Determination of Carbon-14 in Aqueous Bicarbonate Solutions by Liquid Scintillation Counting Techniques.  Application to Biological Fluids

Bruno, G.; Christian, John E.

doi:10.1021/ac60177a025

Cited by 454 publications

(106 citation statements)

References 6 publications

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“…B ) Urine was collected in 5% acetic acid for l2hr and the radioactivity was determined by the method of Bruno and Christian. 9 ) The animals were killed by decapitation l2hr after the administration of the isotopes. The liver was removed, and shed blood was combined with the carcass.…”

Section: Animals and Dietsmentioning

confidence: 99%

Metabolism of Arginine, Proline and Glutamic Acid in Growing Rats at Various Dietary Protein Levels

Tanaka

Ohyama

Ogura

1984

Agricultural and Biological Chemistry

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Section: Animals and Dietsmentioning

confidence: 99%

Metabolism of Arginine, Proline and Glutamic Acid in Growing Rats at Various Dietary Protein Levels

Tanaka

Ohyama

Ogura

1984

Agricultural and Biological Chemistry

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confidence: 99%

Metabolism of histidine in growing rats at various dietary protein levels.

Tanaka

Ogura

1980

Agricultural and Biological Chemistry

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The metabolic fate of the carbon skeleton of L-(U-34C)-histidine has been investigated in growing rats fed diets containing different percentages of protein calories (0,5,10, 15 and 30 PC%) at 410 kcal of metabolizable energy per 100g diet.The incorporation of 14C into body protein at 12 hr after injection of 14C-histidine was about 70% of the dose in rats fed 0 to 10 PC% diets, though the value was reduced in rats fed higher PC% diets. The expired 14C02 production was depressed in the low protein groups, and it showed an inverse pattern to that of 14C incorporation into body protein. Urinary excretion of 14C was about 20% of the dose in all dietary groups. The activities of hepatic histidase, urocanase and histidinepyruvate aminotransferase were increased in the 30 PC% group.These results indicate that the metabolic response of histidine to dietary protein changes around 10 PC%, where growth rate and body protein retention reached approximate plateaus.The nutritional significance of the metabolism of histidine has been discussed and compared with that of leucine, alanine and serine reported previously.

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“…Uptake treatments were terminated by transferring root segments from the influx medium to 50 ml of ice-cold 0.25 H for 5 min prior to filtration with a Buchner funnel. The root segments were then placed in scintillation vials and counted in 15 ml of scintillation fluid containing 60 g/l of Cabosil (1).…”

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confidence: 99%

Cadmium Alteration of Root Physiology and Potassium Ion Fluxes

Keck¹

1978

Plant Physiol.

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Segments of oat ( Avens sativa L.) roots which had been exposed to 1 nillimolar CdSO4 in quarter-strength Hoagland No. 1 solution exhibited decreased respiratory rates, ATP levels, membrane-bound ATPase activity, and reduced K+ fluxes. Respiration and ATP levels were decreased after a 2-hour treatment with 1 millimolar CdSO4 to 65 and 75%, respectively, of control rates. A membrane-bound, Mg2e-dependent, K+- Several studies have been published which assess the toxicity of Cd2" to selected plant species by determination of growth inhibition, cadmium content, and symptomologJr. These studies have indicated species-variable tolerances to Cd + added to either soil (7, 13) or hydroponic culture (18,22). Cadmium has been shown to enter barley roots by diffusion (3). The published results suggest that root plasmalemmas are the primary barrier to Cd2+ uptake and that growth may cease prior to a large accumulation of Cd2+ in the shoots (7,22). Previous work with mitochondria isolated from animal cells suggests that Cd2+ uncouples oxidative phosphorylation (11,17) and blocks electron transport (17). Cadmium also inhibits plasma membrane and mitochondrial ATPases isolated from animal cells (17).Some ionic fluxes into roots require expenditure of energy by the cell. While the primary source of energy required for energydependent ionic fluxes is unknown, the two extant hypotheses are: (a) redox-linked reactions possibly of the electron transport chains, (2,20); and (b) ATP supplied energy to plasma membrane-bound ATPases (10).The work reported here is concerned with the root plasma membrane, the initial site of Cd2+ action on plants. The

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Determination of Carbon-14 in Aqueous Bicarbonate Solutions by Liquid Scintillation Counting Techniques. Application to Biological Fluids

Cited by 454 publications

References 6 publications

Metabolism of Arginine, Proline and Glutamic Acid in Growing Rats at Various Dietary Protein Levels

Metabolism of Arginine, Proline and Glutamic Acid in Growing Rats at Various Dietary Protein Levels

Metabolism of histidine in growing rats at various dietary protein levels.

Cadmium Alteration of Root Physiology and Potassium Ion Fluxes

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