Development of a sequence-characterized amplified region marker for detection of Ascochyta rabiei causing Ascochyta blight in chickpea

Baite, Mathew S.; Upadhyay, Balendu K.; Dubey, S. C.

doi:10.1007/s12223-019-00711-5

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…In the BLAST analysis of the DNA sequence of the fragment amplified from the SCAR marker, there was no significant percentage of sequences matched to the other sequences from onions and other species; therefore, additional analysis is required. SCAR markers have been used to distinguish between various cultivars and species [28,29]. SCAR markers can be used to select individuals with specific traits and to identify disease-resistant individuals.…”

Section: Discussionmentioning

confidence: 99%

Development of Molecular Markers Associated with Resistance to Gray Mold Disease in Onion (Allium cepa L.) through RAPD-PCR and Transcriptome Analysis

Kim

Park

Park³

et al. 2021

Horticulturae

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Onions (Allium cepa L.) are one of the most consumed vegetable crops worldwide and are damaged by several fungal diseases in the field or during storage. Gray mold disease caused by the necrotrophic pathogens Botrytis cinerea and Botrytis squamosa is a disease that reduces the productivity and storage life in onions. However, it is difficult to control gray mold disease in onions by using physical and chemical methods. Breeding resistant onions against gray mold disease can reduce the damage caused by pathogens, reduce the labor required for control, and reduce environmental pollution caused by fungicides. However, onions have a large genome size (16Gb), making them difficult to analyze, and have a biennial cycle, resulting in a very long breeding period. Therefore, in this study, markers were developed to shorten the onion breeding period. First, random amplified polymorphic DNA (RAPD) was performed to confirm the genetic relationship between the gray mold disease-resistant and -susceptible lines through a dendrogram. In addition, the sequence characterized amplified region (SCAR)-OPAN1 marker to select resistant lines was developed using a polymorphic RAPD fragment. Second, the RNA-seq of the gray mold-resistant and -susceptible onion lines were analyzed using NGS technology. Using the RNA-seq results and DEG and GO analyses were performed, and the variants, such as SNPs and indels, were analyzed to develop a selectable marker for the resistant line. This study developed the SNP-3 HRM marker for selecting gray mold disease-resistant lines by using the SNPs present in the aldo-keto reductase (AKR) gene with high expression levels in these lines. The SCAR-OPAN1 and SNP-3 HRM markers developed in this study could be used to select gray mold disease-resistant onions in breeding programs to reduce the damage caused by gray mold disease.

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Section: Discussionmentioning

confidence: 99%

Development of Molecular Markers Associated with Resistance to Gray Mold Disease in Onion (Allium cepa L.) through RAPD-PCR and Transcriptome Analysis

Kim

Park

Park³

et al. 2021

Horticulturae

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“…are substantially variable; hence, PCR amplification and sequencing of this region can be used to confidently diagnose blight diseases in legumes (Barve et al, 2003). A sequence-characterized amplified region marker developed by Baite et al (2020) was specific enough to differentiate A. rabiei from eight other chickpea fungal pathogens. This method is sensitive enough to amplify 196-bp amplicons using conventional PCR and real-time PCR from 0.5 ng and 1.0 pg genomic DNA, respectively.…”

