Determination of As(III), As(V), monomethylarsonic acid, dimethylarsinic acid and arsenobetaine by HPLC–ICP–MS: analysis of reference materials, fish tissues and urine

Wróbel, Katarzyna; Parker, Barry; Ss, Kannamkumarath; Ja, Caruso

doi:10.1016/s0039-9140(02)00404-6

Cited by 86 publications

(41 citation statements)

References 33 publications

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“…The mechanisms of As incorporated into fish are unclear; there are only a few studies about the detoxification mechanism (Wrobel et al 2002, Bears et al 2006). Regarding F -, studies analyzing its biotransference from water to fish tissues are also scarce (Haque et al 2012, Cao et al 2013, Puntoriero et al 2014b.…”

Section: Introductionmentioning

confidence: 99%

HISTOPATHOLOGICAL CHANGES IN LIVER AND GILLS OF Odontesthes bonariensis INHABITING A LAKE WITH HIGH CONCENTRATIONS OF ARSENIC AND FLUORIDE (CHASICÓ LAKE, BUENOS AIRES PROVINCE)

Puntoriero¹,

Cirelli²,

Volpedo³

2018

Rev. Int. Contam. Ambie.

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Water bodies in the Chaco-Pampean plain contain arsenic (As) and fluoride (F -) occurring naturally. Chasicó Lake, located in the southwest of Buenos Aires Province, has high concentrations of both elements (As, 0.05-0.41 mg/L; F -, 6.74-8.54 mg/L). Fish can be considered as indicators of environmental pollution. The aim of this study was to analyze whether the presence of high concentrations of As and F -found in the liver and gills of Odonthestes bonariensis could be associated to histological changes found in these tissues. Fish were sampled from different locations of the lake. Tissue samples (liver and gills) were subdivided and subjected to an acid digestion to determine As and to an alkaline digestion to determine F -. Morphological changes were assessed using routine histological techniques. As concentrations in the liver were higher than in the gills, while the gills showed higher F -concentrations. The histological examination of the liver and gills showed alterations that could correspond to the effects of the high As and F -concentrations found in these tissues. The toxicity of these elements is due to oxidative stress. Silverside is one commercially relevant fish in Buenos Aires and a key species in the food chain in the region. Therefore, further histopathological studies are relevant to guarantee this fish's health and safety for human consumption.Palabras clave: histopatología, elementos traza, pejerrey, Argentina RESUMEN Los cuerpos de agua de la llanura Chaco-Pampeana contienen arsénico (As) y fluoruro (F -) cuyo origen es natural. El Lago Chasicó, ubicado en el sudoeste bonaerense, presenta elevadas concentraciones de ambos elementos (As: 0.05-0.41 mg/L; F -: 6.74-8.54 mg/L). Los peces pueden considerarse como indicadores de contaminación ambiental. El objetivo de este estudio fue analizar si la presencia de altas concentraciones de As y F -halladas en hígado y branquias de Odonthestes bonariensis pudiera estar asociada con los cambios histológicos encontrados en estos tejidos. Los peces fueron colectados en diferentes localidades del lago. Se subdividieron muestras de tejidos (hígado y branquias) y se sometieron a una digestión ácida para determinar As y a una digestión Rev. Int. Contam. Ambie. 34 (1) 69-77, 2018 DOI: 10.20937/RICA.2018 M.L. Puntoriero et al. 70alcalina para determinar F -. Los cambios morfológicos se determinaron mediante técnicas histológicas de rutina. En hígado se hallaron concentraciones de As más altas que en branquias, mientras que las branquias mostraron concentraciones de F -más elevadas. El examen histológico del hígado y las branquias mostró alteraciones que podrían corresponder a los efectos de las altas concentraciones de As y F -halladas en esos tejidos. La toxicidad de estos elementos se debe al estrés oxidativo. El pejerrey es uno de los peces comerciales más importantes de Buenos Aires y una de las especies clave de la cadena alimenticia en la región, por lo que resultan relevantes estudios histopatológicos adicionales para asegurar la salud de los ...

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Section: Introductionmentioning

confidence: 99%

HISTOPATHOLOGICAL CHANGES IN LIVER AND GILLS OF Odontesthes bonariensis INHABITING A LAKE WITH HIGH CONCENTRATIONS OF ARSENIC AND FLUORIDE (CHASICÓ LAKE, BUENOS AIRES PROVINCE)

Puntoriero¹,

Cirelli²,

Volpedo³

2018

Rev. Int. Contam. Ambie.

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“…The most widely used analytical methods for element speciation are hyphenated techniques, favoured for both the efficient separation and elemental-specific detection 6 . Most suitable method currently used for arsenic speciation are based on the combination of HPLC with very sensitive and element-specific detection methods such as high-performance liquid chromatography with a flame atomic absorption spectrophotometer (HPLC/AAS) 7 , high performance liquid chromatography with atomic fluorescence spectroscopy (HPLC/AFS) 8 , or high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC/ICP-MS) 9 . To improve sensitivity and eliminate matrix effects, hydride generation (HG) technique has been employed as a convenient link with the chromatographic separation 10 .…”

Section: Introductionmentioning

confidence: 99%

“…The developed method made use of NaBH 4 or KBH 4 to produce arsines to separate arsenic from the liquid products of the reaction. These arsines were subsequent detected by a variety of detectors to become on-line combinations such as HPLC/HG/AAS 12 , HPLC/HG/AFS 13 , or HPLC/HG/ICP-MS 9 . For samples containing only inorganic arsenic, i.e., As III and As V , chromatographic separation is not necessary.…”

