Determination of Antigenic Proteins of Housedust Mites in 90 Dogs Suffering from Atopic Dermatitis.

Abstract

ABSTRACT. Housedust mites, Dermatophagoides pteronyssinus (D. pteronyssinus) and Dermatophagoides farinae (D. farinae), are the important causative agents of allergic diseases in human and animals. By using 165 dogs suffering from atopic dermatitis (AD), serum levels of immunogloblin E (IgE) antibody against 25 kinds of allergen including housedust mites were determined. Housedust mites were the most frequent allergen against which 90 of the 165 allergic dogs (54.5%) by IMMUNODOT assay. With the further analysis of immunoblotting assay in the 90 dogs sensitized with housedust mites, antigenic proteins of housedust mites recognized by IgE antibodies were with the apparent molecular masses of 15, 76, 90, 98, and 170-kD. Among them, the 15-kD protein that might be ident ical to Group 2 antigens (Der f2, Der p2) was prominently observed (52/90). This study indicates that about a half of dogs with AD were sensitized to housedust mites, suggesting that Group 2 antigens of housedust mites may be a major allergen in canine AD. KEY WORDS: allergen, atopic dermatitis, canine, housedust mite, IgE.

The novel high molecular weight Dermatophagoides farinae protein Zen‐1 is a major allergen in North American and European mite allergic dogs with atopic dermatitis

“…These results mirror those of previous testing in European and American dogs, studies that used either purified group 1 and 2 allergens, or whole mite extracts . In contrast to these early observations, there are higher Der f 1 and Der f 2 IgE seropositive reactions in mite allergic dogs in Japan . That Der f 2 is an important and relevant mite allergen in Japanese atopic dogs is also supported by the rapid and robust response of Df/Der f 2‐reactive dogs to immunotherapy performed with high doses of the single and pullulan‐conjugated allergen Der f 2 .…”

“…Indeed, of 16 Japanese atopic dogs with IgE against Df, six (38%) had positive IgE to Der f 1 and seven (44%) binding Der f 2 . Other Japanese authors subsequently reported that 44 and 74% of 90 HDM‐reactive dogs had IgE antibodies that recognized Der f 1 and Der f 2, respectively . The latter observations suggested that Der f 2 was a major Df allergen for dogs in Japan.…”

A pilot study of the effect of pullulan‐conjugated Der f 2 allergen‐specific immunotherapy on canine atopic dermatitis

“…7 Der f 2 and Der p 2 are major allergens of the D. farinae and D. pteronyssinus house dust mites that have been shown to be major allergens in dogs, although additional allergens may be implicated. 9 Allermmun â HDM (Nippon Zenyaku Kogyo Co. Ltd. (ZENOAQ), Tokyo, Japan), is an immunotherapeutic agent composed of recombinant Der f 2 protein conjugated with pullulan (Der f 2-P) and is commercially available in Japan. Pullulan is a large polysaccharide polymer secreted by Aureobasidium pulllans and used in various pharmaceutical formulations.…”

Proof of concept of the preventive efficacy of high‐dose recombinant mono‐allergen immunotherapy in atopic dogs sensitized to the Dermatophagoides farinae allergen Der f 2

“…That 75% of Japanese atopic dogs sensitized to the Dermatophagoides farinae house dust mite (HDM) have IgE against Der f 2, provides a rationale to develop and test adjuvanted AIT against this single allergen.…”