Determination of Biotin and Folate in Infant Formula and Milk by Optical Biosensor-Based Immunoassay

Abstract

Biomolecular interaction analysis was evaluated for the automated analysis of biotin- and folate-supplemented infant formulas and milk powders. The technique was configured as a biosensor-based, nonlabeled inhibition immunoassay using monoclonal antibodies raised against analyte-conjugate. Sample extraction conditions were optimized and antibodies were evaluated for cross-reactivity. Performance parameters included a quantitation range of 2–70 ng/mL, recoveries of 86–102%, agreement against assigned reference values for National Institute of Standards and Technology Standard Reference Material 1846, between-laboratory reproducibility relative standard deviation of 9.1% for biotin and 8.1% for folate, respectively, and equivalence against reference microbiological assay methods for both analytes.

Evaluation of Micronutrient Sensors for Food Matrices in Resource‐Limited Settings: A Systematic Narrative Review

“…After independent full-text review, the reviewers met to exclude articles (n = 41) after reaching a final agreement for the following reasons: matrix is not food or potable water (n = 32), no interference study was conducted (n = 2), no dynamic range or limit of detection were studied (n = 4), and previously reported sensors in prior studies (n = 3). A total of 42 sensors (Aksuner, Henden, Yenigul, Yilmaz, & Cukurovali, 2011;Alexander, Dimitrakopoulos, & Hibbert, 1997 Hosseini et al, 2010;Indyk et al, 2000;Indyk et al, 2002;L. Kong et al, 2016;Kumar et al, 2014;Lermo et al, 2009;W.…”

Immunoassays: their history, development and current place in food science and technology

“…Examples presented in this issue include allergens (Immer et al, 2004), hormones (Johansson & Hellenas, 2004) and drug residues (Myrtazina et al, 2004). Other analytes in food for which immunoassays have been developed include pesticides (Bonwick et al, 1996b), plant gums (Thurston et al, 1998), vitamins (Indyk et al, 2000), fungal toxins ) and pathogens (de Boer & Beumer, 1999). Tests for food processing such as irradiation have also been reported (Williams et al, 1994).…”

Survey of the year 2000 commercial optical biosensor literature

“…One of the most rapidly expanding areas of biosensor use is quality control, contaminant detection and concentration analysis in the food/dairy industries and environmental settings. For example, biosensors are being used to quantitate nutrient and antibiotic levels in foodstuffs, 392,395,405,406 bacterial and fungal contamination in food, 32,398 and air-and water-borne toxins, pesticides and herbicides. 212,410,426 The on-site, on-line capabilities of biosensors, coupled with the technology's high sensitivity and miniscule sample requirements, make these instruments ideal tools to monitor food and environmental safety.…”