Determination of Chlorinated Phenols in Surface-Treated Lumber by HPLC

Daniels, C. R.; Swan, E. P.

doi:10.1093/chromsci/17.11.628

Cited by 13 publications

(1 citation statement)

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“…Growth was determined as increase in turbidity at 600 nm (OD600) using a Hitachi UH5300 spectrophotometer and/or the detection of reduced product(s) transformed from a potential electron acceptor by comparing to uninoculated abiotic incubations. Chlorinated compounds were analysed using an Agilent 7890B GC-FID or an Agilent 1260 II HPLC system [42,43]. Reduction of elemental sulphur or an oxidized sulphur compound was analysed by measuring the formation of sulphide using the modified methylene blue method as described [44].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil

Jin

Huo

Yang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Two anaerobic, tetrachloroethene- (PCE-) respiring bacterial isolates, designated strain ACSDCE T and strain ACSTCE, were characterized using a polyphasic approach. Cells were Gram-stain-negative, motile, non-spore-forming and shared a vibrioid- to spirillum-shaped morphology. Optimum growth occurred at 30 °C and 0.1–0.4 % salinity. The pH range for growth was pH 5.5–7.5, with an optimum at pH 7.2. Hydrogen, formate, pyruvate and lactate as electron donors supported respiratory reductive dechlorination of PCE to cis-1,2-dichloroethene (cDCE) in strain ACSDCE T and of PCE to trichloroethene (TCE) in strain ACSTCE. Both strains were able to grow with pyruvate under microaerobic conditions. Nitrate, elemental sulphur, and thiosulphate were alternative electron acceptors. Autotrophic growth was not observed and acetate served as carbon source for both strains. The major cellular fatty acids were C16 : 1 ω7c, C16 : 0, C14 : 0 and C18 : 1 ω7c. Both genomes feature a circular plasmid. Strains ACSDCE T and ACSTCE were previously assigned to the candidate species 'Sulfurospirillum acididehalogenans'. Here, based on key genomic features and pairwise comparisons of whole-genome sequences, including average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity, strains ACSDCE T and ACSTCE, 'Ca. Sulfurospirillum diekertiae' strains SL2-1 and SL2-2, and the unclassified Sulfurospirillum sp. strain SPD-1 are grouped into one distinct species separate from previously described Sulfurospirillum species. Compared to Sulfurospirillum multivorans and Sulfurospirillum halorespirans , which dechlorinate PCE to cDCE without substantial TCE accumulation, these five strains produce TCE or cDCE as the end product. In addition, some cellular fatty acids (e.g., C16 : 0 3OH, C17 : 0 iso 3OH, C17 : 0 2OH) were detected in strains ACSDCE T and ACSTCE but not in other Sulfurospirillum species. On the basis of phylogenetic, physiological and phenotypic characteristics, 'Ca. Sulfurospirillum acididehalogenans' and 'Ca. Sulfurospirillum diekertiae' are proposed to be merged into one novel species within the genus Sulfurospirillum , for which the name Sulfurospirillum diekertiae sp. nov. is proposed. The type strain is ACSDCE T (=JCM 33349T= KCTC 15819T=CGMCC 1.5292T).

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Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil

Jin

Huo

Yang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Determination of total pentachlorophenol in the urine of workers

Drummond

Roosmalen

Kornicki

1982

Int. Arch Occup Environ Heath

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Free pentachlorophenol (PCP) represents a small and variable fraction of total PCP excreted in the urine of exposed workers. A method incorporating hydrolysis is essential to be able to relate PCP excretion to absorbed dose. 3,5-Dichloro-2,4,6-tribromophenol (DTP) is added as an internal standard to a urine sample which is then acidified and steam-distilled. The distillate is made alkaline and extracted with methylene chloride to remove interferences. The distillate is then acidified and the phenols extracted into methylene chloride. The extract is evaporated, redissolved in acetonitrile and the PCP is measured using high pressure liquid chromatography (HPLC) using a 15 X 0.46 cm Spherisob-ODS 5 microns column with isocratic elution (H2O:CH3CN:CH3CO2H, 45:55:0.3 v/v). Detection is by fixed wavelength detector at 313 nm and calculation by the method of internal standardization. The biological threshold value used to trigger action to reduce exposure is 7 mg/l (corrected to a specific gravity of 1.024).

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A DTA study of phenols

Buckman¹,

Hill

Magee

1990

Journal of Thermal Analysis

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A comprehensive DTA study is reported of 17 halophenols and of the corresponding pnitrobenzoylchloride 3,5-dinitrobenzoylehloride and p-phenylazobenzoylchloride derivatives, prepared ~in-situ'by heating intimate mixtures of phenol and acid chloride in the DTA system. The thermal analysis data, in particular, the derivative formation temperatures, are interpreted in terms of the inductive, mesomeric and sterie effects associated with the ring substituents of the phenol and acid chloride and the extent of inter-and intramolecular hydrogen-bonding existing in these systems. The DTA data collectively provide a reference base for the identification and characterization of halophenols via thermal analysis.

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Determination of Chlorinated Phenols in Surface-Treated Lumber by HPLC

Cited by 13 publications

References 0 publications

Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil

Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil

Determination of total pentachlorophenol in the urine of workers

A DTA study of phenols

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