Determination of avilamycin A and B in pig faeces by solid phase extraction and reverse-phase HPLC assay

Sunderland, J.; Lovering, Andrew; Tobin, C. M.; MacGowan, Alasdair; Roe, John M.; Delsol, Anne A.

doi:10.1016/j.ijantimicag.2004.06.009

Cited by 5 publications

(2 citation statements)

References 9 publications

(9 reference statements)

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“…The dissolving solution was passed through the column to clean, the mixture of chloroform: acetone (4:1) was used to wash, and eluted with a mixture of chloroform: acetone (3:7). The mixture was vortexed and analyzed by liquid chromatography ( Sunderland et al, 2004 ).…”

Section: Methodsmentioning

confidence: 99%

Optimal Regimens and Clinical Breakpoint of Avilamycin Against Clostridium perfringens in Swine Based on PK-PD Study

Huang¹,

Luo²,

Xu³

et al. 2022

Front. Pharmacol.

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Clostridium perfringens causes significant morbidity and mortality in swine worldwide. Avilamycin showed no cross resistance and good activity for treatment of C. perfringens. The aim of this study was to formulate optimal regimens of avilamycin treatment for C. perfringens infection based on the clinical breakpoint (CBP). The wild-type cutoff value (COWT) was defined as 0.25 μg/ml, which was developed based on the minimum inhibitory concentration (MIC) distributions of 120 C. perfringens isolates and calculated using ECOFFinder. Pharmacokinetics–pharmacodynamics (PK-PD) of avilamycin in ileal content were analyzed based on the high-performance liquid chromatography method and WinNonlin software to set up the target of PK/PD index (AUC0–24h/MIC)ex based on sigmoid Emax modeling. The PK parameters of AUC0–24h, Cmax, and Tmax in the intestinal tract were 428.62 ± 14.23 h μg/mL, 146.30 ± 13.41 μg/ml,, and 4 h, respectively. The target of (AUC0–24h/MIC)ex for bactericidal activity in intestinal content was 36.15 h. The PK-PD cutoff value (COPD) was defined as 8 μg/ml and calculated by Monte Carlo simulation. The dose regimen designed from the PK-PD study was 5.2 mg/kg mixed feeding and administrated for the treatment of C. perfringens infection. Five respective strains with different MICs were selected as the infection pathogens, and the clinical cutoff value was defined as 0.125 μg/ml based on the relationship between MIC and the possibility of cure (POC) following nonlinear regression analysis, CART, and “Window” approach. The CBP was set to be 0.25 μg/ml and selected by the integrated decision tree recommended by the Clinical Laboratory of Standard Institute. The formulation of the optimal regimens and CBP is good for clinical treatment and to control drug resistance.

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Section: Methodsmentioning

confidence: 99%

Optimal Regimens and Clinical Breakpoint of Avilamycin Against Clostridium perfringens in Swine Based on PK-PD Study

Huang¹,

Luo²,

Xu³

et al. 2022

Front. Pharmacol.

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show abstract

“…dose of 40 mg kg )1 bw (avilamycin A was dissolved in 0.5 ml glycerol formal/chicken) and blood samples collected at different time periods after dosing. Plasma samples were analyzed for avilamycin A using an HPLC method (Sunderland, 2004) with modifications. One milliliter of plasma and 5 ml of dichloromethane-acetone (1:1 v ⁄ v) were mixed and then centrifuged at 1500 g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Poster Session 2

2012

Vet Pharm & Therapeutics

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Determination of total avilamycin residues as dichloroisoeverninic acid in porcine muscle, fat, and liver by LC-MS/MS

et al. 2018

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Determination of avilamycin A and B in pig faeces by solid phase extraction and reverse-phase HPLC assay

Cited by 5 publications

References 9 publications

Optimal Regimens and Clinical Breakpoint of Avilamycin Against Clostridium perfringens in Swine Based on PK-PD Study

Optimal Regimens and Clinical Breakpoint of Avilamycin Against Clostridium perfringens in Swine Based on PK-PD Study

Poster Session 2

Determination of total avilamycin residues as dichloroisoeverninic acid in porcine muscle, fat, and liver by LC-MS/MS

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