Determination of Antimicrobial Residues in Honey by Liquid Chromatography Tandem Mass Spectrometry

Tölgyesi, Ádám; Barta, Enikő; Sohn, Mary; Sharma, Virender K.

doi:10.1007/s12161-018-1166-5

Cited by 19 publications

(4 citation statements)

References 27 publications

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“…However, in most of the literature, aminoglycosides are determined only in two or three matrices such as muscle, eggs, milk [ 23 ]; muscles, liver, kidney [ 24 , 25 ]; honey and royal jelly [ 7 ]; honey and milk [ 26 ]; muscle, honey, milk [ 27 ]; or wastewater samples [ 28 ]. Various sample purification techniques are described in the literature, such as ion-exchange SPE sorbents [ 7 , 29 ], hydrophilic–lipophilic balance SPE sorbents [ 25 , 26 , 30 ], and non-polar SPE sorbents [ 31 ]. LC-MS/MS was described as the most common detection method [ 24 , 26 , 32 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Aminoglycoside Antibiotics: A Challenge in Food Control

2023

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Aminoglycosides are a widely used group of antibiotics in veterinary medicine. However, misuse and abuse of these drugs can lead to residues in the edible tissues of animals. Due to the toxicity of aminoglycosides and the exposure of consumers to the emergence of drug resistance, new methods are being sought to determine aminoglycosides in food. The method presented in this manuscript describes the determination of twelve aminoglycosides (streptomycin, dihydrostreptomycin, spectinomycin, neomycin, gentamicin, hygromycin, paromomycin, kanamycin, tobramycin, amikacin, apramycin, and sisomycin) in thirteen matrices (muscle, kidney, liver, fat, sausages, shrimps, fish honey, milk, eggs, whey powder, sour cream, and curd). Aminoglycosides were isolated from samples with extraction buffer (10 mM NH4OOCH3, 0.4 mM Na2EDTA, 1% NaCl, 2% TCA). For the clean-up purpose, HLB cartridges were used. Analysis was performed using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) with a Poroshell analytical column and a mobile phase of acetonitrile and heptafluorobutyric acid. The method was validated according to Commission Regulation (EU) 2021/808 requirements. Good performance characteristics were obtained for recovery, linearity, precision, specificity, and decision limits (CCα). This simple and high-sensitivity method can determine multi-aminoglycosides in various food samples for confirmatory analysis.

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Section: Introductionmentioning

confidence: 99%

Analysis of Aminoglycoside Antibiotics: A Challenge in Food Control

2023

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“…Unfortunately, suggested LC-MS methods in the literature incorporate laborintensive sample preparation steps. Highly polar molecular features of the AMGs have directed the researchers to develop LC methods by using ion-pairing reagents combined with octadecylsilane (C18) columns ( Tölgyesi et al, 2018) or by using hydrophilic chromatography (HILIC) columns ( Asakawa et al, 2018;Chen et al, 2017;Saluti et al, 2018). The use of ion-pairing additives is mostly preferred to combat the retention problem of AMGs (El Hawari et al, 2017;Megoulas & Koupparis, 2004).…”

Section: Introductionmentioning

confidence: 99%

Single pot in situ aqueous derivatization and subsequent determination of streptomycin and dihydrostreptomycin residues in honey by means of mass spectrometry

et al. 2023

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“…Since residual AAGs can be found in animal-derived foods, the European Union has established maximum residue limits (MLRs) for these drugs, as shown in Table . Several methods have been described to determine AAGs in food, mainly using chromatography, capillary electrophoresis, and immunoassay. − However, there are few methods using flow or microflow techniques. ,− Fluorimetry is frequently used as a detection method, but these compounds do not present native fluorescence. Diverse derivative reagents have been used for this purpose .…”

Section: Introductionmentioning

confidence: 99%

Usefulness of Hybrid Magnetoliposomes for Aminoglycoside Antibiotic Residues Determination in Food Using an Integrated Microfluidic System with Fluorometric Detection

Écija-Arenas

Román-Pizarro

Fernández-Romero

2021

J. Agric. Food Chem.

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A new microfluidic approach using hybrid magnetoliposomes (h-MLs) containing hydrophobic magnetic nanoparticles (Fe 3 O 4 @AuNPs-C12SH) and encapsulated N-acetylcysteine has been developed in this research to determine aminoglycoside antibiotic (AAG) residues in food using o-phthalaldehyde. Four AAGs, kanamycin, streptomycin, gentamicin, and neomycin, have been used as model analytes. The h-MLs have been used for reagent preconcentration and were retained using an external electromagnet device in the reaction/detection zone in a microfluidic system, inserted into the sample chamber of a conventional fluorimeter. The formation of a fluorescent isoindole derivate caused an increase in the luminescence signal, which was proportional to the analyte concentration. The dynamic range of the calibration graph was 0.1−1000 μmol L −1 , expressed as AAG concentration, with an 8.7 nmol L −1 limit of detection for kanamycin and a sampling frequency of 8 h −1 . The method was applied to determine AAG residues in milk and meat samples with recovery values between 87.2 and 107.4%.

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Determination of Antimicrobial Residues in Honey by Liquid Chromatography Tandem Mass Spectrometry

Cited by 19 publications

References 27 publications

Analysis of Aminoglycoside Antibiotics: A Challenge in Food Control

Analysis of Aminoglycoside Antibiotics: A Challenge in Food Control

Single pot in situ aqueous derivatization and subsequent determination of streptomycin and dihydrostreptomycin residues in honey by means of mass spectrometry

Usefulness of Hybrid Magnetoliposomes for Aminoglycoside Antibiotic Residues Determination in Food Using an Integrated Microfluidic System with Fluorometric Detection

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