Recent applications of liquid chromatography–mass spectrometry in natural products bioanalysis

Xing, Jie; Xie, Chunfeng; Lou, Hongxiang

doi:10.1016/j.jpba.2007.01.010

Cited by 81 publications

(46 citation statements)

References 161 publications

(226 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are quite consistent with previous publications in which flavonoids are reported to be extensively degraded by the intestinal microflora or enzymes in vivo. [6][7][8][9][10][11][17][18][19] …”

Section: Metabolic Study Of Flavonoid Extract Of Jujube Seeds In Rat mentioning

confidence: 99%

Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn)

Bao

et al. 2009

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

Semen Ziziphi Spinosae (jujube seeds), the dried semen of Ziziphus jujuba MILL. var. spinosa (BUNGE) HU ex H. F. CHOU, is one of the most famous traditional Chinese medicines (TCMs) with hypnosis, sedation, and anticonvulsivant effects.1) It has been widely used for the treatment of insomnia in clinical practice.2) Chemical investigations on jujube seeds resulted in the discovery of several groups of bioactive components including flavonoids, saponins, and fatty acids. 3,4) Modern pharmacological studies have demonstrated that flavonoids are the main bioactive compounds responsible for the sedative and hypnotic effects of jujube seeds. 2,5) Hence it is important to explicate the biotransformation of flavonoids in vivo so as to clarify the mechanism of pharmacological action and to promote its availability as well. To date, there are few reports regarding the physiological disposition of flavonoids after oral administration of jujube seeds-containing TCMs 6,7) ; however, the biotransformation of flavonoids in vivo remains unknown. The challenges for the metabolic study in vivo of TCMs extract lie in two aspects: one is the complexity of TCMs containing hundreds of constituents, and the other is the lack of proper analytical methods for the identification of the metabolites even in trace amount. 8)A major goal of this study was to develop a highly specific and sensitive high-performance liquid chromatography method coupled with diode-array detection and ion-trap mass spectrometry (HPLC-DAD-MS n ) for the study of the constituents and metabolites in rat plasma, urine, and feces after oral administration of flavonoid extract of jujube seeds, providing scientific plausibility for the activity of this extract. These achievements would support preclinical pharmacokinetic studies and achieve a better understanding of the pharmacological action mechanism of flavonoid extract of jujube seeds. ExperimentalMaterials Jujube seeds were purchased from a genuine area Xintai (Hebei, China) and identified by one of the authors, Dr. Ping Li. Spinosin (with a purity of 99.0%), and 6ٞ-feruloylspinosin (with a purity of 99.0%) (Fig. 1) was prepared in our laboratory. Male Sprague-Dawley (SD) rats were acquired from the Laboratory Animal Center of China Pharmaceutical University (Nanjing, China). HPLC grade acetonitrile was purchased from Merck KGaA Company (Merck, Germany). Deionized water was purified by Milli-Q system (Millipore, U.S.A.). Physiological saline was purchased from Shijiazhuang Pharmaceutical Corporation (Shijiazhuang, China). Heparin sodium, obtained from Hanbang Science & Technology (Nanjing, China), was dissolved in physiological saline at a concentration of 1250 U/ml and used to rinse the test tubes prior to blood collection for plasma. Glacial acetic acid, as well as the other reagents, was of analytical grade.Preparation of Flavonoid Extract from Jujube Seeds Jujube seeds (3.0 kg) were ground into powder (particle size 20-40 mesh) and refluxed with 24 l of petroleum ether (60-90°C) three times (each for 12 h). Af...

show abstract

Section: Metabolic Study Of Flavonoid Extract Of Jujube Seeds In Rat mentioning

confidence: 99%

Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn)

Bao

et al. 2009

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

show abstract

“…Consequently, developing and optimizing analytical methods for identification of metabolites of natural products in biological samples is of significance to a safe and optimal use of herbal products. LC-MS plays a prominent role as the analytical tool for detection and identification of pharmacologically active metabolites and/or of reactive metabolites [55]. The constant neutral loss (CNL) and precursor ion scanning (Prec) have been widely used for the screening of glucuronide or sulfate conjugates derived from reactive metabolites in phase II [62].…”

Section: Qualitative Analysismentioning

confidence: 99%

“…Moreover, new metabolites generated during metabolism can possess unknown properties. For these reasons, careful examination of metabolites in biological systems is required [55,64]. Guo et al investigated phase I metabolites of isoliquiritigenin (2 ,4 ,4 -trihydroxychalcone) using human liver microsomes on a Q-TOF hybrid mass spectrometer (Waters Micromass Q-TOF-2) [100].…”

Section: Phenols and Flavonoidsmentioning

confidence: 99%

“…Besides, the hyphenations of LC-MS with other separation or analytical techniques have frequently been applied in natural products analysis. Several review articles dealing with LC-MS and its application in the analysis of natural products have been published [54][55][56][57][58][59]. With the aim of providing an up-to-date overview of LC-MS applications on the phytochemical analysis, the present review summarizes recent development of identification and measurement of phytochemical constituents and their metabolites, as well as fingerprint analysis for herbal medicines during the past few years (2007)(2008)(2009)(2010)(2011)(2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

160

View full text Add to dashboard Cite

“…Typically, natural products can be analyzed using various detection techniques including ultraviolet or photodiode array, fluorescence, evaporative light scattering, and mass spectrometric detection [11][12][13][14][15]. Highperformance liquid chromatography (HPLC) with ultraviolet (UV) and photodiode array (PDA) detection methods has become a routine practice in some laboratories to quantify benzenoids and triterpenoids derivatives of fruiting body, mycelia, extract, and commercial preparation of AC [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative analysis of seven signature components in the fruiting body of Antrodia cinnamomea by HPLC—ESI—MS/MS

Huang

Hsu

Chen³

et al. 2016

Acta Chromatographica

View full text Add to dashboard Cite

Summary. An accurate and rapid liquid chromatography-electrospray ionizaiontandem mass spectrometry (LC-ESI-MS/MS) analytical method was developed and validated for the simultaneous determination of antcins A, B, C, H, and K, dehydroeburicoic acid, and 4,7-dimethoxy-5-methyl-1,3-benzodioxole in the extract and capsule of Antrodia cinnamomea (AC) fruiting body. These seven signature compounds were ionized using an electrospray ion source and analyzed by a triple-quadrupole mass analyzer under a multiple reaction monitoring ( , and m/z 197/139 (4,7-dimethoxy-5-methyl-1,3-benzodioxole) were used to quantify these seven components, respectively. Their calibration curves presented good linear regressions (R 2 > 0.997) within the tested concentration range. The intra-and inter-day precisions were less than 1.97% and 2.53%, respectively. The overall recovery was in the range of 87.55%-95.41%. This validated high-performance liquid chromatography (HPLC)-MS/MS method offers promising applications for the accurate and rapid quantification of signature compounds in the fruiting body and its commercial products.

show abstract

Recent applications of liquid chromatography–mass spectrometry in natural products bioanalysis

Cited by 81 publications

References 161 publications

Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn)

Characterization of Flavonoid Metabolites in Rat Plasma, Urine, and Feces after Oral Administration of Semen Ziziphi Spinosae Extract by HPLC-Diode-Array Detection (DAD) and Ion-Trap Mass Spectrometry (MSn)

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Qualitative and quantitative analysis of seven signature components in the fruiting body of Antrodia cinnamomea by HPLC—ESI—MS/MS

Contact Info

Product

Resources

About