Simultaneous Determination of Chlorogenic Acid, Caffeic Acid, Ferulic Acid, Protocatechuic Acid and Protocatechuic Aldehyde in Chinese Herbal Preparation by RP-HPLC

Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH· reduction

“…B7 and B8: aqueous extract of tea dust at 330 nm and 275 nm respectively. method for the simultaneous determination of chlorogenic acid, caffeic acid and caffeine proved highly sensitive, the limits of detection and quantitation being lower than in similar methods published elsewhere (Pellati et al, 2005, Li et al, 2004, Potard et al, 1999 and the retention time was about 5 minutes.…”

“…When the first mobile phase, methanol:0.2 mol/L acetate buffer, pH 3.6 (15:85 v/v), was used and column output monitored at λ = 300 nm (Li et al, 2004), some material was retained on the column and the retention time exceeded 70 min. With methanol:water:acetic acid (19:81:1.5 v/v) and λ = 240 nm (Li et al, 2005), the retention time exceeded 20 min and the resolution was poor.…”

“…The leaf extract of I. paraguariensis was shown to be absorbed and to be active in vivo when healthy patients drank it, and one hour later blood was collected and the LDL content analysed in relation to lipid peroxidation (Gugliucci, 1996). The Ilex paraguariensis aqueous extract inhibited the lipid peroxidation induced by the Fe 2+ / ascorbate system and that induced by the CCl 4 /NADPH system, in both cases detected by the thiobarbituric acid assay (Schinella et al, 2000) For the simultaneous determination of CGA (chlorogenic acid) and CFA (caffeic acid) (Figure 1) by HPLC, various sets of chromatographic conditions have been used in recent work: in Li et al (2004), the mobile phase was a mixture of methanol and 0.2 mol/L acetate buffer (pH 3.6) (15:85, v/v) flowing at 1.0 mL/min, the detection wavelength (λ) being set at 300 nm; in Li et al (2005), methanol:water:acetic acid (19:81:1.5, v/v) was used, flowing at 1.0 mL/min, with λ at 240 nm, and in Pellati et al (2005), aqueous phosphoric acid solution (0.1%) and acetonitrile (90:10 v/v), flowing at 1.5 mL/min, with the detector at 330 nm. Another method, for the simultaneous determination of CGA, CFA and CAF (caffeine), used a linear gradient, the mobile phase changing from 100% solvent A (water:acetic acid, 95:5) to 100% solvent B (methanol:acetic acid, 95:5) over a period of 65 min at a flow rate of 1 mL/min (Variyar et al, 2003).…”

A stability-indicating HPLC-PDA method for the determination of ferulic acid in chitosan-coated poly(lactide-co-glycolide) nanoparticles

“…FA has been quantified by many analytical methods, such as high-performance liquid chromatography (HPLC) coupled with photodiode array wavelength detector (PDA) or UV-Vis detector (Anselmi et al, 2006;Craparo et al, 2009;Kareparamban et al, 2013;Li et al, 2007;Li et al, 2004;Li, Bi, 2003;Lu et al, 2005;Nadal et al, 2015;Picone et al, 2009;Qi et al, 2007;Wang et al, 2011), liquid chromatography tandem mass spectrometry (Guy et al, 2009;Wang et al, 2013;Zhang et al, 2009), UV-Vis spectroscopy (Lima et al, 2013;Merlin et al, 2012), thin layer chromatography (Mabinya, Mafunga, Brand, 2006), high-performance thin layer chromatography (Hingse, Digole, Annapure, 2014;Srivastava, Singh, Singh Rawat, 2012), gas chromatography (Olthof et al, 2003), chemiluminescence (Shen et al, 2013), capillary electrophoresis (Lima, Duarte, Esteves, 2007), micellar electrokinetic chromatography (Guo et al, 2003), electrochemical analysis (Vilian, Chen, 2014) and paper-based platforms (Tee-Ngam et al, 2013). However, HPLC is considered the most reliable and popular methodology for investigating phenolic acids (Barberousse et al, 2008).…”

Simultaneous Determination of Components in Preparation Naodesheng Injection by High Performance Liquid Chromatograph–Atmospheric Pressure Chemical Ionization Mass Spectrometry (HPLC-MS/APCI)

“…[1][2][3][4][5] So far, it is widely accepted that multiple constituents are responsible for the therapeutic effects of TCM, and to ensure its quality, therefore, it is necessary to quantitatively determine the multiple bioactive components of TCM. [6][7][8] Naodesheng injection is a composite formula of TCM preparation comprising five craw materials or extracts including Radix Puerariae Lobatae, Flos Carthami, Radix et Rhizoma Notoginseng, Rhizoma Chuanxiong, and Fructus Crataegi, which is efficient in the treatment of cerebral arteriosclerosis, ischemic cerebral stroke, and apoplexy linger effect.…”