Determination of chloramphenicol in honey by liquid chromatography–tandem mass spectrometry

Forti, Anna Francesca; Campana, Guido; Simonella, A.; M, Multari; Scortichini, Giampiero

doi:10.1016/j.aca.2004.10.059

Cited by 89 publications

(44 citation statements)

References 11 publications

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“…Several methods have been used to detect residue of chloramphenicol in honey and other matrices (7,12,13,14). However, an enzyme immunoassay is now considered to be the most sensitive and has been used for the last few decades.…”

Section: Discussionmentioning

confidence: 99%

ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Biernacki

2015

Bulletin of the Veterinary Institute in Pulawy

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An analytical validation of a screening ELISA for detection of chloramphenicol (CAP) in honey was conducted according to the Commission Decision 2002/657/EC and Guidelines for the Validation of Screening Methods for Residues of Veterinary Medicines. The analyte was extracted from honey with a water and ethyl acetate mixture, and CAP concentrations were measured photometrically at 450 nm. The recovery rate of the analyte from spiked samples was 79%. The cut-off level of CAP in honey as the minimum recovery (0.17 units) was established. Detection capability (CCβ) was fixed at 0.25 µg kg -1 . No relevant interferences between matrix effects and structurally related substances including florfenicol and thiamphenicol were observed. The ELISA method should be useful for determination of CAP residues in honey monitoring.

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Section: Discussionmentioning

confidence: 99%

ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Biernacki

2015

Bulletin of the Veterinary Institute in Pulawy

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“…Some methods describe direct extraction and clean-up of chloramphenicol from honey with C 18 SPE (Bogusz et al, 2004) or use of C 18 SPE for further clean-up of an ethyl acetate extract (Ortelli et al, 2004) or of a dichloromethane/acetone extract (Forti et al, 2005) of the honey samples. Other methods involve use of supported liquid-liquid extraction (LLE) on diatomaceous earth cartridges (Kaufmann and Butcher, 2005;Vivekanandan et al, 2005), MIP (Shi et al, 2010) and IAC (Mackie et al, 2013) techniques.…”

Section: Confirmatory Methodsmentioning

confidence: 99%

Scientific Opinion on Chloramphenicol in food and feed

2014

EFS2

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Chloramphenicol is an antibiotic not authorised for use in food-producing animals in the European Union (EU). However, being produced by soil bacteria, it may occur in plants. The European Commission asked EFSA for a scientific opinion on the risks to human and animal health related to the presence of chloramphenicol in food and feed and whether a reference point for action (RPA) of 0.3 µg/kg is adequate to protect public and animal health. Data on occurrence of chloramphenicol in food extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF) were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which chloramphenicol is present at 0.3 µg/kg in all foods of animal origin, foods containing enzyme preparations and foods which may be contaminated naturally. The mean chronic dietary exposure for this worst-case scenario would range from 11 to 17 and 2.2 to 4.0 ng/kg b.w. per day for toddlers and adults, respectively. The potential dietary exposure of livestock to chloramphenicol was estimated to be below 1 µg/kg b.w. per day. Chloramphenicol is implicated in the generation of aplastic anaemia in humans and causes reproductive/hepatotoxic effects in animals. Margins of exposure for these effects were calculated at 2.4 × 10 5 or greater and the CONTAM Panel concluded that it is unlikely that exposure to food contaminated with chloramphenicol at or below 0.3 µg/kg is a health concern for aplastic anaemia or reproductive/hepatotoxic effects. Chloramphenicol exhibits genotoxicity but, owing to the lack of data, the risk of carcinogenicity cannot be assessed. The SUMMARYChloramphenicol is a broad-spectrum antibiotic effective against Gram-positive and Gram-negative bacteria and, in the past, has been widely used to treat infections in both humans and animals. Chloramphenicol is not authorised for use in food-producing animals in the European Union (EU) but may be used in human medicine and in treatments for non-food-producing animals. Apart from its potential occurrence as a residue in food from illicit treatment of food-producing animals, chloramphenicol has also been used in feed and food enzyme products and may occur naturally in plants from its production by the soil bacterium Streptomyces venezuelae.The EFSA Scientific Opinion entitled "Guidance on methodological principles and scientific methods to be taken into account when establishing Reference Points for Action (RPAs) for non-allowed pharmacologically active substances present in food of animal origin" identified an approach for establishing RPAs for various categories of non-allowed pharmacologically active substances. However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances causing blood dyscrasias (aplastic anaemia) such as chloramphenicol. As chloramphenicol is excluded fro...

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“…Mais recentemente tem sido empregado o método de varredura e confirmação por ressonância de superfície por plasma ("surface plasmon resonance"), que utiliza biossensores específi-cos, providos com uma camada de cloranfenicol derivativo e um anticorpo que possui alta reatividade com o metabólito cloranfenicol glucoronídeo. Este último método, tem sido comparado com CLAE-EM/EM ou empregado em conjunto 32,33 . A técnica de CLAE-EM/EM vem se destacando devido à sua alta especificidade analítica quando utilizada em modo "Multiple Reaction Monitoring" (MRM), no qual os analisadores de massas Q1 e Q3, selecionam os íons precursor e produto, respectivamente, definindo uma transição de m/z específica.…”

Section: O Composto Cloranfenicol (Cap) D-(-)-treo-22-dicloro-n-unclassified

“…A proposta deste trabalho é a divulgação da técnica de CLAE-EM/EM como uma excelente alternativa para análise de baixos níveis de CAP em matrizes de alimentos, como também, a aplicação desta tecnologia para avaliação de algumas amostras comerciais de leite e mel do mercado nacional, visto que as principais figuras de mérito desta técnica são alta detectabilidade, seletividade e geração de resultados auto-confirmatórios. O método emprega extração em fase sólida (EFS) em estireno-divinilbenzeno para pré-concentração e purificação das amostras de leite 16,19,25,31,[33][34][35][36] e extração líquido-líquido, com utilização mínima de solvente orgâni-co, para amostras de mel 28,29,32,33 .…”

Section: O Composto Cloranfenicol (Cap) D-(-)-treo-22-dicloro-n-unclassified

Determinação de resíduos de cloranfenicol em amostras de leite e mel industrializados utilizando a técnica de espectrometria de massas em "tandem" (CLAE-EM/EM)

et al. 2006

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Determination of chloramphenicol in honey by liquid chromatography–tandem mass spectrometry

Cited by 89 publications

References 11 publications

ELISA validation and determination of cut-off level for chloramphenicol residues in honey

ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Scientific Opinion on Chloramphenicol in food and feed

Determinação de resíduos de cloranfenicol em amostras de leite e mel industrializados utilizando a técnica de espectrometria de massas em "tandem" (CLAE-EM/EM)

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