Determination of Amitrole in Environmental Water Samples with Precolumn Derivatization by High-Performance Liquid Chromatography

Abstract

Amitrole is a nonselective polar herbicide that can easily pollute ground and surface waters because of its high solubility in water. A precolumn derivatization high-performance liquid chromatographic method for amitrole analysis has been developed. Derivatization of amitrole was performed with 4-chloro-3,5-dinitrobenzotrifluoride (CNBF). The derivatization conditions and the influence of elution composition on the separation were investigated. In pH 9.5 H(3)BO(3)-Na(2)B(4)O(7) media, the reaction of amitrole with CNBF was complete at 60 degrees C after 30 min. The stability of the derivative under light irradiation and room temperature in methanol-water samples was demonstrated. The derivatized amitrole was separated on a K C(18) column (250 mm x 4.6 mm, 5 microm) at room temperature, and UV detection was applied at 360 nm. The separation of derivatized amitrole was achieved within 18 min by gradient elution mode. The method correlation coefficient was 0.9995, in concentrations ranging from 1.59 to 318 mg L(-1). The detection limit of amitrole was 0.16 mg L(-1) with a signal-to-noise ratio of 3. The proposed method was applied to the quantitative determination of amitrole in environmental water with recoveries of 92.0-103.0% and RSDs of 2.22-6.26, depending on the sample investigated. This method has good accuracy and repeatability that can be used to quantify amitrole in environmental water.

Determination of 20 Free Amino Acids in Asparagus Tin by High-Performance Liquid Chromatographic Method after Pre-Column Derivatization

“…It can react with amines in low concentration to form stable derivatives under alkaline conditions, and the excess reagent is hydrolyzed to the corresponding phenol without any other by-products and interferences Sun et al 2009;Tang et al 2009). The hydrolysis compound can be written as (CNBF)OH.…”

High-performance liquid chromatographic method for determination of amino acids by precolumn derivatization with 4-chloro-3,5-dinitrobenzotrifluoride

“…The CNBF derivatization was rapid at moderate temperature, along with the resultant derivatives being very stable and of high ultraviolet and fluorescent absorption [41][42][43][44][45][46][47]. There were not any multiple derivatives and by-products except CNBF hydrolysis compound in the reaction, while excess reagent and its hydrolysis compound could not disturb the separation.…”

Determination of tobramycin in soil by HPLC with ultrasonic-assisted extraction and solid-phase extraction

“…4-Chloro-3,5-dinitrobenzotrifluoride (CNBF) is a novel derivatization reagent, which has been known to react with primary or secondary amines in the presence of base to produce stable N-substituted-2,6-dinitro-4-(trifluoromethyl) benzamine derivatives which display satisfactory ultraviolet absorption. There were no any other multiple derivatives and by-products except CNBF hydrolysis compound in the reaction, while excess reagent and its hydrolysis compound could not disturb the separation [25][26][27][28][29].…”