Section: Diag Nos Ismentioning

confidence: 99%

Ascochyta rabiei: A threat to global chickpea production

Singh

Kumar

Purayannur

et al. 2022

Molecular Plant Pathology

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The necrotrophic fungus Ascochyta rabiei causes Ascochyta blight (AB) disease in chickpea. A. rabiei infects all aerial parts of the plant, which results in severe yield loss. At present, AB disease occurs in most chickpea‐growing countries. Globally increased incidences of A. rabiei infection and the emergence of new aggressive isolates directed the interest of researchers toward understanding the evolution of pathogenic determinants in this fungus. In this review, we summarize the molecular and genetic studies of the pathogen along with approaches that are helping in combating the disease. Possible areas of future research are also suggested. Taxonomy kingdom Mycota, phylum Ascomycota, class Dothideomycetes, subclass Coelomycetes, order Pleosporales, family Didymellaceae, genus Ascochyta , species rabiei. Primary host A. rabiei survives primarily on Cicer species. Disease symptoms A. rabiei infects aboveground parts of the plant including leaves, petioles, stems, pods, and seeds. The disease symptoms first appear as watersoaked lesions on the leaves and stems, which turn brown or dark brown. Early symptoms include small circular necrotic lesions visible on the leaves and oval brown lesions on the stem. At later stages of infection, the lesions may girdle the stem and the region above the girdle falls off. The disease severity increases at the reproductive stage and rounded lesions with concentric rings, due to asexual structures called pycnidia, appear on leaves, stems, and pods. The infected pod becomes blighted and often results in shrivelled and infected seeds. Disease management strategies Crop failures may be avoided by judicious practices of integrated disease management based on the use of resistant or tolerant cultivars and growing chickpea in areas where conditions are least favourable for AB disease development. Use of healthy seeds free of A. rabiei , seed treatments with fungicides, and proper destruction of diseased stubbles can also reduce the fungal inoculum load. Crop rotation with nonhost crops is critical for controlling the disease. Planting moderately resistant cultivars and prudent application of fungicides is also a way to combat AB disease. However, the scarcity of AB‐resistant accessions and the continuous evolution of the pathogen challenges the disease management process. Useful websites https://www.ndsu.edu/pubweb/pulse‐info/resourcespdf/Ascochyta%20blight%20of%20chickpea.pdf https://saskpulse.com/files/newsletters/180531_ascochyta_in_chickpeas‐compressed.pdf http://www.pulseaus.com.au/growing‐pulses/bmp/chickpea/ascochyta‐blight http://agriculture.vic.gov.au/agriculture/pests‐diseases...

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“…The most important biotic stress factor affecting chickpea yield worldwide is blight disease caused by Ascochyta rabiei (Baite et al, 2020). The pathotypes of Ascochyta rabiei which infects all above-ground plant organs, including leaves, petioles, young branches and capsules, show a high pathogenic variation (Kabakçı & Özer, 2021; Salotti & Rossi, 2021).…”

Section: Introductionmentioning

confidence: 99%

Promjene osjetljivosti starih i novoregistriranih kultivara slanutka (Cicer arietinum L.) na snijet prouzročenu patotipovima Ascochyta rabiei (Pass.) Labr.

Keçeli

2023

Poljoprivreda (Online)

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Blight disease, caused by Ascochyta rabiei (Pass.) Labrousse (teleomorph=Didymella rabiei), is one of the most important biotic stress factors affecting chickpea produc tion worldwide. The high variation in disease severity among different chickpea cul tivars and the decrease in the resistance of the cultivars over time make it necessary to test the cultivars regularly. The aim in this research was to determine and evalu ate the changes in the susceptibility of chickpea cultivars, which were developed in different years and widely cultivated, against A. rabiei pathotypes in Turkey. A three replication pot experiment was conducted in a randomized plot design in the climate chamber in 2021. Fifteen registered chickpea cultivars (including one susceptible and one susceptible control cultivar) and four pathotypes of chickpea blight disease agent A. rabiei were used in the study. While Pathotype-IV was determined as the most aggressive, it was followed by the Pathotype-III, Pathotype-II, and Pathotype-I, respectively. The Azkan cultivar, included as a Tolerant (T) control in the experiment, had the Mid-Susceptible/Susceptible (MS/S) values, which can be explained by the decrease in resistance over time. However, it is opined that the main reason for the better resistance values of Akçin-91, registered in 1991, and Gökçe, registered in 1997, was provoked by the genetic basis of these cultivars, when compared to the recently registered cultivars.

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Development of a sequence-characterized amplified region marker for detection of Ascochyta rabiei causing Ascochyta blight in chickpea

Cited by 8 publications

References 13 publications

Development of Molecular Markers Associated with Resistance to Gray Mold Disease in Onion (Allium cepa L.) through RAPD-PCR and Transcriptome Analysis

Development of Molecular Markers Associated with Resistance to Gray Mold Disease in Onion (Allium cepa L.) through RAPD-PCR and Transcriptome Analysis

Ascochyta rabiei: A threat to global chickpea production

Promjene osjetljivosti starih i novoregistriranih kultivara slanutka (Cicer arietinum L.) na snijet prouzročenu patotipovima Ascochyta rabiei (Pass.) Labr.

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