Determination of Aspirin and Its Major Metabolites in Human Urine by Gas Chromatography–Mass Spectrometry, II

Mongillo, J.A.; Paul, J.

doi:10.1006/mchj.1996.1300

Cited by 9 publications

(3 citation statements)

References 8 publications

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“…However, these methods often suffer from disadvantages, including the lack of specificity inherent in nonchromatographic analytical methods for drugs in biological samples (Steyn, 1979), low sensitivity or long running time (Pedersen, 1979;Huang et al, 2003;Liu and Smith, 1996;Yamamoto et al, 2006;Siebert and Bochner, 1987;Kunkel and Watzig, 1999) and complex and time-consuming analytical procedures and/or derivatization (Steyn, 1979;Mongillo and Paul, 1997;Pirker et al, 2004). Some methods (Wolfram and Bjornsson, 1980) which can satisfy the quantitation of one drug in biological fluids selectively and sensitively cannot be applied to simultaneous determination of the two drugs.…”

Section: Introductionmentioning

confidence: 98%

Simultaneous determination of dipyridamole and salicylic acid in human plasma by high performance liquid chromatography‐mass spectrometry

Wang¹,

Xu²,

Zhang³

et al. 2007

Biomedical Chromatography

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A sensitive, rapid and simple high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method for simultaneous determination of dipyridamole and salicylic acid in human plasma has been developed and validated. After the addition of diazepam and rosiglitazone as internal standard (IS), plasma samples were prepared by liquid-liquid extraction followed by an isocratic elution with methanol:2 mM ammonium acetate buffer (pH 4.25; 70/30, v/v) on a Shimadzu VP-ODS C(18) column (5 microm, 150 x 2.0 mm I.D.). Detection was performed on a quadrupole mass spectrometer with ESI interface operating in the positive-ion mode for dipyridamole and negative-ion mode for salicylic acid. Calibration curves were linear (r(2) > 0.99) over the concentration range 10-2500 ng/mL for dipyridamole and 30-4000 ng/mL for salicylic acid with acceptable accuracy and precision, respectively. The intra- and inter-batch precisions were less than 15% of the relative standard deviation. The limits of detection of dipyridamole and salicylic acid were 1 and 15 ng/mL, respectively. The validated HPLC-ESI-MS method was successfully applied to a preliminary pharmacokinetic study of fixed-dose combination of sustained-release dipyridamole/aspirin in Chinese healthy male volunteers.

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Section: Introductionmentioning

confidence: 98%

Simultaneous determination of dipyridamole and salicylic acid in human plasma by high performance liquid chromatography‐mass spectrometry

Wang¹,

Xu²,

Zhang³

et al. 2007

Biomedical Chromatography

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“…Both the metabolites of aspirin exhibit a broad spectrum of biological activity, hence, it is considered desirable to determine them in biological samples. The determination of aspirin has extensively been carried out and also recently been reported [13], however, very few attempts have been made to determine metabolites 2,3-and 2,5-DHB of aspirin and techniques such as High performance liquid chromatography (HPLC) coupled with UV or electrochemical determination, Gas chromatography-mass spectrometry and spectrofluorimetry are used [14][15][16][17][18]. However, these techniques require heavy and expensive instruments with sophisticated processes, tedious time consuming pretreatment, derivatization and utilize organic solvents for separation.…”

Section: Introductionmentioning

confidence: 99%

Graphene modified glassy carbon sensor for the determination of aspirin metabolites in human biological samples

Purushotham

Gupta

Goyal

2015

Talanta

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“…The sample techniques employed previously for salicylate metabolites have involved sample concentration by evaporation of extracts, which would also remove the volatile parent compounds. 9 Therefore, the study hypothesis was that gross skin contamination as part of a decontamination study would yield urine levels of MeS and BeS sufficient to evaluate systemic availability with less opportunity for dietary confounders. In this study, the development of a novel method for the detection of MeS and BeS in urine is described, as well as its implementation in a proof-of-principal study in which MeS and BeS was quantified in the urine of six volunteers following application to skin.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Chemical Simulants in Urine: A Useful Tool for Assessing Emergency Decontamination Efficacy in Human Volunteer Studies

James

Collins

Amlôt

et al. 2020

Prehosp. Disaster med.

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Introduction: To date, all human studies of mass-casualty decontamination for chemical incidents have relied on the collection and analysis of external samples, including skin and hair, to determine decontamination efficacy. The removal of a simulant contaminant from the surface of the body with the assumption that this translates to reduced systemic exposure and reduced risk of secondary contamination has been the main outcome measure of these studies. Some studies have investigated systemic exposure through urinary levels of simulant metabolites. The data obtained in these studies were confounded by high background concentrations from dietary sources. The unmetabolized simulants have never been analyzed in urine for the purposes of decontamination efficacy assessment. Study Objective: Urinary simulant analysis could obviate the need to collect skin or hair samples during decontamination trials and provide a better estimate of both decontamination efficacy and systemic exposure. The study objective therefore was to determine whether gross skin contamination as part of a decontamination study would yield urine levels of simulants sufficient to evaluate systemic availability free from dietary confounders. Methods: In this study, a gas chromatography-tandem mass spectrometry method was developed for the analysis of two chemical simulants, methyl salicylate (MeS) and benzyl salicylate (BeS), in urine. An extraction and sample clean-up method was validated, enabling quantitation of these simulants in urine. The method was then applied to urine collected over a 24-hour period following simulant application to the skin of volunteers. Results: Both MeS and BeS were present in all urine samples and were significantly increased in all post-application samples. The MeS levels peaked one hour after skin application. The remaining urinary levels were variable, possibly due to additional MeS exposures such as inhalation. In contrast, the urinary excretion pattern for BeS was more typical for urinary excretion curves, increasing clearly above baseline from four hours post-dose and peaking between 12.5 and 21 hours, a pattern consistent with dermal absorption and rapid excretion. Conclusion: The authors propose BeS is a useful simulant for use in decontamination studies and that its measurement in urine can be used to model systemic exposures following skin application and therefore likely health consequences.

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Determination of Aspirin and Its Major Metabolites in Human Urine by Gas Chromatography–Mass Spectrometry, II

Cited by 9 publications

References 8 publications

Simultaneous determination of dipyridamole and salicylic acid in human plasma by high performance liquid chromatography‐mass spectrometry

Simultaneous determination of dipyridamole and salicylic acid in human plasma by high performance liquid chromatography‐mass spectrometry

Graphene modified glassy carbon sensor for the determination of aspirin metabolites in human biological samples

Analysis of Chemical Simulants in Urine: A Useful Tool for Assessing Emergency Decontamination Efficacy in Human Volunteer Studies

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