Determination of celiprolol in human plasma using high performance liquid chromatography with fluorescence detection for clinical application

Abstract

A new method of analysis has been developed and validated for the determination of plasma celiprolol concentration. Plasma samples (1 ml) were pre-purified by solid-phase extraction with Bond Elut C18. The separation was achieved with XBridge C18 column (150 mm × 3.0mm i.d., 3.5 μm) at 35 °C using a mixture of acetonitrile and 10mM ammonium acetate buffer (pH 10.5) (34:66, v/v) under isocratic conditions at a flow rate of 0.4 ml/min. The peak was detected using a fluorescence detector at excitation 250 nm and emission 482 nm. Retention times for the internal standard (acebutolol) and celiprolol were 4.2 min and 6.3 min, respectively. Calibration curves were linear over the range of 1.0-1000 ng/ml (r>0.999), with a limit of quantification at 1.0 ng/ml. Intra- and inter-assay precision (relative standard deviation) were less than 13.3% and the accuracy (relative error) was -5.1% to 11.5% at four different concentrations. This proposed method was successfully applied to a study of pharmacokinetic interactions between celiprolol and apple juice in humans.

Small-Dosing Clinical Study: Pharmacokinetic, Pharmacogenomic (SLCO2B1 and ABCG2), and Interaction (Atorvastatin and Grapefruit Juice) Profiles of 5 Probes for OATP2B1 and BCRP

“…A double-peak phenomenon was observed for celiprolol only, which was consistent with our and other previous findings. 5,22,23 Regional differences have been reported in the intestinal mRNA expression of OATP2B1; Meier et al 24 measured the increased expression of OATP2B1 longitudinally along the intestine (ileum > colon descendens ¼ colon transversum > colon ascendens ¼ duodenum). Although the reason for this currently remains unclear, the site dependence of intestinal absorption is the most likely cause.…”

Determination of domperidone in human plasma using high performance liquid chromatography with fluorescence detection for clinical application

“…We had already a developed HPLC method with alkaline conditions for the analysis of basic compound [15,16] in human plasma, by using XBridge™ C18 (5µm, 150mm x 4.6mm i.d. ; Waters) with fluorescence detection.…”

“…In clinical pharmacokinetic studies that require a large number of sample analyses, the SPE method reported here may be suitable because of its labor-and time-saving advantages. We have already successfully used SPE method with Bond Elut C18 ® column for analysis fexofenadine [15] and celiprolol [16]. In the present study, the Bond Elut C18 ® column was applied for the determination of domperidone in human plasma.…”

Detection and quantitation methods for -blockers

“…Itohda and colleagues [1] conducted an investigation employing Solid-Phase Extraction (SPE) to determine the concentration of celiprolol in human plasma. The quantification of this analyte was accomplished through the utilization of High-Performance Liquid Chromatography (HPLC) in conjunction with a fluorescence detector (FLD).…”