Determination of aspirin and its major metabolites in plasma by high-performance liquid chromatography without solvent extraction

124

“…A h.p.l.c. method was used to measure diclofenac, salicylate [13] and paracetamol [14]. The coefficients of variation for all solutes in the assay procedures were less than 5%.…”

Section: Methodsmentioning

confidence: 99%

Disappearance kinetics of solutes from synovial fluid after intra‐ articular injection.

Owen

Francis

Roberts

1994

124

“…Plasma aspirin concentrations were measured directly by reversed phase high performance liquid chromatography after protein precipitation with 30% perchloric acid (Rumble & Roberts, 1981). Acetylsalicylate was separated from salicylate and other metabolites on a C18 column using 50% methanol/50% (0.072%) orthophosphoric acid as the mobile phase (flow rate 2.5 ml/min).…”

Section: Introduction Methodsmentioning

confidence: 99%

Aspirin metabolism and efficacy in postoperative dental pain.

Seymour¹,

Williams²,

Ward³

et al. 1984

1 Aspirin 1200 mg was compared with placebo in a randomised, double-blind, crossover study in 15 patients with postoperative pain after removal of impacted lower third molars. 2 Over a 5 h investigation period, patients reported significantly less pain (P < 0.01) after treatment with aspirin, than after treatment with placebo. 3 Peak concentrations of aspirin occurred at 15 min after dosage. 4 Significant negative correlations were observed between plasma aspirin esterase activity and both AUC aspirin (r = -0.904, P < 0.001) and AUC analgesia (r = -0.91, P < 0.001). Similarly, a significant correlation was observed between AUC aspirin and AUC analgesia (r = 0.96, P < 0.001). 5 Evidence from this study would suggest that an individual's pain relief in postoperative dental pain is determined by the rate of aspirin hydrolysis to salicylate.

“…In 1971, Gelber et al determined the ratio of monoacetyldapsone (MADDS) and dapsone (DDS) (Rumble et al, 1981) (Hanson et al, 1981). This was followed by alkalinisation, extraction into diethyl ether and evaporation to dryness of an aliquot of the organic phase.…”

Section: Introductionmentioning

confidence: 99%

A rapid method for determination of acetylation phenotype using dapsone.

Philip

Roberts

Rogers

1984

A rapid, simple one‐stage protein precipitation method for the estimation of plasma dapsone (DDS) and monoacetyldapsone (MADDS) concentration by high‐pressure liquid chromatography (h.p.l.c.) is described. Its performance in the assignment of acetylator phenotype was compared with a reference h.p.l.c. method utilising an extraction procedure and internal standard. The rapid h.p.l.c. technique combined with measurement of the plasma MADDS/DDS ratio is, in our opinion, the method of choice for the determination of the acetylator phenotype in population studies.