Determination of Azithromycin in pharmaceutical dosage forms by Spectrophotometric method

Suhagia, Bhanubhai N.; Shah, Syed Muhammad Ismail; Rathod, IS; Patel, HM; Doshi, K. R.

doi:10.4103/0250-474x.25726

Cited by 25 publications

(11 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Measure the absorbance of diluted sample of azithromycin: β-CD tablets at 298 nm, using ultravioletvisible spectrophotometer. [16,17] …”

Section: Drug Contentmentioning

confidence: 99%

Untitled

Wadhwa¹

2017

AJP

View full text Add to dashboard Cite

Aim: To increase the solubility of azithromycin by formulating solid dispersion (SD) and then SD tablets (SDT) were prepared from the best formulation of SDs. Material and Methods: SDs were prepared using polyethylene glycol 6000 and β-cyclodextrin (β-CD) by solvent evaporation method. To investigate drug-excipient interaction and for selection of suitable excipient for formulation differential scanning calorimetry study was done, and each excipient was selected for formulation development only if it showed compatible results. Results and Discussion: Tablets were prepared by direct compression technique using hydroxypropylmethylcellulose (HPMC)-K 100 and guar gum in different concentrations. SDs were evaluated for drug content, in vitro dissolution profiles, and developed SDT were evaluated for various pharmaceutical characteristics, viz., hardness, friability, weight variation, thickness, drug content, and in vitro drug release. Conclusion: Study indicates that among various formulations SDT of azithromycin: β-CD (1:2) complexes prepared using HPMC and guar gum in 1:4 combination showed maximum drug release.

show abstract

“…Measure the absorbance of diluted sample of azithromycin: β-CD tablets at 298 nm, using ultravioletvisible spectrophotometer. [16,17] …”

Section: Drug Contentmentioning

confidence: 99%

Untitled

Wadhwa¹

2017

AJP

View full text Add to dashboard Cite

show abstract

“…AZI has been analyzed by spectrophotometric methods [3][4][5], high-perfor-mance liquid chromatography using fluorescence [6][7][8], electro-chemical using amperometric detection [9][10] and coulometric detection [11][12][13] and mass spectrometry detector [14][15][16][17] for quantification in bulk material and pharmaceutical dosage forms. Fluorescence detection requires complicated sample pretreatment involving pre-column derivatization of the analyte.…”

Section: Trideoxy-3-(dimethylamino)-β-d-xylo-]oxy}-1-oxa-6-azacyclopementioning

confidence: 99%

A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma

Younes

Elkady

2014

Eur. J. Chem.

View full text Add to dashboard Cite

A simple, rapid and sensitive LC-MS/MS method was developed and validated for the quantification of azithromycin (AZI) in human plasma. Sildenafil citrate was used as an internal standard. The analytes were extracted from human plasma samples by liquid-liquid extraction technique. The reconstituted samples were chromatographed on a reversed -phase column C18, 50 × 2.1 mm, 5 µm from Waters, by using a 80:20 (v:v) mixture of acetonitrile and 0.1% formic acid in water as the mobile phase at a flow rate of 0.5 mL/min. The calibration curve was linear (r 2 > 0.99) over the concentration range of 5.0-1500.0 ng/mL. The results of the intra-and inter-day precision and accuracy studies were within the acceptable limits. A run time of 0.5 min for each sample made it possible to analyze more than 400 plasma samples per day.

show abstract

“…As for AZT, it has been determined by high performance liquid chromatography (HPLC) [3][4][5][6], liquid chromatography-mass detection (LC-MS/MS) [7,8], ultra-performance liquid chromatography (UPLC) [9], micellar liquid chromatography (MLC) [10], spectroflourimetry [11] and UVVisible spectrophotometry [12][13][14][15][16][17] while ROX has been estimated by HPLC [18,19], LC-MS/MS [20], spectroflourimetry [21,22] and UV-Visible spectrophotometry [23].…”

Section: Introductionmentioning

confidence: 99%

“…Spectrophotometry is a very important technique in drug analysis due to its availability in most laboratories, simplicity and low cost. The developed methods have simple procedures and wider determination ranges, also they are rapid and convenient in comparison with the reported spectrophotometric methods [12][13][14]23,24], so can be applied in quality control laboratories for routine analysis. …”

Section: Introductionmentioning

confidence: 99%

Two spectrophotometric methods for the determination of azithromycin and roxithromycin in pharmaceutical preparations

2017

View full text Add to dashboard Cite

Two new and simple spectrophotometric procedures have been proposed and validated for estimation of two important macrolide antibiotics namely, azithromycin dihydrate and roxithromycin. Method I depends on complex formation between any of the two drugs and copper in acidic medium where the absorbances of the produced complexes are measured at 250 and 264 nm with linearity ranges of 1.0-100.0 and 2.0-130.0 µg/mL for the two drugs, respectively. Method II depends on the reaction of these drugs with N-bromosuccinimide forming a product which is yellow colored, measured at 264 and 278 nm, with linearity ranges of 2.0-140.0 and 3.0-160.0 µg/mL for azithromycin dihydrate and roxithromycin, respectively. The proposed methods were subjected to detailed validation procedure; moreover they were used for the estimation of the concerned drugs in their different dosage forms. Study of the reactions stoichiometry was carried out; furthermore, a reaction mechanism proposal was presented.

show abstract

Determination of Azithromycin in pharmaceutical dosage forms by Spectrophotometric method

Cited by 25 publications

References 4 publications

Untitled

Untitled

A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma

Two spectrophotometric methods for the determination of azithromycin and roxithromycin in pharmaceutical preparations

Contact Info

Product

Resources

About