New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations

Abstract

A simple, accurate, and precise spectrophotometric method has been proposed for the determination of three cephalosporins, namely: cefixime (cefi), cephalexine, (ceph) and cefotaxime (cefo) in pharmaceutical formulations. Proposed method is based on the derivatization of cephalosporins with 1,2-naphthoquinone-4-sulfonic (NQS). The optimum experimental conditions have been studied. Beer's law is obeyed over the concentration of 0.5-3, 0.8-2.8, and 0.2-1.2 lg/mL for cefi, ceph, and cefo, respectively.The detection limits were 0.12, 0.168, and 0.0465 lg/mL for cefi, ceph, and cefo, respectively, with a linear regression correlation coefficient of 0.9993, 0.9993, and 0.9994 and recovery in range from 96.5-102.3, 96.04-102.22, and 97.09-99.3 for cefi, ceph, and cefo, respectively. Effects of pH, temperature, reaction time, and NQS concentration on the determination of cefi, ceph, and cefo, have been examined. This method is simple and can be applied for the determination of cefi, ceph, and cefo in pharmaceutical formulations in quality control laboratories.

Development and Validation of Spectrophotometric Method for Determination of Oxyfluorfen Herbicide Residues

“…1,2-naphthoquinone-4-sulfonate (NQS) ( Figure 1b) has been used as a color-developing reagent in the spectrophotometric determination of many pharmaceutical amines [13][14][15][16][17][18][19][20][21][22][23][24][25][26]. In depth review regarding the applications of NQS for determination of pharmaceutical bearing amine group has recently been reviewed by Elbashir et al [21].…”

Optimization and validation of spectrofluorimetric method for determination of cefadroxile and cefuroxime sodium in pharmaceutical formulations

“…Several methods have been described for the quantitative determination of cephalosporins, including spectrophotometry , spectrofluorimetry , high‐performance liquid chromatography and voltammetry . These methods are time‐consuming, tedious and require sophisticated and expensive analytical instruments.…”

A New Spectrophotometric Method for Determination of Penicillamine in Pharmaceutical Formulation Using 1, 2-naphthoquine-4-sulfonate (NQS)

“…NQS has been used as a color-developing reagent in the spectrophotometric determination of many pharmaceutical amines [31][32][33][34][35][36][37][38][39]. The applications of NQS for determination of pharmaceutical bearing amine group have recently been reviewed by Elbashir et al [39].…”