Determination of antioxidant activity of Hibiscus sabdariffa and Croton caudatus in Saccharomyces cerevisiae model system

Abstract

From ancient times, plants and plant derived products are exploited as a prominent source of folkloric medicines with tremendous therapeutic potential for an array of health disorders. In the present study, ethanolic leaf extract of and were evaluated for free radical scavenging activity in model system. and showed tremendous DPPH free radical scavenging potential with an IC value of 184.88 and 305.39 µg/mL respectively at a concentration of 500 µg/mL. The ethanolic leaf extract of and also showed significant hydoxyl radical scavenging and total antioxidant activity. Ascorbic acid was used as positive control. The in vitro antioxidant activity was further supported by in vivo studies using radical scavenging mechanism in wild type and its isogenic deletion strains and . The mutant yeast cells substantially scavenged the stress generated by HO when supplemented with ethanolic leaf extract of and as evident from spot assays followed by fluorescence assay (DCF-DA) using fluorescence microscopic and intensity studies. and significantly neutralize the ROS level in yeast mutants with concomitant decrease in fluorescence intensity as compared to the untreated yeast cells. The results suggested the efficacy of and as potent antioxidants in yeast system and thus their futuristic applications in therapeutics.

Reversing UVB‐induced photoaging with Hibiscus sabdariffa calyx aqueous extract

“…In the study by Pattnaik et al, Saccharomyces cerevisiae cells were stimulated by H 2 O 2 and treated with ethanolic extract of H. sabdariffa. 45 With this extract, the ROS content induced by H 2 O 2 reduced by more than 50%. Hibiscus sabdariffa calyx aqueous extract could achieve the same effect at 2 mg mL −1 , however HSCAE was prepared by aqueous extraction.…”

“…In another study, H. sabdariffa was also reported to reduce ROS. In the study by Pattnaik et al ., Saccharomyces cerevisiae cells were stimulated by H 2 O 2 and treated with ethanolic extract of H. sabdariffa . With this extract, the ROS content induced by H 2 O 2 reduced by more than 50%.…”

Sulfonated (1→6)-β-d-Glucan (Lasiodiplodan)

“…As shown in Table 3, the reference standard, menadione (1,4-naphthoquinone or vitamin K3), demonstrated lethal cytotoxicity against S. cerevisiae strains, even at very low concentrations. In fact, menadione is an important agent that induces oxidative stress, which contributes to the increase of the production of reactive oxygen species (ROS), and causes ROS levels to exceed the endogenous antioxidant capacity (21,32). On the other hand, LAS-N and the sulfonated samples did not demonstrate lethal cytotoxicity against the S. cerevisiae strains at the evaluated concentrations.…”

“…The potential to induce oxidative stress in the LAS-N and LAS-S samples was evaluated based on the protocols described by Castro et al (20) and Subhaswaraj et al (21) with adaptations. Assays were conducted in 96-well ELISA plates.…”

Effect of Croton caudatus Geiseler Aqueous Root Extract on Reproductive and Biochemical Parameters in Male Wistar Rats

“…22The ethanolic leaf extract of CCG showed antioxidative properties with highly significant activity of reactive hydroxyl scavenging and total antioxidant activity decreased the level of reactive oxygen species (ROS) generated in the stress response system of Saccharomyces cerevisiae model system. (23) Apart from this, CCG also used in the treatment of arthritis, pain relief, stomach disease treatment (24), antidiabetic, and antimalarial treatment (18).…”