Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography

Einarsson, Stefán; Josefsson, Björn; Lagerkvist, Soeren.

doi:10.1016/s0021-9673(00)91638-8

Cited by 550 publications

(224 citation statements)

References 16 publications

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“…The yields were not affected within the reaction time interval of 2-10 min. The results indicated that the derivatization rate of CEOC with amino acids was similar to that of FMOC or AEOC previously reported [16,19]. In addition, we believe that the monosubstituted form is significantly affected by the amount of CEOC added.…”

Section: Optimization For Derivatizationsupporting

confidence: 87%

“…Data reported previously indicated that the reagent itself undergoes about 50% decomposition in methanol-water solution exposed to daylight within 25 min [15] and a large number of fluorescent components that eluted later than the derviative itself have been found. 2-(9-Fluorenyl)-ethyl chloroformate (FMOC) [16,17], 1-(9-fluorenyl)-ethyl chloroformate [18], and 2-(9-anthryl)-ethyl chloroformate (AEOC) [19] reagents have also been developed for the derivatization of amino acids and peptides for chiral or non-chiral separation in LC or CE. More recently, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) has also been developed as a popular precolumn derivatization agent [20] for the determination of amino acids with satisfactory results.…”

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confidence: 99%

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Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry

You

Shan

Liang

et al. 2003

Analytical Biochemistry

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Section: Optimization For Derivatizationsupporting

confidence: 87%

mentioning

confidence: 99%

Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry

You

Shan

Liang

et al. 2003

Analytical Biochemistry

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“…The concentrations of total homocysteine and cysteine in the plasma and liver were measured by HPLC according to the method of Durand et al 14) The concentrations of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in the liver were measured by HPLC essentially according to the method of Cook et al 15) with slight modifications as described previously. 16) The activity of cystathionine -synthase (CBS) in the liver was measured according to the method of Mudd et al, 17) but using HPLC for the assay of the reaction product, cystathionine, according to the method of Einarsson et al 18) Protein was measured according to the method of Lowry et al, 19) using bovine serum albumin as the standard.…”

Section: Methodsmentioning

confidence: 99%

Suppression of Methionine-Induced Hyperhomocysteinemia by Glycine and Serine in Rats

Fukada

Shimada

Morita

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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The hyperhomocysteinemia induced by a dietary addition of 1% methionine was significantly suppressed by the concurrent addition of 1% glycine or 1.4% serine to the same degree. The methionine-induced increase in the hepatic concentration of methionine metabolites was significantly suppressed by glycine and serine, but the hepatic cystathionine -synthase activity was not enhanced by these amino acids. When the methioninesupplemented diet was changed to the methionine plus glycine or serine diet, the plasma homocysteine concentration rapidly decreased during and after the first day. The hyperhomocysteinemia induced by an intraperitoneal injection with methionine was also suppressed by concurrent injection with glycine or serine, although the effect of serine was significantly greater than that of glycine. These results indicate that glycine and serine were effective for suppressing methionine-induced hyperhomocysteinemia: serine and its precursor glycine are considered to have elicited their effects mainly by stimulating cystathionine synthesis by supplying serine, another substrate for cystathionine synthesis.

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“…FMOC contains both chromophores and fluorophores, and can be determined using emission or absorption techniques [18].…”

Section: Introductionmentioning

confidence: 99%

Use of genetic algorithms with multivariate regression for determination of gelatine in historic papers based on FT-IR and NIR spectral data

Cséfalvayová

Pelikán

Cigić

et al. 2010

Talanta

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Quantitative non-destructive analysis of individual constituents of historic rag paper is crucial for its effective preservation. In this work, we examine the potentials of mid-and near-infrared spectroscopy, however, in order to fully utilise the selectivity inherent to spectroscopic multivariate measurements, genetic algorithms were used to select spectral data derived from information-rich FT-IR or UV-VIS-NIR measurements to build multivariate calibration models based on partial least squares regression, relating spectra to gelatine content in paper. A selective but laborious chromatographic method for the quantification of hydroxyproline (HYP) has been developed to provide the reference data on gelatine content. We used 9-fluorenylmethyl chloroformate (FMOC) to derivatise HYP, which was subsequently determined using reverse-phase liquid chromatographic separation and fluorimetric detection. In this process, the sample is consumed, which is why the method can only be used as a reference method.The sampling flexibility afforded by small-size field-portable spectroscopic instrumentation combined

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Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography

Cited by 550 publications

References 16 publications

Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry

Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry

Suppression of Methionine-Induced Hyperhomocysteinemia by Glycine and Serine in Rats

Use of genetic algorithms with multivariate regression for determination of gelatine in historic papers based on FT-IR and NIR spectral data

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