Section: Introductionmentioning

confidence: 99%

Determination of inorganic arsenic species by hydride generation atomic absorption spectrophotometry and cathodic stripping voltammetry

Chooto¹,

Wararattananurak²,

Kangkamano³

et al. 2015

ScienceAsia

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Flow injection-hydride generation-atomic absorption spectrophotometry (FI-HG-AAS) and square wave cathodic stripping voltammetry (SWCSV) were compared to detect inorganic arsenic species in lemongrass and turmeric. Two species, arsenite (As III ) and arsenate (As V ), were considered as they are known to occur in most terrestrial plants. As III and total water-soluble inorganic arsenic (TAs inorg ) were determined under different conditions.As III was selectively determined by using a soft generation condition, i.e., low HCl concentration, whereas TAs inorg was determined after pre-reduction of As V to As III with a KI/ascorbic acid mixture. The As V content was estimated as the difference between both measurements. Under optimal conditions, the limits of detection (LOD) by FI-HG-AAS were 0.02 and 0.03 µg/l for As III and TAs inorg , respectively. Relative standard deviations (n = 9) of less than 4% were obtained for both inorganic arsenic species. The accuracy was also verified by analysing spiked samples and certified reference material: CTA-VTL-2 (Virginia Tobacco leaves). The recoveries of both species were found to be between 90 and 115%. The determination of inorganic arsenic species by SWCSV in the samples is based on the formation of a copper-arsenic intermetallic compound at the hanging mercury drop electrode (HDME) during the preconcentration step. Only As III was deposited on the Hg electrode when Cu was present in the HCl medium. TAs inorg can be determined by reducing As V to As III with sodium thiosulphate. As V is quantified as the difference. At optimum conditions, the LOD for As III and As V were 0.5 and 0.4 µg/l, respectively. Relative standard deviations (n = 10) of less than 5% were obtained and the method was validated by analysing the spiked samples and certified reference material. FI-HG-AAS showed better LOD than SWCSV for both inorganic arsenic species. There was, however, a strong agreement between TAs values obtained by using FI-HG-AAS and SWCSV technique in lemongrass, turmeric, and the certified reference material (CTA-VTL-2). As V was the main inorganic arsenic species found in lemongrass and turmeric. The results confirm that the As content of both samples do not exceed the food safety limits for Thailand and several countries.

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“…Methanol-water mixtures and sometimes chloroform are often used as extracting solvents for arsenic species from biological tissues [1,[4][5][6][7][8][9][10][11][12]. Extraction of organic arsenic species from biological tissues, especially from fish tissues, has been successfully performed using agitation [5,8], sonication [1,6,7,9,11,12], microwave heating [4,11], Soxhlet-extraction [11], and accelerated solvent extraction [10]. After extraction, the most commonly employed technique for the arsenic speciation is separation of the individual species by high-performance liquid chromatography (HPLC) combined with/without hydride-generation followed by detection with inductively coupled plasma-mass spectrometry (ICP-MS) [1,4,[6][7][8][9][10][11] or atomic fluorescence spectrometry (AFS) [5,12].…”

Section: Introductionmentioning

confidence: 99%

Speciation of arsenic compounds in fish and oyster tissues by capillary electrophoresis‐inductively coupled plasma‐mass spectrometry

Yeh

Jiang

2005

Electrophoresis

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A capillary electrophoresis-inductively coupled plasma-mass spectrometric (CE-ICP-MS) method for the speciation of six arsenic compounds, namely arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine is described. The separation has been achieved on a 70 cm length x 75 microm ID fused-silica capillary. The electrophoretic buffer used was 15 mM Tris (pH 9.0) containing 15 mM sodium dodecyl sulfate (SDS), while the applied voltage was set at +22 kV. The arsenic species in biological tissues were extracted into 80% v/v methanol-water mixture, put in a closed centrifuge tube and kept in a water bath, using microwaves at 80 degrees C for 3 min. The extraction efficiencies of individual arsenic species added to the sample at 0.5 microg As/g level were between 96% and 107%, except for As(III), for which it was 89% and 77% for oyster and fish samples, respectively. The detection limits of the species studied were in the range 0.3-0.5 ng As/mL. The procedure has been applied for the speciation analysis of two reference materials, namely dogfish muscle tissue (NRCC DORM-2) and oyster tissue (NIST SRM 1566a), and two real-world samples.

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Determination of As(III), As(V), monomethylarsonic acid, dimethylarsinic acid and arsenobetaine by HPLC–ICP–MS: analysis of reference materials, fish tissues and urine

Cited by 86 publications

References 33 publications

HISTOPATHOLOGICAL CHANGES IN LIVER AND GILLS OF Odontesthes bonariensis INHABITING A LAKE WITH HIGH CONCENTRATIONS OF ARSENIC AND FLUORIDE (CHASICÓ LAKE, BUENOS AIRES PROVINCE)

HISTOPATHOLOGICAL CHANGES IN LIVER AND GILLS OF Odontesthes bonariensis INHABITING A LAKE WITH HIGH CONCENTRATIONS OF ARSENIC AND FLUORIDE (CHASICÓ LAKE, BUENOS AIRES PROVINCE)

Determination of inorganic arsenic species by hydride generation atomic absorption spectrophotometry and cathodic stripping voltammetry

Speciation of arsenic compounds in fish and oyster tissues by capillary electrophoresis‐inductively coupled plasma‐mass spectrometry

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