Determination of antimicrobial effect, antioxidant activity and phenolic contents of desert truffle in Turkey

Abstract

Terfezia boudieri Chatin (Scop.) Pers., is a famous macrofungus in the world as well as in Turkey for its pleasant aroma and flavour. People believe that this mushroom has some medicinal properties. Therefore, it is consumed as food and for medicinal purposes. Chloroform, acetone and methanol extracts of T. boudieri were tested to reveal its antimicrobial activity against four Gram-positive and five Gram-negative bacteria, and one yeast using a micro dilution method. In this study, the highest minimum inhibitory concentration (MIC) value was observed with the acetone extract (MIC, 4.8 µg/mL) against Candida albicans. Maximum antimicrobial effect was also determined with the acetone extract (MIC, 39-78 µg/mL). The scavenging effect of T. boudieri on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals was measured as 0.031 mg/mL at 5 mg/mL concentration, and its reducing power was 0.214 mg/mL at 0.4 mg/mL. In addition, the phenolic contents were determined as follows: the catechin was 20 mg/g, the ferulic acid was 15 mg/g, the p-coumaric acid was 10 mg/g, and the cinnamic acid was 6 mg/g. The results showed that T. boudieri has antimicrobial activity on the gram negative and positive bacteria as well as yeast, and it also has a high antioxidant capacity. Therefore, T. boudieri can be recommended as an important natural food source.

Comparative assessment of phytochemical composition, antioxidant and anticholinesterase activities of two Basidiomycota Truffle Fungi from Turkey

“…The obtained results support the previous studies about antioxidant activity of truffles [18,19,22,28,35].…”

“…They are rich in protein (amino acids), minerals, fatty acids, and carbohydrates [13]. In addition to their nutritional properties, truffle species show [22]. The obtained results are consistent with the previous studies.…”

Medical Characteristics of Arid-Semi Arid Truffle (Terfezia and Picoa) in the Elazığ-Malatya region of Turkey

“…As previously discussed, indigenous populations have long used the extract of desert truffles as a remedy for eye diseases, including trachoma [16][17]25,34]. It seems that the antimicrobial activity of T. boudieri are changeable as reported by other researchers [18,22,[31][32][33]. This may be indicative of the broad spectrum antibiotic compound in the truffles and due to the use of different solvent and test microorganism.…”

“…Methanolic extract of Terfezia truffles had antimicrobial activity against gram positive bacteria including B. subtilis and S. aureus [18]. Chloroform, acetone, methanol extracts of T. boudieri were observed to be more effective against bacteria and yeast (32)(33). The extract of T. boudieri did not show any activity of B. megaterium, E. coli, S. aureus, C. albicans, C. glabrata, Epidermophyton spp.…”

Truffle and truffle-like fungi from continental Africa

“…This species also appears to have significant antimicrobial properties. In 2013, Doðan and Aydin [60] tested extracts of T. boudieri against nine different bacteria and one yeast. Extracts showed antimicrobial properties against all tested bacteria, although the most pronounced reported impact was on the yeast Candida albicans.…”