Determination of antibiotic susceptibility and decarboxylase activity of coagulase-negativeStaphylococcusandMacrococcus caseolyticusstrains isolated from fermented Turkish sausage (sucuk)

Abstract

The aim of this research was to detect antibiotic susceptibility patterns and amino acid decarboxylase activity of 61 presumptive coagulase‐negative Staphylococcus (CNS) strains from the Turkish dry fermented sausage (sucuk). The presumptive CNS strains were identified at species levels as 21 S. saprophyticus, 16 S. epidermidis, 10 Macrococcus caseolyticus, 4 S. xylosus, 3 S. sciuri, 2 S. hominis, 2 S. warneri, 1 S. cohnii, 1 S. pasteuri, and 1 S. vitulinus by 16S rDNA sequence analysis. S. saprophyticus and S. epidermidis species were found highly resistant to antibiotics than other species. In addition, most of the CNS and M. caseolyticus strains showed multiple antibiotic resistance profiles. None of the CNS and M. caseolyticus strains did not decarboxylate histidine, lysine, or ornithine, but only three CNS strains produced tyramine from tyrosine. A 100% correlation was found between the presence of tdc gene and tyramine production in tyraminogenic strains. Practical applications Coagulase‐negative Staphylococcus (CNS) strains play an important role in production of traditional fermented sausages such as sucuk. In this study, S. saprophyticus and S. epidermidis were found the most common isolated CNS species from sucuk. Antibiotic resistance was detected widespread in CNS and M. caseolyticus strains. Most of the CNS strains were found resistant to clinically relevant antibiotics. Antibiotic resistance strains may be dangerous for consumer health. These strains can be a potential reservoir to spread of antibiotic resistance gene between staphylococci and other species of bacteria. In addition, biogenic amine production was not found widespread in CNS strains.

Toxin Production and Resistance of Staphylococcus Species Isolated from Fermented Artisanal Dairy Products in Benin

“…But Organji et al [ 48 ] observed in their study on milk in Egypt that S. saprophyticus was the most dominant coagulase-negative strain. This difference in proportion can be explained by the fact that our samples were not fermented enough because S. saprophyticus plays a role in the fermentation process [ 49 ]. In this respect, probably no health risks are associated with all Staphylococci detected in fermented dairy products samples.…”

Genome-Based Characterization of a Plasmid-Associated Micrococcin P1 Biosynthetic Gene Cluster and Virulence Factors in Mammaliicoccus sciuri IMDO-S72

“…Also Mammaliicoccus sciuri itself, has been the subject of many taxonomical reclassifications in the past (3,6). Its current genus name refers to the ecological niche from which these bacteria are typically isolated, being a wide variety of farm and wild mammals, as well as products derived thereof (7)(8)(9)(10)(11). Although mostly exhibiting a commensal lifestyle on its animal hosts, M. sciuri has been occasionally associated as an opportunistic pathogen associated with mastitis, dermatitis, and exudative epidermitis (12)(13)(14)(15).…”