Determination of Calcium in Urine and Serum by Atomic Absorption Spectrophotometry (AAS)

Trudeau, David; Freier, Esther F.

doi:10.1093/clinchem/13.2.101

Cited by 237 publications

(31 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Aliquots were taken from each specimen passed and stored at -20°C until analysis. Urinary content of calcium was determined using a carrier gf aqueous solution of lanthanum (Willis, 1961 ;Trudeau and Freier, 1967) in the Pye Unicam SP 191 single beam atomic absorption spectrophotometer linked to an autosampler and external digital printer. Blank solutions and control standards were interspersed among the subject samples.…”

Section: Methodsmentioning

confidence: 99%

The Influence of Refined Carbohydrate on Urinary Calcium Excretion

Thom

Morris

et al. 1978

British Journal of Urology

View full text Add to dashboard Cite

In the course of study of the influence of varying the amount of refined carbohydrate (sugar and sugar products) in an otherwise standardised diet in 18 normal subjects it was evident that the analysis of 24-h urine collections failed to show the profound diurnal variation in urinary electrolyte excretion and, in particular in this instance, calcium excretion. The analysis of individually voided specimens showed some normal subjects to have spontaneously high peaks of urinary calcium concentration throughout the day even whilst on a diet with low refined carbohydrate content. Increase in the refined carbohydrate content of the otherwise standardised diet caused significant increase in the number of urines with a calcium concentration above 9 mmol/1. Refined carbohydrate, a common cinstituent in Western diets, can therefore influence urinary electrolyte excretion in such a way that there may be an increased risk of over-saturation with calcium oxalate.

show abstract

Section: Methodsmentioning

confidence: 99%

The Influence of Refined Carbohydrate on Urinary Calcium Excretion

Thom

Morris

et al. 1978

British Journal of Urology

View full text Add to dashboard Cite

show abstract

“…The test area was ter, the tubes were centrifuged for 15 min at marked by using a Lettraset tape, and the control 9500 X g, and the Ca+ * concentradon in the super-areas were lacquered with nail varnish for protecnatant was measured using atomic absorpdon tion. Thus, a study window of 1 x 3 mm was obspectrophotometry (10).…”

Section: Calcium Release Itt Vitro -Five Mg Hydroxyapadtementioning

confidence: 99%

Experimental sports drinks with minimal dental erosion effect

Meurman

Härkönen

Näveri

et al. 1990

European J Oral Sciences

View full text Add to dashboard Cite

– The effects of new experimental sports drinks on dental enamel were studied in vitro using bovine tooth specimens. Profilometric analysis was used to measure the loss of tooth material after immersion of the specimens in the drinks. Thereafter the specimens' surface hardness was measured and scanning electron microphotographs were taken. In addition, 13 commercial sports drinks and experimental drinks containing either citric acid or malic acid were tested for their capacity to dissolve hydroxyapatite in vitro. The erosive effect increased markedly with decreasing pH. The citric acid containing drinks were more erosive than malic acid containing drinks. No erosion was observed with the malic acid containing drink (pH 5.90) but the drink of similar composition containing citric acid caused an erosion 1.3± 1.1μm deep and a commercial citric acid containing drink caused a lesion 12.3± 4.5μm deep after 120 min immersion. Softening of enamel was greater in specimens immersed in citric acid than in those immersed in malic acid containing drink. The in vitro hydroxyapatite dissolving effect of the commercial sports drink samples studied (all having a pH under 4.22) was markedly greater (0.48–4.38 mmol/l) than that of the malic acid containing experimental drink (pH 5.50, Ca++ concentration in the supernatant 0.19 mmol/l) and of the similar citric acid containing drink (0.35 mmol/l). The hydroxyapatite dissolving effect of both drinks started to be marked at a pH level of about 5.0 but increased thereafter exponentially with decreasing pH. At pH levels above 4.0 the hydroxyapatite dissolving effect of citric acid containing drinks was greater than that of malic acid containing drinks.

show abstract

“…Total hydroxyproline in urine was determined by the method of Gordeladze, Halse, Dj0seland & Haugen (1978). Calcium and magnesium in feedstuffs and in plasma were estimated by atomic absorption spectrophotometry by modifications of the methods of Trudeau & Freier (1967). Inorganic phosphorus (Pi) concentrations in plasma and phosphorus in feedstuffs were determined by the method of Quinlan & DeSesa (1955).…”

Section: Biochemistrymentioning

confidence: 99%

Assessment of bone turnover in the dry period of dairy cows by measurement of plasma bone GLA protein, total plasma alkaline phosphatase activity and urinary hydroxyproline

Mosel¹,

Corlett²

1990

Experimental Physiology

View full text Add to dashboard Cite

SUMMARYPlasma osteocalcin, or plasma bone GLA protein (BGP), total plasma alkaline phosphatase activity and urinary hydroxyproline excretion of twenty-four pregnant dairy cows (thirteen in their first or second pregnancy, i.e. low parity, and eleven in their third or more pregnancies, i.e. high parity) were measured from 7 weeks before parturition until 1 week after parturition. Seven weeks before parturition the cows' ration was changed to one containing either 0-22 % magnesium (low magnesium, LMg) or 0-82 % magnesium (high magnesium, HMg) in the dry matter, and the potassium content of both rations was increased to approximately 4-1 % in the dry matter to reduce the absorption of magnesium. Plasma BGP levels decreased significantly (P < 0-01) as parturition approached while total plasma alkaline phosphatase activity and urinary hydroxyproline excretion did not. Magnesium supply and parity had no significant effect on this decrease. The overall plasma concentration of BGP, total plasma alkaline phosphatase activity and the urinary hydroxyproline/creatinine ratio in the prepartum period were affected by parity (P < 0 05) with higher values in the lower parity cows. A significant positive correlation (r = 0 58, P < 0-01) was found in all cows between plasma BGP level at parturition and the percentage of the bone surface covered with osteoblasts; however, plasma BGP was not correlated either with other histomorphometric variables or with total alkaline phosphatase activity during this time.

show abstract

Determination of Calcium in Urine and Serum by Atomic Absorption Spectrophotometry (AAS)

Cited by 237 publications

References 0 publications

The Influence of Refined Carbohydrate on Urinary Calcium Excretion

The Influence of Refined Carbohydrate on Urinary Calcium Excretion

Experimental sports drinks with minimal dental erosion effect

Assessment of bone turnover in the dry period of dairy cows by measurement of plasma bone GLA protein, total plasma alkaline phosphatase activity and urinary hydroxyproline

Contact Info

Product

Resources

About