Determination of boron in biological tissues by inductively coupled plasma optical emission spectrometry (ICP-OES)

Boron Determination—A Review of Analytical Methods

“…Development of ICP-OES revolutionized the determination of several so-called ''problem elements'' such as B, S, Mo, and all hard to detect trace elements by virtue of its low detection limits, large linear range, and multielement (several elements in the same run) detection capability. Reported detection limits for B are 10 to 15 mg B/L in soil solutions and plant digests by ICP-OES based on a linear self-scanning photodiode array (51), 0.1 ppm (100 mg B/L) in as little as 50 mg animal tissues (tumor, blood, liver, skin), or as few as 5 1 10 7 blood cells in cell suspensions by DCP-OES (132), and 25 mg B/L in the microwave digest of mice tissues using Ar-ICP-OES with a Babington nebulizer (44). Some examples of the applications of the ICP-OES method for B determination in various sample types are presented in Table 2.…”

“…Examples of these methods include spectrophotometric (32,43), ICP-OES (44,45), and ICP-MS methods (46 -48). The microwave dissolution is also used for the decomposition of nonbiological materials such as steel samples (6) using diluted aqua regia (3HCl:1HNO 3 v:v) and for coal ash samples (49) using HCl/HNO 3 /HF.…”

Subcellular localization of boron in cultured melanoma cells by electron energy‐loss spectroscopy of freeze‐dried cryosections

“…Owing to the inhomogeneous distribution of the 10 B‐compounds at microscopic level, the results of such measurements integrated over a bigger volume than the irradiated volume cannot predict reliably the radiation effect. However, to evaluate different boron uptake in several tissues and tumours, inductively coupled plasma (ICP) atomic emission spectrometry (ICP‐AES), mass spectrometry (ICP‐MS) or laser ablation‐ICP‐MS are widely used to obtain a rapid and sensitive determination of mean concentration of boron in biological samples (Pollmann et al ., 1993; Probst, 1999). Secondary ion mass spectroscopy (SIMS) or high‐resolution alpha autoradiography are also used for imaging boron distributions at the cellular level (Solares & Zamenhof, 1995; Smith et al ., 2001).…”

Pharmacokinetic evaluation and antitumor potency of liposomal nanoparticle encapsulated cisplatin targeted to CD24‑positive cells in ovarian cancer

“…Two samples of disseminated tumors, liver and kidney were prepared from each animal. Absolute Platinum (Pt) concentrations in the tissue samples were measured by ICP-OES (Vista-MPX ICP-OES spectrometer, Seiko Instruments) as described previously (23). For the standardization of equipment and measurements, Spectro multi-element and Spectrum 3D standards were used (24).…”