Determination of cefaclor in human plasma by a sensitive and specific liquid chromatographic–tandem mass spectrometric method

Chen, Xiaoyan; Zhong, Dafang; Huang, Bin; Cui, Jian

doi:10.1016/s1570-0232(02)00656-6

Cited by 28 publications

(21 citation statements)

References 7 publications

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“…The cellular content of amoxicillin and cefaclor was quantified using a modified LC/MS method of Chen et al (2003). Briefly, 10 l of each sample was injected onto Restek Ultra Aqueous C 18 column (200 mm ϫ 2.1 mm, 5 ; Restek Corp., PA) connected to an Agilent 1100 LC-MS system (Agilent Technologies, Palo Alto, CA).…”

Section: Materials [mentioning

confidence: 99%

Interactions of Amoxicillin and Cefaclor With Human Renal Organic Anion and Peptide Transporters

Li¹,

Anderson²,

Phillips³

et al. 2006

Drug Metab Dispos

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ABSTRACT:Amoxicillin and cefaclor are two of the widely used ␤-lactam antibiotics in the treatment of urinary tract infections. Both drugs are eliminated mainly by the kidney and rely on renal excretion to exert their antibacterial activities in the urinary tract. Previous studies have suggested the involvement of organic anion and oligopeptide transporters in membrane transport of ␤-lactams. The objective of the current study was to examine the kinetics of amoxicillin and cefaclor interactions with human renal transporters human organic anion transporter 1 (hOAT1), human peptide transporter 1 (hPepT1), and human peptide transporter 2 (hPepT2) in detail, both as substrates and as inhibitors. Using fluorescence protein tagging and cell sorting, we established Madin-Darby canine kidney cell lines stably expressing highly functional hOAT1, hPepT1, and hPepT2. Amoxicillin and cefaclor inhibited hOAT1-mediated [ 3 H]para-aminohippuric acid uptake (K i ‫؍‬ 11.0 and 1.15 mM, respectively). However, our uptake study revealed that neither drug was transported by hOAT1. Amoxicillin and cefaclor competitively inhibited hPepT2-mediated [ 3 H]glycylsarcosine uptake (K i ‫؍‬ 733 and 65 M, respectively), whereas much lower affinity for hPepT1 was observed with both antibiotics. Direct uptake studies demonstrated that amoxicillin and cefaclor were transported by hPepT1 and hPepT2. Kinetic analysis showed that hPepT2-mediated uptake of both drugs was saturable with K m of 1.04 mM for amoxicillin and 70.2 M for cefaclor. hPepT2, and to a lesser extent hPepT1, may play an important role in apical transport of amoxicillin and cefaclor in the renal tubule. hOAT1, in contrast, is not involved in basolateral uptake of these antibiotics.

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Section: Materials [mentioning

confidence: 99%

Interactions of Amoxicillin and Cefaclor With Human Renal Organic Anion and Peptide Transporters

Li¹,

Anderson²,

Phillips³

et al. 2006

Drug Metab Dispos

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“…The previous methods often lack sufficient sensitivity and specificity to measure low cephradine concentrations in plasma. Recent studies have used high-performance liquid chromatography (HPLC) [15]- [22] and liquid chromatography tandem mass spectrometry (LC-MS/MS) [23] [24] in order to increase the sensitivity to detect cephradine in biological materials. However, these methods have disadvantages as follows.…”

Section: Cephradine ((6rmentioning

confidence: 99%

Clinical Pharmacokinetic and Bioequivalence Studies of Two Brands of Cephradine in Healthy Korean Using HPLC Method

Kim¹,

Kim²,

Kim³

et al. 2018

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The goal of our research was to compare the pharmacokinetics and evaluate the bioequivalence of two brands of cephradine 500 mg capsules in 24 normal Korean volunteers. The plasma samples were acquired at 13 time points for 8 h after administration. The concentrations of cephradine in human plasma were measured by a high-performance liquid chromatography (HPLC). Isocratic mobile phase which consisted of acetonitrile, methanol, and 20 mM potassium phosphate (15/5/80, v/v/v, pH 3.48) was used to separate the analytical column cosmosil cholester (250 × 4.6 mm, 3 µm). Analytes were detected in ultraviolet (260 nm). The novel analytical method was described as simple sample preparation, a short retention time (less than 6 min) and making it suitable for use in clinical trials. Pharmacokinetic parameters, such as ) were determined for the reference and test drugs in plasma. Pharmacokinetic parameters with a 90% confidence interval were 87% -95% for AUC 0-t and 91% -115% for C max . They were satisfied within the bioequivalence range 80% -125% of the KFDA guidelines. Therefore, our HPLC method was well applied in a bioequivalence and pharmacokinetic study of two formulations in normal subjects.

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“…1), but differ in the nature of substituent at the 3-and/or 7-positions of the cephem ring (Delgad and Wilson, 2004;Dollery,1999). Several methods have been described for the quantitative determination of cephalosporins included spectrophotometry (Ayad et al,1999;Saleh et al, 2001Saleh et al, , 2003, spectrofluorimetry (Aly et al,1996), high performance liquid chromatography (Baranowska et al, 2006;Chen et al, 2003;De Diego Glari´a et al, 2005;Misztal, 1998;Moore et al,1991;Sørensen and Snor, 2000;Tsai and Chen, 2000), potentiometry, (Lima et al, 1998) and voltammetry (Ö zkan et al, 2000). These methods were time-consuming, tedious, and dedicated to sophisticated and expensive analytical instruments .…”

Section: Introductionmentioning

confidence: 99%

New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations

Ahmed

Elbashir

Aboul‐Enein

2015

Arabian Journal of Chemistry

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A simple, accurate, and precise spectrophotometric method has been proposed for the determination of three cephalosporins, namely: cefixime (cefi), cephalexine, (ceph) and cefotaxime (cefo) in pharmaceutical formulations. Proposed method is based on the derivatization of cephalosporins with 1,2-naphthoquinone-4-sulfonic (NQS). The optimum experimental conditions have been studied. Beer's law is obeyed over the concentration of 0.5-3, 0.8-2.8, and 0.2-1.2 lg/mL for cefi, ceph, and cefo, respectively.The detection limits were 0.12, 0.168, and 0.0465 lg/mL for cefi, ceph, and cefo, respectively, with a linear regression correlation coefficient of 0.9993, 0.9993, and 0.9994 and recovery in range from 96.5-102.3, 96.04-102.22, and 97.09-99.3 for cefi, ceph, and cefo, respectively. Effects of pH, temperature, reaction time, and NQS concentration on the determination of cefi, ceph, and cefo, have been examined. This method is simple and can be applied for the determination of cefi, ceph, and cefo in pharmaceutical formulations in quality control laboratories.

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Determination of cefaclor in human plasma by a sensitive and specific liquid chromatographic–tandem mass spectrometric method

Cited by 28 publications

References 7 publications

Interactions of Amoxicillin and Cefaclor With Human Renal Organic Anion and Peptide Transporters

Interactions of Amoxicillin and Cefaclor With Human Renal Organic Anion and Peptide Transporters

Clinical Pharmacokinetic and Bioequivalence Studies of Two Brands of Cephradine in Healthy Korean Using HPLC Method

New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations

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