Determination of acyclovir in plasma by column liquid chromatography with fluorescence detection

Vitreous Disposition of Two Acycloguanosine Antivirals in the Albino and Pigmented Rabbit Models: A Novel Ocular Microdialysis Technique

“…All experimental protocols followed the Association of Research in Vision and Ophthalmology (ARVO) guidelines on the use of animals. Analytical A high pressure liquid Chromatographie assay was used for the analysis of ganciclovir and acyclovir (21). The system comprised of a model 510 solvent delivery module, Rheodyne injector, and a FL-45 fluorescence detector at ex.…”

Development and validation of a new reversed-phase ion pairing liquid chromatographic method with fluorescence detection for penciclovir analysis in plasma and aqueous humor

“…Furthermore, with the selected stationary phase, it is possible to perform separations and enhance method sensitivity at low pH, without deterioration of column performances. Indeed, purines are not fluorescent in the non-ionised form [29] and fluorescence is heavily pH-dependent with best results obtained under strongly acidic conditions [29]. Several mobile phases were tested at different pH ranging from 2.0 to 4.0, and the best signal was obtained at pH 2.0 (Fig.…”

Simultaneous determination of aciclovir, ganciclovir, and penciclovir in human plasma by high-performance liquid chromatography with fluorescence detection

“…rescence detection [23,24,29,33,36,37,39,40,45,46,49], and tandem mass spectrometry [47,53]. However, the LLOQs for UV detection were above 50 and 200 ng/ml for GCV, and PCV, respectively, which may not be sensitive enough for pharmacokinetic studies.…”

“…Hence, this complicates analysis and requires using a highly selective analytical method. Some analytical methods have been proposed for individual measurement of ACV [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37], GCV [38][39][40][41][42][43][44][45], and PCV [46][47][48]. Other techniques have been developed for simultaneous determination of ACV and GCV [49][50][51], valacyclovir and ACV [52], or valganciclovir, and GCV [53], through high-performance liquid chromatography (HPLC) in biological fluids.…”