Determination of biothiols by a novel on-line HPLC-DTNB assay with post-column detection

Özyürek, Mustafa; Baki, Sefa; Güngör, Nilay; Çelik, Saliha Esin; Güçlü, Kubilay; Apak, Reşat

doi:10.1016/j.aca.2012.03.056

Cited by 63 publications

(18 citation statements)

References 27 publications

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“…On-line HPLC-UV-ABTS method has been shown to be superior in sensitivity, selectivity, and relative simplicity for antioxidant capacity analysis, and applied for quick identification of antioxidant components of various plant extracts, foods, and beverages (He et al, 2010;Pellegrini, Rio, Colombi, Bianchi, & Brighenti, 2003). Among the increasing number of methods available to determine the antioxidant capacity of food extracts are online HPLC-FRAP assay (Burnaz, 2013;Raudonis, Raudone, Lakstas, & Janulis, 2012), online HPLC-CUPRAC assay (Celik, Ozyürek, Güclü, & Apak, 2010), online HPLC-DTNB assay (Ozyürek et al, 2012), and enzyme-based online assay (Quaranta et al, 2013).…”

Section: Introductionmentioning

confidence: 98%

Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods

et al. 2015

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Section: Introductionmentioning

confidence: 98%

Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods

et al. 2015

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“…The choice of derivatization either in pre-or post-column modes is one of the most widely used techniques, since it improves not only stabilization of biothiols but also chromatographic properties. For example, reverse-phase liquid chromatography (RPLC) has been employed in concert with various detectors -such as fluorescence, UV and inductively coupled plasma mass spectrometry (ICP-MS) detection -following derivatization [13][14][15]. However, the majority of these methods are tedious and time-consuming due to complex derivatization steps.…”

Section: Introductionmentioning

confidence: 99%

A novel method for the simultaneous analysis of seven biothiols in rice (Oryza sativa L.) using hydrophilic interaction chromatography coupled with electrospray tandem mass spectrometry

Cao

Sun

Mou

et al. 2015

Journal of Chromatography B

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“…Many analytical techniques have been employed for quantitative determination of PCs. One of the most important assays is HPLC separations in concert with fluorescence or UV detection, followed by derivatization . A big advantage of derivatization is the prevention of thiol oxidation during analysis.…”

Section: Introductionmentioning

confidence: 99%

Robust method for the analysis of phytochelatins in rice by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry based on polymeric column materials

Bian

Zhou

et al. 2015

J. Sep. Science

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A sensitive and robust high-performance liquid chromatography coupled with electrospray tandem mass spectrometry method for the identification and quantification of glutathione and phytochelatins from rice was developed. Homogenized samples were extracted with water containing 100 mM dithiothreitol, and solid-phase extraction using polymer anion exchange resin was employed for sample purification. Chromatography was performed on a polymeric column with acetonitrile and water containing 0.1% formic acid as the mobile phase at the flow rate of 300 μL/min. The limit of quantitation was 6-100 nM. This assay showed excellent linearity for both glutathione and phytochelatins over physiological normal ranges, with correlation coefficients (r) > 0.9976. Recoveries for four biothiols were within the range of 76-118%, within relative standard deviations less than 15%. The intraday precision (n = 7) was 2.1-13.3%, and the interday precision over 15 days was 4.3-15.2%. The optimized method was applied to analyze tissue samples from rice grown using nutrient solutions with three different cadmium concentrations (0, 50, and 100 μM). With increasing cadmium concentrations, the content of phytochelatin 2 and phytochelatin 3 in rice roots increased, in contrast to most phytochelatins, and the content of glutathione in rice stems and roots decreased significantly.

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Determination of biothiols by a novel on-line HPLC-DTNB assay with post-column detection

Cited by 63 publications

References 27 publications

Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods

Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods

A novel method for the simultaneous analysis of seven biothiols in rice (Oryza sativa L.) using hydrophilic interaction chromatography coupled with electrospray tandem mass spectrometry

Robust method for the analysis of phytochelatins in rice by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry based on polymeric column materials

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