin-layer chromatography (TLC), [14][15][16][17] high performance liquid chromatography (HPLC), [18][19][20][21][22][23][24][25][26][27] and capillary electrophoresis (CE) [28][29][30][31] have been used to determine CGA, CFA, FRA, PCA and PAD in their intact materials or Chinese herbal preparations. To the best of our knowledge, there were no reports on simultaneous determination of the five marker compounds in Chinese herbal preparations or FFPM by HPLC.…”

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confidence: 99%

Simultaneous Determination of Chlorogenic Acid, Caffeic Acid, Ferulic Acid, Protocatechuic Acid and Protocatechuic Aldehyde in Chinese Herbal Preparation by RP-HPLC

Yu²,

Jin-yin³

et al. 2004

CHEMICAL & PHARMACEUTICAL BULLETIN

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Determination of chlorogenic acid in rat blood by microdialysis coupled with microbore liquid chromatography and its application to pharmacokinetic studies

Cited by 29 publications

References 19 publications

Determination of Sweroside in Rat Plasma and Bile for Oral Bioavailability and Hepatobiliary Excretion

Determination of Sweroside in Rat Plasma and Bile for Oral Bioavailability and Hepatobiliary Excretion

Measurement and pharmacokinetic analysis of unbound cephaloridine in rat blood by on‐line microdialysis and microbore liquid chromatography

Simultaneous Determination of Chlorogenic Acid, Caffeic Acid, Ferulic Acid, Protocatechuic Acid and Protocatechuic Aldehyde in Chinese Herbal Preparation by RP-HPLC

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