Determination of Aflatoxin M1 Levels in Produced Pasteurized Milk in Ahvaz City by Using HPLC

Behfar, Abdolazim; Khorasgani, Zahra Nazari; Alemzadeh, Ziyaaddin; Goudarzi, Mehdi; Ebrahimi, R.; Tarhani, Najmedin

doi:10.17795/jjnpp-4707

Cited by 17 publications

(6 citation statements)

References 21 publications

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“…Competitive traditional biosensing techniques that are already commercially used are, for example, enzyme-linked immunosorbent assay (ELISA) [22], electrochemical sensor [23], bilayer lipid membranes (BLM) [24], high performance liquid chromatography (HPLC) [25,26], micro-electrode immunoassay [27], field immunoassay [28] and surface plasmon resonance spectroscopy (SPR) [29,30]. Compared to those traditional benchtop electrochemical instruments, the main advantages of SOI-based ring resonators are their small size, scalable mass-production and fast readout (real-time) as well as the possibility for low cost, portable devices for point-of-care applications since they rely on CMOS-compatible processes.…”

Section: Introductionmentioning

confidence: 99%

Optical Biosensors Based on Silicon-On-Insulator Ring Resonators: A Review

et al. 2019

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Recent developments in optical biosensors based on integrated photonic devices are reviewed with a special emphasis on silicon-on-insulator ring resonators. The review is mainly devoted to the following aspects: (1) Principles of sensing mechanism, (2) sensor design, (3) biofunctionalization procedures for specific molecule detection and (4) system integration and measurement set-ups. The inherent challenges of implementing photonics-based biosensors to meet specific requirements of applications in medicine, food analysis, and environmental monitoring are discussed.

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Section: Introductionmentioning

confidence: 99%

Optical Biosensors Based on Silicon-On-Insulator Ring Resonators: A Review

et al. 2019

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“…Aflatoxins are toxic compounds, mutagenimmunosuppressive and carcinogen 1 . People in developing countries are at risk ofchronic exposure to aflatoxins through contaminated foods 2 . Among aflatoxins, B1, B2, G1 and G2 are the major toxins.…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin M1 Contamination in Traditional Yoghurts Produced in Guilan Province, Iran

Nikbakht¹,

Lachiniyan²,

Rahbar³

et al. 2016

AJPRHC

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Aflatoxin M1 is a major carcinogenic compound that may be existed in dairy products. The aim of this study was to determine the occurrence of AFM1 in traditional yoghurt samples in Guilan Province (Northern Iran). Ninety samples of traditional yoghurts were collected during summer and autumn 2014. Enzyme linked Immunosorbentassay (ELISA) which is a rapid and sensitive method was used to determine the presence and levels of AFM1. 100% of the yoghurt samples were contaminated with 5 and 83 ng/kg of AFM1. In general, AFM1 in 20 samples (22.22%) were higher than the maximum tolerance limit (50 ng/kg) accepted by the European Union. It was therefore concluded that, high occurrence of AFM1 in yoghurt is a serious risk for public health.

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“…The presence of AFM1 and CAP in milk has been a concern in some countries, which promotes the emergence of determination of these chemicals using various methods, such as ELISA, HPLC, and immunochromatographic assay. For example, in a previous study [ 12 ], Behfar et al determined 100 samples of pasteurized milk from a local factory, which revealed that the concentration of AFM1 was ranged from 0.45 to 9.760 ng/L, which was below the accepted level (50 ng/L) in milk in Iran. Meanwhile, in a study in which AFM1 levels in samples were analyzed with a commercial competitive ELISA kit and HPLC, the quantification limit was 10 ng/L for ELISA combined with HPLC [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay

Tian²,

et al. 2017

BioMed Research International

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Harsh demanding has been exposed on the concentration of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. In this study, we developed a new method based on background fluorescence quenching immunochromatographic assay (bFQICA) to detect AFM1 and CAP in milk. The detection limit for AFM1 was 0.0009 ng/mL, while that for the CAP was 0.0008 ng/mL. The assay variability was determined with 3 AFM1 standards (i.e., 0.25 ng/mL, 0.5 ng/mL, and 1.0 ng/mL), and the actual detection value was 0.2497, 0.5329, and 1.0941, respectively. For the assay variability of 3 CAP standards (i.e., 0.10 ng/mL, 0.30 ng/mL, and 0.50 ng/mL), the actual detection value was 0.0996, 0.3096, and 0.4905, respectively. The recovery rate of AFM1 was 99.7%–101.7%, while that for CAP was 95.3%–97.6%. For the test stability, AFM1 and CAP showed satisfactory test stability even at month 5. Compared with the sensitivity of liquid chromatography-mass spectrometry (LC-MS) method, no statistical difference was noticed in results of the bFQICA. Our method is convenient for the detection of AFM1 and CAP in milk with a test duration of about 8 minutes. Additionally, an internal WiFi facility is provided in the system allowing for quick connection and storage in the intelligent cell phone.

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Determination of Aflatoxin M1 Levels in Produced Pasteurized Milk in Ahvaz City by Using HPLC

Cited by 17 publications

References 21 publications

Optical Biosensors Based on Silicon-On-Insulator Ring Resonators: A Review

Optical Biosensors Based on Silicon-On-Insulator Ring Resonators: A Review

Aflatoxin M1 Contamination in Traditional Yoghurts Produced in Guilan Province, Iran

Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay

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