Determination of Cefpodoxime Levels and Cefpodoxime Stability In Human Urine By Direct Injection HPLC with Column-Switching

Bombardt, Paul A.; Cathcart, K. S.; Bothwell, Brian E.; Closson, S. K.

doi:10.1080/01483919108049650

Journal of Liquid Chromatography

1991

DOI: 10.1080/01483919108049650

|View full text |Cite

Determination of Cefpodoxime Levels and Cefpodoxime Stability In Human Urine By Direct Injection HPLC with Column-Switching

Paul A. Bombardt¹,

K. S. Cathcart²,

Brian E. Bothwell³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1994

2014

Publication Types

Select...

Article6

Relationship

Self Cite0

Independent6

Authors

Journals

Cited by 16 publications

(1 citation statement)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concentrations of cefpodoxime in plasma and in urine samples were determined by reversed-phase high-performance liquid chromatography methods (1,14). Standard curves were linear from 0.010 to 5.0 g/ml for the plasma method and from 0.50 to 50 g/ml for the urine assay, with correlation coefficients greater than 0.998.…”

mentioning

confidence: 99%

Effect of food on absorption of cefpodoxime proxetil oral suspension in adults

Borin

Forbes

1995

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The effect of a high-fat meal on absorption of a 200-mg dose of cefpodoxime proxetil oral suspension was evaluated in 20 healthy, male volunteers in a randomized, two-way crossover study. The concentrations of cefpodoxime in plasma and in urine were determined by sensitive and specific high-performance liquid chromatography methods. The area under the plasma drug concentration-time curve, time to peak concentration, and urinary excretion of cefpodoxime were significantly greater (P Յ 0.05) after administration of cefpodoxime proxetil oral suspension with food than under fasting conditions. However, the difference in the areas under the curve between fed and fasted treatments was only 11%, and application of the two one-sided tests procedure showed bioequivalence between treatments for this parameter. The slight increase in the extent of drug absorption and the slower rate of absorption which results when cefpodoxime proxetil is given with food are unlikely to be of clinical importance.

show abstract

mentioning

confidence: 99%

Effect of food on absorption of cefpodoxime proxetil oral suspension in adults

Borin

Forbes

1995

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

The bioavailability of cefpodoxime proxetil tablets relative to an oral solution

Borin

Forbes

Hughes

1995

Biopharm & Drug Disp

View full text Add to dashboard Cite

The bioavailability of cefpodoxime proxetil tablets relative to an oral solution of cefpodoxime proxetil in a sucrose/alcohol/citric acid vehicle was studied in 11 healthy volunteers in a randomized, crossover study. Fasted subjects took one cefpodoxime proxetil 100 mg tablet or 50 mL of a 2 mg mL-1 cefpodoxime proxetil oral solution on two separate occasions. In a third study period, all subjects took a 100 mg dose of the oral solution with a high-fat meal to investigate the effect of food on cefpodoxime proxetil absorption from the oral solution. Serial blood samples were obtained over a 24 h period, and urine was collected for 48 h after dosing. Cefpodoxime concentrations in plasma and in urine were determined using HPLC methods. The bioavailability of cefpodoxime proxetil tablets relative to the oral solution was 82%, as determined from AUC ratios. There was no difference in the rate of cefpodoxime absorption between dosage forms. Food had no effect on the extent of drug absorption from the oral solution but did result in delayed absorption. These results suggest that complete dissolution of cefpodoxime proxetil is critical for optimal bioavailability.

show abstract

Recent bioanalytical methods for quantification of third‐generation cephalosporins using HPLC and LC‐MS(/MS) and their applications in pharmacokinetic studies

Jin

Maeng

2014

Biomedical Chromatography

View full text Add to dashboard Cite

Third-generation cephalosporins are semi-synthetic antibiotics with enhanced activity against Gram-negative organisms. The cephalosporins in biological samples are mostly determined using high-performance liquid chromatography (HPLC) and HPLC-mass spectrometry (LC-MS) or tandem mass spectrometry (LC-MS/MS). In recent years, numerous bioanalytical methods have been developed to improve the sensitivity and specificity of cephalosporin quantification using the powerful LC-MS/MS systems that are common in research laboratories. This review article presents recently developed bioanalytical methods by HPLC or LC-MS(/MS) for 12 third-generation cephalosporins, including five oral third-generation cephalosporins, and further discusses their application in pharmacokinetic studies of animals and humans.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Determination of Cefpodoxime Levels and Cefpodoxime Stability In Human Urine By Direct Injection HPLC with Column-Switching

Cited by 16 publications

References 4 publications

Effect of food on absorption of cefpodoxime proxetil oral suspension in adults

Effect of food on absorption of cefpodoxime proxetil oral suspension in adults

The bioavailability of cefpodoxime proxetil tablets relative to an oral solution

Recent bioanalytical methods for quantification of third‐generation cephalosporins using HPLC and LC‐MS(/MS) and their applications in pharmacokinetic studies

Contact Info

Product

Resources

About