Determination of Ascochyta blight disease in chickpea using real-time PCR

Bayraktar, Harun; Özer, Göksel; Aydoğan, Abdulkadir; Palacıoğlu, Gülsüm

doi:10.1007/s41348-016-0017-0

Cited by 14 publications

(18 citation statements)

References 26 publications

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“…Similar studies on the screening of chickpea materials for resistance to Ascochyta rabiei were performed by many researchers [28,34,35]. Disease reaction of the genotypes evaluated to Ascochyta blight was also quanti ed by using a standard curves constructed by amplifying known amounts of target DNA by real-time PCR method developed in a previous study [23]. The primer pairs HEF1/2 ampli ed a PCR fragment of 82 bp in size from plant materials infected with A. rabiei.…”

Section: Resultsmentioning

confidence: 94%

“…However, these techniques have serious restrictions, especially the visual assessment of disease symptoms may show signi cant variations both between researchers and between different assessments of the same researcher [11,18,29]. In a previous study, we observed a strong relationship between disease assessment and quanti cation of pathogen infection in resistant and susceptible cultivars based on a quantitative real-time PCR assay [23]. Our results indicated that this approach may be a useful tool for the evaluation of breeding material for disease resistance during initial phases of infection as an optional approach to visual scoring and disease management.…”

Section: Resultsmentioning

confidence: 97%

“…Quanti cation of the target DNA qPCR analysis was carried out in LightCycler 96 Real-Time PCR System (Roche Diagnostics) using speci c primers (HEF1/2, CTTATGGGCTCCGGTCCA AG/GCTTCCTGTGGTTGTCAG) for Eukaryotic elongation factor 1 EF-1 alpha gene (23). PCR mixture was prepared in a total volume of 20 µL using 2× FastStart Essential Green Master Mix (Roche Diagnostics), 0.25 µM of each primer, 5 µL of template DNA (20 ng/µL).…”

Section: Inoculation and Disease Assessmentsmentioning

confidence: 99%

“…The method has been extensively used for accurate monitoring of disease progression in infected plant tissues as an optional method to visual scoring of disease severity [21,22]. Quantitative analysis of A. rabiei infection in chickpea has been developed by Bayraktar et al [23]. This study evaluated the e cacy of Real-time PCR technique for selecting resistant breeding material to Ascochyta blight.…”

Section: Introductionmentioning

confidence: 99%

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The use of Real-Time PCR Assay for Screening Chickpea Genotypes Resistant to Ascochyta Rabiei

Özer

Palacıoğlu

Aydoğan

et al. 2021

Preprint

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Ascochyta blight, caused by Ascochyta rabiei is a devastating disease of chickpea worldwide. Breeding for host resistance is an efficient means to reduce the damage by this pathogen. This study evaluated the utility of Real-time polymerase chain reaction (PCR) assay for screening chickpea genotypes for resistant to blight disease. Eight days after inoculation, the resistance level of 84 chickpea genotypes was quantified by Real-time PCR technique using a standard curve constructed by amplifying different known amounts of target DNA and compared with disease scores based on visual assessment. A significant variation was statistically found among chickpea genotypes with respect to disease reactions. The quantity of target DNA in infected samples varied from 0.004–83.37 ng. The results demonstrated a strong relationship between visual scoring of disease severity and quantification of the target DNA in chickpea genotypes. Tüb-35, Tüb-47, Tüb-26, Tüb-82, Tüb-65 and Tüb-69 genotypes were found highly resistant to Ascochyta blight based on the results of both assays utilized for screening chickpea genotypes for resistance. The real-time PCR assay could be used for quantifying disease progression in plant tissues and screening chickpea genotypes as a potential alternative to visual scoring in plant resistance breeding programs.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 97%

Section: Inoculation and Disease Assessmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The use of Real-Time PCR Assay for Screening Chickpea Genotypes Resistant to Ascochyta Rabiei

Özer

Palacıoğlu

Aydoğan

et al. 2021

Preprint

Self Cite

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“…Advanced molecular detection techniques such as the real time polymorphic chain reaction, loop mediated isothermal amplification (LAMP) method have also been developed (Notomi et al, 2000). Bayraktar et al (2016) developed a real time PCR method to detect down to 1 pg of A. rabiei gDNA, a limit of just 0.1 pg of A. rabiei gDNA was detected with a LAMP assay, with the advantage of the visualization of color change with the naked eye (Chen et al, 2016).…”

Section: Necrotrophic Fungal Pathogens Of Grain Legumesmentioning

confidence: 99%

Advanced Diagnostic Approaches for Necrotrophic Fungal Pathogens of Temperate Legumes With a Focus on Botrytis spp.

2019

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Plant pathogens reduce global crop productivity by up to 40% per annum, causing enormous economic loss and potential environmental effects from chemical management practices. Thus, early diagnosis and quantitation of the causal pathogen species for accurate and timely disease control is crucial. Botrytis Gray Mold (BGM), caused by Botrytis cinerea and B. fabae , can seriously impact production of temperate grain legumes separately or within a complex. Accordingly, several immunogenic and molecular probe-type protocols have been developed for their diagnosis, but these have varying levels of species-specificity, sensitivity and consequent usefulness within the paddock. To substantially improve speed, accuracy and sensitivity, advanced nanoparticle-based biosensor approaches have been developed. These novel methods have made enormous impact toward disease diagnosis in the medical sciences and offer potential for transformational change within the field of plant pathology and disease management, with early and accurate diagnosis at the point-of-care in the field. Here we review several recently developed diagnostic tools that build on traditional approaches and are available for pathogen diagnosis, specifically for Botrytis spp. diagnostic applications. We then identify the specific gaps in knowledge and current limitations to these existing tools.

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Development of a sequence-characterized amplified region marker for detection of Ascochyta rabiei causing Ascochyta blight in chickpea

2019

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Determination of Ascochyta blight disease in chickpea using real-time PCR

Cited by 14 publications

References 26 publications

The use of Real-Time PCR Assay for Screening Chickpea Genotypes Resistant to Ascochyta Rabiei

The use of Real-Time PCR Assay for Screening Chickpea Genotypes Resistant to Ascochyta Rabiei

Advanced Diagnostic Approaches for Necrotrophic Fungal Pathogens of Temperate Legumes With a Focus on Botrytis spp.

Development of a sequence-characterized amplified region marker for detection of Ascochyta rabiei causing Ascochyta blight in chickpea

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