Determination of bulleyaconitine A in human plasma by liquid chromatography–electrospray ionization tandem mass spectrometry

Sensitive Quantification of Bulleyaconitine A in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry

“…The concentration of BLA was 0.235 ng/ml. Moreover, the serum required was only 0.2 ml, about 1/5 of that reported in the previous literature, 11) and it provided higher sensitivity. The small sample volume required by our method should alleviate the discomfort of participating patients.…”

“…Liquid chromatography-tandem mass spectrometry (LC/MS/MS) is an attractive method that has markedly improved detection sensitivity for drugs, and quantification on the order of picograms, is becoming possible. Weng et al 11) have reported a highly sensitive LC/MS/MS method to determine the plasma BLA concentration. Unfortunately, the method involves using a large volume of plasma and a laborious liquid-liquid extraction procedure.…”

Recent applications of liquid chromatography–mass spectrometry in natural products bioanalysis

“…Flavonoids in biofluids were usually extracted by ethyl acetate after acidification [94][95][96], whereas alkaloids were usually extracted by chloroform or ether after alkalification [97][98][99][100][101][102][103]. Saponins and sesquiterpenoids were mainly extracted by n-butanol, methylene chloride, ether or ethyl acetate [104][105][106][107][108].…”

Simultaneous Determination of 17 Toxic Alkaloids in Human Fluids by Liquid Chromatography Coupled With Electrospray Ionization Tandem Mass Spectrometry

“…In addition, HPLC usually can't reach the sensitivity demanded for poisoned biological samples, especially when the concentration of the analytes is so low that the background noise may cause interference in the appearance of peaks of analytes. Nowadays, liquid chromatography-mass spectrometry (LC-MS) techniques have become more common for the detection of alkaloids in different samples, [23][24][25] especially in biological fluids. With the multiple-reaction monitoring (MRM) mode which can effectively reduce background noise and increase signal to noise ratio, the selectivity and sensitivity of this method are greatly improved.…”