Determination of carotenoids and all-trans-retinol in fish eggs by liquid chromatography–electrospray ionization–tandem mass spectrometry

Li, Hongxia; Tyndale, Sélène T.; Heath, Daniel D.; Letcher, Robert J.

doi:10.1016/j.jchromb.2004.11.005

Cited by 63 publications

(37 citation statements)

References 29 publications

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“…For the stationary phase, both normal-phase HPLC and reversed-phase HPLC are used, the elution being either isocratic or gradient; the latter is employed especially for complex extracts containing carotenoids of widely different polarities (Rodríguez-Bernaldo de Quirós and Costa, 2006). Additionally, the carotenoides can be identified by their UV-vis absorption spectra and the presence of trans-astaxanthin can be confirmed by the mass spectrometry detection system (Lacker et al, 1999;Li et al, 2005). With reference to the source from astaxanthin, HPLC studies are mainly in relation to alga and red yeast (Lai et al, 2004), with a few studies on crustacean and fish (Luterotti et al, 1999;ArmentaLópez et al, 2002;Stepnowski et al, 2004;Breithaupt, 2004;Birkeland and Bjerkeng, 2004;Lin et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Quantification of astaxanthin in shrimp waste hydrolysate by HPLC

López-Cervantes

Sánchez-Machado

Gutiérrez-Coronado

et al. 2006

Biomedical Chromatography

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In the present study, a simple and rapid reversed-phase HPLC method for the determination of astaxanthin in shrimp waste hydrolysate has been developed and validated. The analytical procedure involves the direct extraction of astaxanthin from the lipid fraction with methanol. The analytical column, SS Exil ODS, was operated at 25C. The mobile phase consisted of a mixture of water:methanol:dichloromethane:acetonitrile (4.5:28:22:45.5 v/v/v/v) at a flow rate of 1.0 mL/min. Detection and identification were performed using a photodiode array detector (lambda(detection) = 476 nm). The proposed HPLC method showed adequate linearity, repeatability and accuracy.

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Section: Introductionmentioning

confidence: 99%

Quantification of astaxanthin in shrimp waste hydrolysate by HPLC

López-Cervantes

Sánchez-Machado

Gutiérrez-Coronado

et al. 2006

Biomedical Chromatography

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“…A presença do pico base m/z 269 foi utilizada para caracterizar o retinol (LI et al,2005;WANG et al, 2001). E, nesse caso, a presença dos íons m/z 213, 177 e 95 corroboram a estrutura do retinol.…”

Section: Referênciasunclassified

“…O mesmo íon foi observado em estudo realizado por Heudi, Trisconi e Blake (2004) analisando retinol em fórmulas infantis enriquecidas. Em trabalho realizado por Li et al (2005), novamente, o íon majoritário m/z 269 foi observado após perda de água ([m+H)-H2O]+269).…”

Section: Referênciasunclassified

Obtenção De Padrão Analítico De Retinol a Partir De Fígado Bovino Por Cromatografia Líquida De Alta Eficiência

et al. 2017

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RESUMOPara a quantificação de substâncias de interesse, por Cromatografia Líquida de Alta Eficiência (CLAE), é necessário a obtenção de padrões analíticos confiáveis. Porém a aquisição destes padrões, com certificado de garantia, quase sempre depende de importação e apresenta custos elevados. As substâncias adquiridas ainda podem sofrer degradação, devido ao longo tempo de transporte e as condições inadequadas de armazenamento. A vitamina A ou retinol (C 20 H 30 O) é um álcool lipossolúvel encontrada apenas em fontes animais, em especial em áreas de armazenamento como o fígado ou associadas a gorduras como a do leite. O presente trabalho teve como objetivo isolar e quantificar retinol com alto teor de pureza a partir de fígado bovino para posterior utilização como padrão

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“…But electrochemical reactions in the ESI source can be a positive factor when used in a controlled way to intentionally affect the ESI-MS analysis of the analytes of interest. An important example is the direct electrochemical transformation of neutral, relatively nonpolar analytes to reasonably stable ionic species that can be detected in ESI-MS [9][10][11][12][13][14][15]. In addition, initial products of the electrochemical reaction may be used to react with the analyte of interest to form follow-up reaction products that can be used to analytical advantage.…”

Section: Introductionmentioning

confidence: 99%

Utilizing the inherent electrolysis in a chip-based nanoelectrospray emitter system to facilitate selective ionization and mass spectrometric analysis of metallo alkylporphyrins

Berkel

Kertész

2012

Anal Bioanal Chem

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A commercially available chip-based infusion nanoelectrospray ionization system was used to ionize metallo alkylporphyrins for mass spectrometric detection and structure elucidation by mass spectrometry. Different ionic forms of model compounds (nickel (II), vanadyl (II), copper (II), and cobalt (II) octaethylporphyrin) were created by using two different types of conductive pipette tips supplied with the device. These pipette tips provide the conductive contact to solution at which the electrolysis process inherent to electrospray takes places in the device. The original unmodified, bare carbon-impregnated plastic pipette tips were exploited to intentionally electrochemically oxidize (ionize) the porphyrins to form molecular radical cations for detection. Use of modified pipette tips, with a surface coating devised to inhibit analyte mass transport to the surface or slow the kinetics of the analyte electrochemical reactions, was shown to limit the ionic species observed in the mass spectra of these porphyrins largely, but not exclusively, to the protonated molecule. Under the conditions of these experiments, the effective upper potential limit for oxidation with the uncoated pipette tip was 1.1 V or less, and the coated pipette tips effectively prevented the oxidation of analytes with redox potentials greater than about 0.25 V. Product ion spectra of either molecular ionic species could be used to determine the alkyl chain length on the porphyrin macrocycle. The utility of this electrochemical ionization approach for the analysis of naturally occurring samples was demonstrated using nickel geoporphyrin fractions isolated from Gilsonite bitumen. Acquiring neutral loss spectra as a means to improve the specificity of detection in these complex natural samples was also illustrated.

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Determination of carotenoids and all-trans-retinol in fish eggs by liquid chromatography–electrospray ionization–tandem mass spectrometry

Cited by 63 publications

References 29 publications

Quantification of astaxanthin in shrimp waste hydrolysate by HPLC

Quantification of astaxanthin in shrimp waste hydrolysate by HPLC

Obtenção De Padrão Analítico De Retinol a Partir De Fígado Bovino Por Cromatografia Líquida De Alta Eficiência

Utilizing the inherent electrolysis in a chip-based nanoelectrospray emitter system to facilitate selective ionization and mass spectrometric analysis of metallo alkylporphyrins

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