Determination of aldehydes and ketones using derivatization with 2,4-dinitrophenylhydrazine and liquid chromatography–atmospheric pressure photoionization-mass spectrometry

Leeuwen, S.M. van; Hendriksen, L; Kärst, Uwe

doi:10.1016/j.chroma.2004.08.149

Cited by 99 publications

(56 citation statements)

References 36 publications

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“…1,4-Dinitrophenyl hydrazine (DNPH) has been frequently used to derivatize carbonyl groups, such as ketones and aldehydes, in HPLC-MS studies ( 48,49 ). Standard DNPH adducts of DHCEO were prepared by incubating the corresponding d 0 -and d 7 -DHCEO with DNPH in methanol under acidic conditions at room temperature over 12 h (see Materials and Methods).…”

Section: Characterization Of Dhceo In Cells and Tissuesmentioning

confidence: 99%

An oxysterol biomarker for 7-dehydrocholesterol oxidation in cell/mouse models for Smith-Lemli-Opitz syndrome

Korade

Rosado

et al. 2011

Journal of Lipid Research

186

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Section: Characterization Of Dhceo In Cells and Tissuesmentioning

confidence: 99%

An oxysterol biomarker for 7-dehydrocholesterol oxidation in cell/mouse models for Smith-Lemli-Opitz syndrome

Korade

Rosado

et al. 2011

Journal of Lipid Research

186

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“…Recent work has focused on the direct comparison of different sources (APPI, APCI, and ESI) with specific target compounds, e.g., polyaromatic hydrocarbons [6,7], hydrophobic peptides [8], pesticides [9,10], as well as fatty acids and lipids [4,5]. In many cases atmospheric pressure photoionization (APPI) [1,7] has demonstrated extended linear dynamic range [11], enhanced sensitivity and thus lower detection limits [6,9,[12][13][14][15], and reduced or no off-line sample cleanups [6,9] in comparison with direct APCI or ESI. Adding a dopant (dopant-assisted, DA) to the mobile phase in many cases can further increase sensitivity [2].…”

mentioning

confidence: 99%

APPI-MS: Effects of mobile phases and VUV lamps on the detection of PAH compounds

Short¹,

Cai²,

Syage³

2007

J. Am. Soc. Mass Spectrom.

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The technique of atmospheric pressure photoionization (APPI) has several advantages over electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), including efficient ionization of nonpolar or low charge affinity compounds, reduced susceptibility to ion suppression, high sensitivity, and large linear dynamic range. These benefits are greatest at low flow rates (i.e., Յ100 L/min), while at a higher flow, photon absorption and ion-molecule reactions become significant. Under certain circumstances, APPI signal and S/N have been observed to excel at higher flow, which may be due to a nonphotoionzation mechanism. To better understand APPI at higher flow rates, we have selected three lamps (Xe, Kr, and Ar) and four mobile phases typical for reverse-phase, high-pressure liquid chromatography: acetonitrile, methanol, (1:1) acetonitrile:water and (1:1) methanol:water. As test compounds, three polyaromatic hydrocarbons are studied: benzo[a]pyrene, indeno [1,2,3-c, d]pyrene and benz [a]anthracene. We find that solvent photoabsorption cross-section is not the only parameter in explaining relative signal intensity, but that solvent photo-ion chemistry can also play a significant role. Three conclusions from this investigation are: (1) [4,5]. Recent work has focused on the direct comparison of different sources (APPI, APCI, and ESI) with specific target compounds, e.g., polyaromatic hydrocarbons [6,7], hydrophobic peptides [8], pesticides [9,10], as well as fatty acids and lipids [4,5]. In many cases atmospheric pressure photoionization (APPI) [1,7] has demonstrated extended linear dynamic range [11], enhanced sensitivity and thus lower detection limits [6,9,[12][13][14][15], and reduced or no off-line sample cleanups [6,9] in comparison with direct APCI or ESI. Adding a dopant (dopant-assisted, DA) to the mobile phase in many cases can further increase sensitivity [2].A related analytical technique is atmospheric pressure laser ionization (APLI), which has shown excellent sensitivity for certain non-or low-polar compounds [3]. The enhanced sensitivity is a direct result of the high photon flux associated with a laser system, often several orders of magnitude higher than the noncoherent light sources, e.g., lamps used with APPI. The APLI source relies on one-color, two-photon (1 ϩ 1) resonantlyenhanced multiphoton ionization (REMPI), typically using a KrF excimer laser emitting light at 5.0 eV, efficiently ionizing nonpolar molecules. However, APLIis not yet widely used due to the large size, expense, and maintenance associated with the laser system. Furthermore, changing the wavelength of an excimer laser requires the replacement of the gas mixture and often re-tuning of the laser cavity, whereas with an APPI system, the wavelength can be changed by simply switching the lamp.To improve the capabilities of APPI for LC-MS, we have focused on the ion chemistry involved in and following the photoionization process. Although direct, single-photon ionization (SPI) of a compound [M] however, there is litt...

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“…These derivatives are then usually determined by high-performance liquid chromatography (HPLC) [17,18] or capillary electrophoresis (CE) [19 -22]. The use of these separative approaches, which are unsuitable for on-line and in situ monitoring, is mainly intended to remove interferences from matrix components, rather than to determine independently single aldehydes with quite similar toxic properties.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Sniffer Based on Electrodes Supported on Ion‐Exchangers for Monitoring the State of Turning Rancid of Lipids

et al. 2010

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A porous gold electrode supported on one face of an ion-exchange membrane, acting as a solid polymer electrolyte (SPE), is proposed as an amperometric sniffer for monitoring the aldehyde content in the headspace in equilibrium with lipid samples, in order to gain indication of their state of turning rancid It was used as a detector for a flow injection system in which aldehydes were injected, after preconcentration by solid phase microextraction on a SPME fiber from the gas phase standing above thermostated samples and subsequent desorption by heating. Repeatable (AE 4.5%) sharp peaks were detected over a wide linear concentration range (0.5 -200 ppm). A detection limit of about 0.03 ppm was inferred for a signal-to-noise ratio of 3, referred to hexylaldehyde (used as prototype) dissolved in squalene or vegetable oils samples. This approach was applied to the determination of volatile aldehydes from some partially oxidized cereal oils and the results found were compared with both the corresponding peroxide values and their aldehyde content determined by a conventional spectrophotometric method.

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Determination of aldehydes and ketones using derivatization with 2,4-dinitrophenylhydrazine and liquid chromatography–atmospheric pressure photoionization-mass spectrometry

Cited by 99 publications

References 36 publications

An oxysterol biomarker for 7-dehydrocholesterol oxidation in cell/mouse models for Smith-Lemli-Opitz syndrome

An oxysterol biomarker for 7-dehydrocholesterol oxidation in cell/mouse models for Smith-Lemli-Opitz syndrome

APPI-MS: Effects of mobile phases and VUV lamps on the detection of PAH compounds

Amperometric Sniffer Based on Electrodes Supported on Ion‐Exchangers for Monitoring the State of Turning Rancid of Lipids

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