Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry

You, Jing; Shan, Yichu; Liang, Zhen; Zhang, Lin; Zhang, Yukui

doi:10.1016/s0003-2697(02)00398-6

Cited by 63 publications

(15 citation statements)

References 22 publications

(22 reference statements)

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“…The effect of malting and brewing on a diversity of cereal seeds antimicrobial peptides 34 needs to be investigated more comprehensively. Beer amino acids and small peptides have been analyzed successfully using various methods 155 . However, the knowledge of peptides of approximately 5 kDa is incomplete.…”

Section: Identificationmentioning

confidence: 99%

A Review: The Role of Barley Seed Pathogenesis-Related Proteins (PRs) in Beer Production

Gorjanović

2010

Journal of the Institute of Brewing

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Pathogenesis-related proteins (PRs) are involved in the protection of dormant and germinating cereal seeds against pathogenic microorganisms and pests. Moreover, barley grain PRs have considerable technological importance in the brewing of beer. The compact structure of PRs allows them to survive the hostile conditions of the extracellular compartments, where they are usually localized, and enables the endurance of certain classes of PRs during the harsh conditions of the technological steps of beer production. Beer proteome maps reveal a dominant presence of PRs and facilitate association between particular proteins and specific beer quality traits. Current knowledge on the influence of PRs on the various aspects of beer quality has been summarized in this review.

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Section: Identificationmentioning

confidence: 99%

A Review: The Role of Barley Seed Pathogenesis-Related Proteins (PRs) in Beer Production

Gorjanović

2010

Journal of the Institute of Brewing

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“…BDD electrode was rinsed by ultrasonic before measurement and the surface area of BDD is 0.32 cm 2 . The refreshment of BDD electrode could be easily fulfilled just by electrolysis in 1 M H 2 SO 4 aqueous solution at a constant potential of 2.8 V for 10 s. Glassy carbon electrode (3 mm in diameter, CHI) was abraded with metallographic sand paper, then polished with alumina powder (1.0, 0.3, 0.05 mm) on a flat sheet glass.…”

Section: Preparation Of Electrodesmentioning

confidence: 99%

Simultaneous and Direct Determination of Tryptophan and Tyrosine at Boron‐Doped Diamond Electrode

2006

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A facile method for the simultaneous measurement of tryptophan (Trp) and tyrosine (Tyr) was firstly exploited at unmodified boron-doped diamond (BDD) electrode. The experimental results indicated that by using differential pulse voltammetry, the oxidative peaks of these two kinds of amino acids could be completely separated at BDD electrode. The peak separation of Trp and Tyr was developed to be 0.64 V when Na 2 PO 4 /NaOH buffer solution with the optimized pH 11.2 was employed. The detection limit of Trp was obtained to be 1 Â 10 À5 M, while that of Tyr was achieved to be 1 Â 10 À6 M. The present method was also evidenced to be available to the determination of real samples of amino acids. Analysis of amino acids has been attracted more and more attentions because amino acids, as the rudimental units of enzymes and proteins, are so closely related to the life. Up to now, a number of analytical methods, such as high performance liquid chromatography [1 -3], electrophoresis [4 -6], gas chromatography [7 -8], ion exchange chromatography [9 -10], spectroscopy [11 -13] and etc. were developed. However, there somewhat are limits in these methods, e.g., low sensitivity and poor selectivity, high cost, easy to deactivation by fouling and etc. So, it is a challenging research work to establish an analytical system for the direct and swift determination of amino acids with low-cost [14 -15].Several research works on the electroanalytical determination of some amino acids, including Trp and Tyr have been described in the last few years. The mercury electrodes [16] and carbon paste electrodes [17] have mainly been employed in these researches. More recently, carbon paste electrodes with electrochemical pretreatment [18] or special chemical modification [19 -20] have been developed in electroanalysis of amino acids with good performance. Unfortunately, it is still some difficult to realize the simultaneous determination of above two kinds of amino acids by direct voltammetry at those mentioned electrodes because oxidative peaks of amino acids could not be completely separated.BDD is one of the most popular electrode materials [21]. It exhibits several excellent electrochemical properties, including a wide electrochemical potential window in either aqueous or non-aqueous media, very low capacitance, and high electrochemical stability which ensures BDD with a good reproducibility. Superior to glassy carbon electrodes, it is easy to refresh the BDD electrode surfaces and it shows extremely excellent reproducibility. In addition, BDD electrodes have recently been found to show high resistance to fouling and insensitivity to dissolved oxygen. These properties make BDD a promising material for electroanalytical applications. BDD electrodes with electrochemical pretreatment or modification have been employed for the simultaneous measurement of uric acid (AA), dopamine (DA) and other biomolecules with good selectivity and sensitivity [22 -25].In the present work, we firstly realized the simultaneous and direct voltammetric...

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“…It has been implicated as a possible cause of schizophrenia in people who cannot metabolize it properly. So, many technologies have been applied to the detection of trp, such as high-performance liquid chromatography [49][50][51][52], capillary electrophoresis [53][54][55][56][57], fluorometric methods [58], chemiluminescence [59][60][61][62][63], and ultraviolet detection [64]. Although these methods have the benefits of sensitivity and accuracy, their high cost, time-consuming, and complicated operations limit their extensive application for routine trp analysis.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical determination of tryptophan at room-temperature ionic liquid-titanium carbide nanoparticle gel modified electrode

Zhang

Pan

et al. 2014

Ionics

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The novel gel composite, based on the ground o f i o n i c l i q u i d ( 1 -b u t y l -3 -m e t h y l i m i d a z o l i u m hexafluorophosphate, BMIMPF 6 ) and titanium carbide (TiC) nanoparticles, was used to modify glassy carbon electrode and applied to the electrochemical determination of tryptophan (trp). The physical and electrochemical characterizations of the BMIMPF 6 -TiC gel modified electrode were studied in detail by scanning electron microscopy, energy dispersive X-ray spectroscopy, cyclic voltammetry, and differential pulse voltammetry methods. Due to the large specific surface area and strong adsorption property of TiC nanoparticles, as well as the good ionic conductivity and strong electrocatalytic property of BMIMPF 6 , the oxidation peak current of trp at BMIMPF 6 -TiC gel modified electrode showed good linear relation with its concentrations ranging from 0.50 to 30 μmol/L and 30 to 500 μmol/L, respectively. The detection limit was 0.053 μmol/L. More importantly, the BMIMPF 6 -TiC gel modified electrode had good antiinterference ability and can be successfully applied in the electrochemical determination of trp in real food samples.

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Determination of peptides and amino acids from wool and beer with sensitive fluorescent reagent 2-(9-carbazole)-ethyl chloroformate by reverse phase high-performance liquid chromotography and liquid chromotography mass spectrometry

Cited by 63 publications

References 22 publications

A Review: The Role of Barley Seed Pathogenesis-Related Proteins (PRs) in Beer Production

A Review: The Role of Barley Seed Pathogenesis-Related Proteins (PRs) in Beer Production

Simultaneous and Direct Determination of Tryptophan and Tyrosine at Boron‐Doped Diamond Electrode

Electrochemical determination of tryptophan at room-temperature ionic liquid-titanium carbide nanoparticle gel modified electrode

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