Determination of Bioavailable Testosterone [Non–Sex Hormone–Binding Globulin (SHBG)-Bound Testosterone] in a Population of Healthy French Men: Influence of Androstenediol on Testosterone Binding to SHBG

Giton, Frank; Urien, Saı̈k; Born, Catherine; Tichet, Jean; Guéchot, Jérôme; Crinon, Jacques; Bronsard, Françoise; Raynaud, Jean; Fiet, Jean

doi:10.1373/clinchem.2007.087312

Cited by 35 publications

(42 citation statements)

References 41 publications

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“…Indeed, 5-diol has a direct androgenic action, as reported (27), since it can activate the androgen receptor without being metabolized into TT, and has an indirect androgenic effect, since it increases SHBGunbound TT and BT (20).…”

Section: Discussionmentioning

confidence: 95%

“…The significantly higher assayed BT in African-Caribbeans is consistent with the higher 5-diol levels we found in them. 5-diol has an SHBG association constant similar to that of TT (26) and contributes to the displacement of plasma TT from binding to SHBG (20), consequently to the increase in SHBG-unbound TT and BT in African-Caribbeans. We found higher 4-dione and 5-diol levels in AfricanCaribbean men compared with Europeans.…”

Section: Discussionmentioning

confidence: 99%

“…Bioavailable testosterone (BT) was measured by addition of purified tritiated TT to the serum samples, incubating and then precipitating the sex hormone-binding globulin (SHBG)-bound TT with saturated ammonium sulfate (20,24). After centrifugation, we deduced the percentage of SHBG-unbound tritiated TT (BT%), and multiplying by total TT concentration, we obtained the serum BT concentration.…”

Section: Assay Method/ Analytesmentioning

confidence: 99%

“…Caribbean subjects of African descent (African-Caribbeans) were recruited in Les Abymes, the biggest city in Guadeloupe (French West Indies), a Caribbean archipelago in which most inhabitants are of African descent (19). European subjects of Caucasian descent (Europeans) were recruited in Tours, the biggest city in the Indre-et-Loire Province (mainland France) (20). Subjects were selected according to the birthplaces of their parents as follows: any Caribbean island the population of which is predominantly of African descent for African-Caribbean subjects and Western Europe for European subjects.…”

Section: Populationmentioning

confidence: 99%

“…DHEA, D4-androstenedione (4-dione), D5-androstenediol (5-diol), testosterone (TT), dihydrotestosterone (DHT), estrone (E1), estradiol (E 2 ), and E1 sulfate (E1-S) were assayed simultaneously by MS coupled with GC on 1000 ml serum (20,22,23). Briefly, deuterated steroid internal standards (CDN Isotopes, Inc., Point-Claire, Quebec, Canada) were added to all serum samples and extracted with 1-chlorobutane.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

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Serum sex steroids measured in middle-aged European and African–Caribbean men by gas chromatography–mass spectrometry

Giton

Fiet

Cornu³

et al. 2011

European Journal of Endocrinology

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Background: Differences in circulating steroid hormone levels have been hypothesized to explain ethnic differences in steroid-related diseases. The aim of this study was to determine the serum levels of a wide panel of steroid hormones, both androgens and estrogens, in healthy middle-aged African-Caribbean and European men. Design and methods: Serum steroid hormone levels were determined in men participating in a systematic public health study funded by the French National Health Insurance system. Blood was collected in the morning from 304 healthy African-Caribbean and European men aged between 40 and 69 years. Serum steroids were measured by mass spectrometry-gas chromatography, except for DHEAS and sex hormone-binding globulin, which were determined by RIA. Data were analyzed in 10-year age intervals by analysis of covariance, with adjustment for age, body mass index, waist-to-hip ratio, tobacco and alcohol consumption, and season of sampling. Results: Compared with Europeans, African-Caribbean men presented significantly higher serum levels of measured bioavailable testosterone, 4-androstenedione (4-dione), and estrone (E1) regardless of the age group, of 5-androstenediol (5-diol) in those aged 40-49 and 50-59 years, and of testosterone (TT) and dihydrotestosterone in those aged 40-49 years. In contrast, European men aged 40-69 years showed significantly higher serum levels of DHEA and DHEAS. Conclusions: Significant differences in serum steroid hormone levels were observed in middle-aged African-Caribbean and European men. Whether such differences could contribute to ethnic differences in disease risk in adult men remains to be investigated. Some steroids, such as bioavailable TT, 4-dione, 5-diol, and E1, deserve particular attention.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Assay Method/ Analytesmentioning

confidence: 99%

Section: Populationmentioning

confidence: 99%

Section: Laboratory Proceduresmentioning

confidence: 99%

See 3 more Smart Citations

Serum sex steroids measured in middle-aged European and African–Caribbean men by gas chromatography–mass spectrometry

Giton

Fiet

Cornu³

et al. 2011

European Journal of Endocrinology

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Sexual steroids in serum and prostatic tissue of human non‐cancerous prostate (STERPROSER trial)

et al. 2017

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Sex steroids in serum and prostatic tissue of human cancerous prostate (STERKPROSER trial)

et al. 2018

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Background Currently, there is no consensus regarding the expected concentration levels of intra‐prostatic sex steroids in patients with Prostate Cancer (PCa). Our objective was to assess the concentration levels of sex steroids in prostatic tissue and serum, in two cohorts of patients with localized PCa or benign prostatic hyperplasia (BPH). Methods Between September 2014 and January 2017, men selected for radical cystectomy (for bladder cancer) or open prostatectomy (for BPH), and men selected for radical prostatectomy for localized PCa were included. Blood samples were collected at baseline before surgery, and steroid concentrations were assessed following the recommendations of the Endocrine Society. Intra‐prostatic samples were collected from fresh surgical samples, and assessed by gas chromatography and mass spectrometry (GC/MS). Permanova analysis was performed. Analyses were adjusted for age, prostate weight, and prostate‐specific antigen (PSA) level. Results A total of 73 patients (41 patients with PCa and 32 patients with BPH) were included in this study. Patients with PCa were younger, and had smaller prostate volumes with higher levels of PSA. The levels of Total Testosterone (TT), Di‐Hydro‐Testosterone (DHT), and Estradiol (E2) in the serum were not significantly different between PCa and BPH. In PCa tissue, TT concentrations were significantly lower (0.11 ng/g vs 0.47 ng/g, P = 0.0002), however its derivative E2 had significantly higher concentrations (31.0 ng/g vs 22.3 ng/g, P = 0.01). DHT tissue concentrations were not significantly different between the two groups (5.55 ng/g vs 5.42 ng/g, P = 0.70). Intra‐prostatic TT concentrations were significantly lower in the peripheral zone than in the central zone for the CaP group (0.07 ng/g vs 0.15 ng/g, P = 0.004). Conclusions Patients with PCa had lower intra‐prostatic TT and higher E2 concentrations levels compared to the patients with BPH. PCa seem to consume more TT and produce more E2, especially in the peripheral zone.

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Determination of Bioavailable Testosterone [Non–Sex Hormone–Binding Globulin (SHBG)-Bound Testosterone] in a Population of Healthy French Men: Influence of Androstenediol on Testosterone Binding to SHBG

Cited by 35 publications

References 41 publications

Serum sex steroids measured in middle-aged European and African–Caribbean men by gas chromatography–mass spectrometry

Serum sex steroids measured in middle-aged European and African–Caribbean men by gas chromatography–mass spectrometry

Sexual steroids in serum and prostatic tissue of human non‐cancerous prostate (STERPROSER trial)

Sex steroids in serum and prostatic tissue of human cancerous prostate (STERKPROSER trial)

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