Determination of ascorbic acid and its related compounds in foods and beverages by hydrophilic interaction liquid chromatography

Tai, Akihiro; Gohda, Eiichi

doi:10.1016/j.jchromb.2007.03.024

Cited by 70 publications

(33 citation statements)

References 28 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In general, ACN is a weaker solvent that provides higher retention than methanol under HILIC conditions. ACN was often selected over methanol because methanol was considered to be too strong as an elution solvent, leading to poor retention [58]. ACN usually provided sharper peaks than methanol [52].…”

Section: Mobile Phase Composition Impact On Hilic Selectivitymentioning

confidence: 99%

Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

Hao¹,

Xiao

Weng

2008

J of Separation Science

245

152

View full text Add to dashboard Cite

The retention mechanism and chromatographic behavior for different polar analytes under hydrophilic interaction chromatography (HILIC) conditions have been studied by application of different mobile phases and stationary phases to various analytes at different temperatures. In addition to the commonly accepted mechanism of analyte liquid-liquid partitioning between mobile phase and water-enriched solvent layer which is partially immobilized onto the surface of the stationary phase, hydrogen-bonding, hydrophobic interaction, and ion-exchange interactions may also be involved. The predominant retention mechanism in HILIC separation is not always easily predictable. It can depend not only on the characteristics of the analytes but also on the selection of mobile and stationary phase compositions. The objective of this review is to evaluate the potential application of column temperature and mobile phase composition toward improving HILIC selectivity. The functional groups from analyte structures, stationary phase materials and organic mobile phase solvents will be highlighted.

show abstract

Section: Mobile Phase Composition Impact On Hilic Selectivitymentioning

confidence: 99%

Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

Hao¹,

Xiao

Weng

2008

J of Separation Science

245

152

View full text Add to dashboard Cite

show abstract

“…The proposed method showed a good sensitivity; our limit of detection was low compared to previous papers. Tai and Gohda (2007) and Burini (2007) obtained a limit of detection around 0.3 mg/l, whereas Versari et al (2004) and Rodríguez-Comesaña et al (2002) reported values slightly higher (1.2 and 1.6 mg/l, respectively).…”

Section: Resultsmentioning

confidence: 89%

“…Different types of HPLC columns, including C 18 (Burini, 2007;Chen & Sato, 1995;de Quirós, López-Hernández, & Simal-Lozano, 2001;Furusawa, 2001;Garrido-Frenich, Hernández-Torres, Belmonte-Vega, Martínez-Vidal, & Plaza-Bolaños, 2005;Nojavana et al, 2008) bonded-phase NH 2 (Rodríguez-Comesaña, García-Falcón, & Simal-Gándara, 2002), polymeric columns (Lopes, Drinkine, Saucier, & Glories, 2006) and diol columns (Tai & Gohda, 2007) have been reported in the literature for the analysis of ascorbic acid in foods. GC-based methods have also been demonstrated to be suitable for determining ascorbic acid in foodstuffs (Silva, 2005).…”

Section: Introductionmentioning

confidence: 99%

A screening method for the determination of ascorbic acid in fruit juices and soft drinks

2009

View full text Add to dashboard Cite

“…3). sample sucrose [26] HILIC method was satisfactorily applied to the determination of ascorbic acid and related compounds in foods and beverages [27]. The chromatographic analyses were carried out on a diol column using acetonitrile and water with ammonium acetate as mobile phase.…”

Section: Diol Stationary Phasesmentioning

confidence: 99%

Application of hydrophilic interaction chromatography in phytochemical analysis

Petruczynik¹,

Waksmundzka‐Hajnos²

2013

Acta Chromatographica

View full text Add to dashboard Cite

Summary. Hydrophilic interaction chromatography (HILIC) is a liquid chromatographytechnique when a polar stationary phases -silica or polar bonded phases and aqueous mobile phase containing amount of a less polar solvent (often acetonitrile) is applied. HILIC is important for the separation of highly polar substances including biologically active compounds, such as drugs, amino acids, peptides, proteins, nucleosides, neurotransmitters, etc. HILIC is also the appropriate method for analysis of plant extract polar components such as carbohydrates, amino acids, peptides, phenolic acids, flavonoids and some alkaloids. Plant extracts are usually multicomponent mixtures of compounds of wide polarity range, which often cannot be analyzed by use of a single separation and detection method because of the high chemical diversity. Good results are obtained by use of coupled method, e.g., reversed-phase liquid chromatography (RP-LC) and HILIC mode separation. The elution order in HILIC is usually opposite to that in RP separations. This orthogonality determines one of the advantages of HILIC -the possibility of creating multidimensional separations. An important feature of HILIC is the improved sensitivity with electrospray mass spectrometry. This is significant for the analysis of components existing in small concentration in multicomponent mixtures. The high acetonitrile content also gives additional advantage of HILIC -faster separations due to the lower viscosity of HILIC eluents compared to standard RP ones. The presented review deals with the optimization of HILIC separations and application of the method for analysis of plant extracts components.

show abstract

Determination of ascorbic acid and its related compounds in foods and beverages by hydrophilic interaction liquid chromatography

Cited by 70 publications

References 28 publications

Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

A screening method for the determination of ascorbic acid in fruit juices and soft drinks

Application of hydrophilic interaction chromatography in phytochemical analysis

Contact Info

Product

Resources

About